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Rapid and unmarked imaging method for distinguishing normal hemoglobin and hemosiderin

A technology of hemoglobin and hemoglobin, applied in the direction of material excitation analysis, Raman scattering, etc., can solve the problems of difficult clinical technology, incomplete non-specific staining, toxicity of exogenous fluorescent agents and interference labels, etc., and achieve rapid detection , to avoid the effect of marking interference

Active Publication Date: 2017-12-15
FUDAN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the imaging technology based on exogenous fluorescent labeling is very popular in the field of biomedical research, it is difficult to apply this technology to the field of biomedical research due to the toxicity and interference of exogenous fluorescent agents, incomplete labeling and non-specific staining. clinical

Method used

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  • Rapid and unmarked imaging method for distinguishing normal hemoglobin and hemosiderin
  • Rapid and unmarked imaging method for distinguishing normal hemoglobin and hemosiderin
  • Rapid and unmarked imaging method for distinguishing normal hemoglobin and hemosiderin

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Experimental program
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Embodiment Construction

[0025] The steps to build and validate the imaging method are as follows:

[0026] (1) Build a femtosecond pump-probe spectral imaging system, such as figure 1 As shown, the wavelength of the modulated pump light is fixed at 1040 nm, and the wavelength of the probe light is adjustable from 680 to 1030 nm. The time delay between the pump and probe pulses can be scanned by adding an optical delay line in the pump light path. to control. Take fresh blood from the fingers of volunteers and dilute it with PBS as a standard sample of normal red blood cells. The pump-probe spectral imaging system was used to collect the time decay curves of two standard samples at different detection wavelengths, and the results are shown in figure 2 (b). It was found that the time-decay curves of normal hemoglobin and hemosiderin were significantly different, in which hemosiderin showed a positive signal at all detection wavelengths (the pump light would cause the absorption of the detection lig...

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Abstract

Belonging to the technical field of nonlinear optical imaging, the invention specifically relates to a rapid and unmarked imaging method for distinguishing normal hemoglobin and hemosiderin. Based on the finding that hemoglobin and hemosiderin have distinct pump-probe spectrum characteristics, hemoglobin and hemosiderin can be discriminated directly at the same time, and further by combination with the stimulated raman scattering technology and pump-probe light technology, rapid and unmarked imaging of tissue, normal hemoglobin and hemosiderin can be carried out selectively. The method provided by the invention can achieve real-time distinguishing and monitoring of normal hemoglobin and hemosiderin, and image the relative distribution of normal hemoglobin and hemosiderin in fresh tissues.

Description

technical field [0001] The invention belongs to the technical field of nonlinear optical imaging, and in particular relates to an imaging method for distinguishing normal hemoglobin and hemosiderin. Background technique [0002] Hemosiderin is a ferritin complex converted from erythrocytes and hemoglobin through phagocytosis, usually present in macrophages or other cells, and sometimes also extracellularly. Hemosiderin deposition may occur after tissue hemorrhage. Therefore, there is a huge difference between hemosiderin and normal hemoglobin, and the new real-time and accurate positioning of hemosiderin and the technical method of distinguishing hemosiderin from normal hemoglobin are of great value. [0003] Nonlinear optical microscopy has been developed rapidly in the past decade and has been widely used in the fields of biomedicine and materials science. The most widely used of these are two-photon fluorescence and second-harmonic microscopy, but these two techniques c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 季敏标张莉丽邹翔陈亮毛颖
Owner FUDAN UNIV