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A method for efficient extraction of quorum-sensing signaling molecules from aerobic granular sludge

An aerobic granular sludge, quorum sensing signal technology, applied in the preparation of test samples, etc., can solve the problems of ineffective protection of microbial cells, cell rupture, and high AHL concentration in the aqueous phase

Active Publication Date: 2019-10-01
ZHEJIANG UNIV
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Problems solved by technology

However, the current AHL extraction methods in the aerobic granular sludge system cannot effectively protect microbial cells, resulting in cell rupture, and the outflow of intracellular enzymes causes AHL to degrade. Does not accurately reflect the retention of AHL in extracellular polymers

Method used

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  • A method for efficient extraction of quorum-sensing signaling molecules from aerobic granular sludge
  • A method for efficient extraction of quorum-sensing signaling molecules from aerobic granular sludge
  • A method for efficient extraction of quorum-sensing signaling molecules from aerobic granular sludge

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Embodiment

[0036] The purpose of this example is to efficiently extract quorum sensing signal molecules from aerobic granular sludge, specifically according to the following steps:

[0037] Step 1, extracellular polymer hydrolysis

[0038] According to the composition of extracellular polymers in granular sludge, four different hydrolytic enzymes were selected as candidate enzymes, namely α-amylase, β-amylase, proteinase K, and lipase water. Use 4 kinds of enzymes to hydrolyze 10mL of the aerobic granular sludge to be tested, and then take 5mL of the hydrolyzed sludge to measure the sludge MLVSS in the system respectively, considering the influence of the three factors of enzyme concentration, temperature and pH on the enzymatic reaction , the same amount of the remaining aerobic granular sludge was taken, because the signal molecule has a variety of chemical structures, and its multi-component content index is not conducive to the analysis of the results of the orthogonal experiment, so...

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Abstract

The invention discloses a method of efficiently extracting quorum sensing signal molecules from aerobic granular sludge. The method can efficiently extract quorum sensing signal molecules from extracellular polymeric substances in aerobic granular sludge. The method comprises the following steps: (1) extracellular polymeric substance hydrolysis; (2) hydrolysis supernate collection; (3) liquid-liquid extraction; (4) vacuum concentration; and (5) nitrogen blowing and constant volume. The operation is convenient, and the reaction conditions are mild. The method can solve the problem of single molecular decomposition caused by release of intracellular organic matters during the process of extracting signal molecules from a granular sludge system and is an efficient and feasible signal molecule extraction method.

Description

technical field [0001] The invention relates to a method for efficiently extracting quorum sensing signal molecules from aerobic granular sludge, belonging to the field of wastewater biological treatment. Background technique [0002] Quorum sensing is the main information transmission method between microbial cells. Microorganisms secrete one or more signal molecules according to their own population density and environmental factors, and enter cells through diffusion and transporter functions and bind to specific proteins, thereby regulating the expression of downstream functional genes. Life activities such as biofilm formation, secretion of pathogenic factors, and bioluminescence. Signal molecules are mainly divided into three categories, namely N-acyl homoserine lactone (Acyl-HomoserineLactone, AHL) acting on Gram-negative bacteria, polypeptide substances acting on Gram-positive bacteria and simultaneous It acts on the AI-2 substance between Gram-positive bacteria and ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/40
Inventor 张智明俞卓栋朱亮徐向阳
Owner ZHEJIANG UNIV
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