Wild imitation cultivation method of dendrobium officinale tissue culture seedlings

A technique of imitating wild cultivation and Dendrobium candidum, which is applied in the field of imitation wild cultivation of Dendrobium candidum tissue culture seedlings, can solve the problems of limited time for planting seedlings, waste of Dendrobium seedlings, increased manpower, material and financial costs, and avoid root system breakage and damage, prevent ground diseases and insect pests, and shorten production time

Inactive Publication Date: 2017-12-26
河南联源生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, regarding the tissue culture of Dendrobium candidum, the technology has gradually matured and stabilized. Since the tissue culture of Dendrobium candidum is carried out in an artificial environment, its medicinal value is far less than that of wild Dendrobium candidum, so it is necessary to use Dendrobium candidum is planted by imitating the wild method, but there are still various technical defects and drawbacks for the imitation wild cultivation method of Dendrobium candidum
[0004] For example, the invention patent with publication number 105960973A disclosed on September 28, 2016 discloses a cultivation method of Dendrobium huoshanense under natural forests. Branches are cut off from the junction with the trunk, and a triangular incision is cut at the trunk for grafting; the invention patent published on January 4, 2017 with the publication number 106258885A discloses a method for imitating wild cultivation of Dendrobium officinale, which is to use Dendrobium candidum is fixed on the moso bamboo for more than 1 year as the carrier of the epiphytic cultivation of Dendrobium candidum. In October of the lunar calendar, the seedlings of Dendrobium candidum are fixed above the bamboo nodes of moso bamboo; the invention patent with the publication number 106376443A disclosed on February 8, 2017 A kind of imitation wild planting method of Dendrobium officinale, it selects the withered upright tree, directly transplants and fixes the Dendrobium on the upright tree, plants 10 seedlings in each layer, arranges them evenly along the ring shape of the upright tree, and carries out artificial fertilization management, just imitates Part of the growth characteristics of wild Dendrobium is actually planted in the field, and the above two cases are directly planted in the wild. Due to the influence of the natural environment, the time for planting seedlings is relatively limited and the survival rate is low.
[0005] The common shortcomings of the above-mentioned various methods are: 1) the procedures are complicated, inconvenient to operate, and inefficient, especially in the unfavorable conditions in the field to bind and climb the tree (such as tree height, steep slope, cliff, etc.), the operation implementation 2) The survival rate is low and the cost is high. Grafting or binding directly to the tree in the wild will result in high mortality and slow growth. The waste of Dendrobium seedlings is serious, and the follow-up seedling replacement workload is heavy, which seriously increases manpower, Material and financial costs; 3) The imitation of wild cultivation places is very limited, not all trees or stone walls are suitable, such as trees with oil or gel, smooth-skinned trees or stone walls, etc., are not suitable for conventional methods of transplanting, It is not easy to widely promote and apply

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  • Wild imitation cultivation method of dendrobium officinale tissue culture seedlings
  • Wild imitation cultivation method of dendrobium officinale tissue culture seedlings

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A method for imitating wild cultivation of tissue culture seedlings of Dendrobium officinale includes the following steps:

[0033] A. Selection of seedlings: select and cultivate in the culture room for 60 days, and smelt the seedlings in the greenhouse for 30 days. The plant height of the tissue culture seedlings out of the bottle is more than 5cm, with 4-6 plant nodes, more than 3 roots, and strong stems. Tissue cultured seedlings of Dendrobium officinale with dark green leaves;

[0034] B. Drying seedlings: Wash the selected Dendrobium candidum tissue culture seedlings in step A and air them under astigmatism until the roots are whitish and set aside; by airing the seedlings, the hydrophilicity of the roots of the Dendrobium candidum tissue culture seedlings can be effectively improved, but the roots Attach to the wood of planting seedlings to improve root fixation effect and survival rate;

[0035] C. Cutting board: Choose wood with thick skin, longitudinal cracks, high ...

