A kind of composite bacterial agent and its application in degrading pyrethroid pesticide residues in soil
The technology of a compound bacterial agent and fenvalerate, which is applied in the field of microorganisms, achieves the effects of broad application prospects, improved efficiency and reduced dosage
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Embodiment 1
[0033] A kind of composite bacterial agent, it is prepared according to the following steps:
[0034] Take fenvalerate 100mg, glucose 50g, crop straw powder 40g, soybean meal 20g, K 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g, MnSO 4 0.2g, FeSO 4 Add 0.1g to water, adjust the pH to 6.8, and dilute to 1L to prepare the acclimatization medium;
[0035] Sphingomonas seed solution (1 × 10 8 individuals / ml) according to the inoculation amount of 5% (volume ratio), transferred to the acclimatization medium, and cultivated at 30°C for 6h; then the Bacillus licheniformis seed liquid (1×10 8 individual / ml), Rhodopseudomonas palustris seed solution (1×10 8 individual / ml) and Micrococcus luteus seed solution (1×10 8 individual / ml) were mixed according to the volume ratio of 3:2:2, and then transferred to the acclimatization medium according to the inoculation amount of 6% (volume ratio), cultivated at 30°C for 6h, and then added 0.02wt% fenvalerate; finally Enterobacter cloacae se...
Embodiment 2
[0040] A kind of composite bacterial agent, it is prepared according to the following steps:
[0041] Take fenvalerate 100mg, glucose 50g, crop straw powder 40g, soybean meal 20g, K 2 HPO 4 2g, KH 2 PO 4 2g, MgSO 4 0.5g, MnSO 4 0.2g, FeSO 4 Add 0.1g to water, adjust the pH to 6.8, and adjust the volume to 1L to prepare the acclimatization medium;
[0042] Sphingomonas seed solution (1 × 10 8 individuals / ml) according to the inoculation amount of 5% (volume ratio), transferred to the acclimatization medium, and cultivated at 30°C for 9h; then the Bacillus licheniformis seed solution (1×10 8 individual / ml), Rhodopseudomonas palustris seed solution (1×10 8 individual / ml) and Micrococcus luteus seed solution (1×10 8 individual / ml) were mixed according to the volume ratio of 5:3:3, and then transferred to the acclimatization medium according to the inoculation amount of 6% (volume ratio), cultivated at 30°C for 9h, and then added 0.02wt% fenvalerate; finally Enterobact...
Embodiment 3
[0047] Degradation test of pyrethroid pesticide residues in soil:
[0048] Sample selection: Sample 1: the contents of bifenthrin, fenpropathrin and cypermethrin are all 100mg / kg; sample 2: the contents of bifenthrin, fenpropathrin and cypermethrin are all 200mg / kg; sample 3: The contents of bifenthrin, fenpropathrin and cypermethrin were all 400mg / kg.
[0049] Operation process: Taking Example 1 as an example, add 100 g of the compound microbial agent into 1000 kg of soil sample, stir evenly, and ensure that the water content is about 20-30%. The contents of bifenthrin, fenpropathrin and cypermethrin were detected at 12h, 24h, 48h, 72h and 96h respectively. figure 1 It can be seen that the degradation effects of the compound bacterial agents on bifenthrin, fenpropathrin and cypermethrin are not much different, the degradation rate can reach more than 35% in 24 hours, and the degradation is basically completed in 96 hours; Figure 2-3 It can be seen that the degradation rate...
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