Embodiment 2

[0042] A method for imitating wild cultivation of tissue culture seedlings of Dendrobium officinale includes the following steps:

[0043] A. Selection of seedlings: select the seedlings that are cultivated in the culture room for 65 days, and the seedlings are refined in the greenhouse for 40 days, the plant height is above 5cm, the plant has 4-6 nodes, 3 or more roots, strong stems and dark green leaves Dendrobium candidum tissue culture seedlings

[0044] B. Dry seedlings: Clean the tissue culture seedlings of Dendrobium officinale selected in step A and dry them under astigmatism until the roots are whitish for use;

[0045] C. Cutting board: Choose wood with thick skin, longitudinal cracks, high moisture, no grease and gel, such as wood with bark such as fir, cypress, pine, and poplar, and cut into 20cm wide, 30cm long, center Sheets with a thickness of 6cm, such as figure 1 As shown, the board has a board xylem 2 and a board bark 3. A fixing mechanism 6 is provided on the uppe...

Embodiment 3

[0051] A method for imitating wild cultivation of tissue culture seedlings of Dendrobium officinale includes the following steps:

[0052] A. Selection of seedlings: selection of tissue culture seedlings of Dendrobium candidum that have been cultivated in a culture room for 70 days, and cultivated in a greenhouse for 50 days;

[0053] B. Dry seedlings: Clean the tissue culture seedlings of Dendrobium officinale selected in step A and dry them under astigmatism until the roots are whitish for use;

[0054] C. Cutting board: Choose wood with thick skin, longitudinal cracks, high moisture, no grease and gel, such as fir, cypress, pine, poplar and other wood with bark, and cut into 30cm wide, 50cm long, center Sheets with a thickness of 10cm, such as figure 1 As shown, the board has a board xylem 2 and a board bark 3. A fixing mechanism 6 is provided on the upper board xylem 2 side. The fixing mechanism 6 is a hook, a hanging ring or an iron nail, and the board is prepared for use;

[005...

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Abstract

The invention discloses a wild imitation cultivation method of dendrobium officinale tissue culture seedlings. The method comprises the following steps: selecting seedlings, airing seedlings, cutting boards, planting seedlings, fixing roots and performing transplanting. After the seedlings are selected, cleaned and aired, the seedlings are directly fixedly planted and bound on the cut boards with barks; then the seedlings are suspended collectively in a domestication greenhouse for domestication; and after the roots of the dendrobium officinale tissue culture seedlings are fixed, the domesticated dendrobium officinale tissue culture seedlings and the boards with barks are transplanted to a suitable field environment for wild imitation growth. The method leaves out a step that greenhouse seedbed domestication must be performed on tissue culture seedlings in traditional methods, reduces the production processes, saves a large amount of investment of manpower, material resources and financial resources, greatly reduces the production cost, and shortens the production time. The tissue culture seedlings cultivated by the wild imitation cultivation method has a high survival rate and wide transplanting range, and the method is simple and convenient, has high operability, is good in safety and reliability, and is high in survival rate, and the post-seedling phenomenon is greatly reduced.

Description

Technical field [0001] The invention relates to the technical field of imitating wild cultivation of Dendrobium officinale, in particular to a wild imitating cultivation method of Dendrobium officinale tissue culture seedlings. Background technique [0002] Dendrobium officinale is a rare and precious Chinese medicinal material. It is known as "Dendrobium", "Plant Gold" and "Healthy Soft Gold". It is listed as "Chinese Medicine" because of its efficacy, extremely rare, special living environment and outstanding nourishing effects. The first of "Nine Great Immortals" is called "Medicinal Giant Panda" by the international medicinal plant community. Especially for nourishing yin and lungs, nourishing stomach and body fluid, strengthening brain, eyesight, and replenishing the five internal organs; it has special effects on excessive tobacco and alcohol, overwork, excessive nightlife, overuse of eyes and brain, hoarseness of vocal cords, etc.; for human lung cancer Cells, atrophic ga...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01N43/78A01N43/40A01N43/38A01P21/00
CPCA01G31/00A01N43/38A01N43/40A01N43/78
Inventor 王高平许东东陈绍军
Owner 河南联源生物科技股份有限公司
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