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Controlled medicine release orientated porous composite electrospun fibrous membrane, and preparation method and application thereof

An electrospinning fiber and electrospinning technology, which is applied in the field of oriented porous composite electrospinning fiber membrane and its preparation, can solve the problems of increasing erythropoiesis and the like, and achieves accelerated wound healing speed, strong practical significance, and promotion of vascularization ability. Effect

Active Publication Date: 2018-01-09
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of DMOG has side effects, such as promoting massive angiogenesis leading to tumor formation and increasing erythropoiesis, etc.

Method used

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  • Controlled medicine release orientated porous composite electrospun fibrous membrane, and preparation method and application thereof
  • Controlled medicine release orientated porous composite electrospun fibrous membrane, and preparation method and application thereof
  • Controlled medicine release orientated porous composite electrospun fibrous membrane, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Preparation of oriented porous electrospun fiber membrane with controlled drug release

[0050] (1) Preparation and morphological characteristics of mesoporous silica nanoparticles (MSNs) and MSNs loaded with DMOG

[0051] The method of preparing mesoporous silica nanoparticles is as follows

[0052] (A) Put the Duran reagent bottle containing 500mL deionized water into the 80℃ constant temperature water bath, and then add 1.82g (about 5mmol) cetyltrimethylammonium bromide (CTAB) and 3.0g (about 81mmol) )Ammonium fluoride (NH 4 F) The etchant is sequentially added to the bottle. After the temperature of the entire reaction system stabilized for 1 hour, 9 mL of ethyl orthosilicate (TEOS, about 40 mmol) was slowly dropped into the above solution with a syringe, and the reaction was completed for 2 hours after the TEOS was added. The experiment was over.

[0053] (B) Place the obtained product overnight, transfer the upper layer mixture to a beaker, and centrifuge at 10...

Embodiment 2

[0076] The effect of the oriented porous electrospun fiber membrane with controlled drug release prepared in Example 1 on the adhesion, proliferation, migration, vascularization and expression of vascular-related genes of HUVECs:

[0077] 2.1 Cell adhesion, proliferation and migration

[0078] HUVECs were purchased from the East China Normal University cell bank and cultured in endothelial cell culture medium supplemented with 2.5% fetal bovine serum, 1% endothelial cell growth supplement and 1% penicillin-streptomycin. Cells at 37°C / 5% CO 2 Culture in an incubator, and the medium is changed every 2 days. Before the cell experiment, all the membranes were cut into round pieces that could completely match the size of the culture plate, and then the samples were sterilized with 75% ethanol for 30 minutes and washed with PBS three times.

[0079] The CCK-8 method was used to analyze the proliferation of HUVECs on the oriented porous composite fiber membrane. Set the density to 1×10 4 ...

Embodiment 3

[0092] In this example, the effect of an oriented porous electrospun fiber membrane with controlled drug release on the quality of wound repair in mice was studied.

[0093] 3.1 Oriented porous composite DS-PL film accelerates diabetic wound healing

[0094] To induce diabetes-like symptoms, 6-8 weeks of C57BL / 6 mice were intraperitoneally injected with streptozotocin (50 mg / kg body weight per day) for 5 consecutive days. The blood glucose level of the mouse was measured with a glucose meter 10 days after the injection, and if the blood glucose was higher than 20 mM, the mouse was regarded as a diabetic mouse. According to the glucose level, mice (n=12 / group) were randomly assigned to the control group, PL and DS-PL groups.

[0095] Diabetic mice (12-14 weeks) were anesthetized with inhaled isoflurane (5%), and the back hair of the mice was shaved. A round skin wound with a diameter of 8 mm was formed on the back of each mouse. Different electrospun fiber membranes (blank, PL and ...

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Abstract

The invention belongs to the field of a biological material and discloses a controlled medicine release orientated porous composite electrospun fibrous membrane. Poly-L-lactic acid serves as a matrixand has a single orientated structure, nanopores are distributed in the surface of fiber, and medicine loaded mesoporous silicon dioxide nanoparticles are distributed in the fiber. The fibrous membrane is prepared by a mixed electrostatic spinning technology, and the process is simple and easy to control. The multi-stage nano-structure, the controlled release medicines and Si ions generate a synergistic effect and can promote the angiogenesis of a wound area, so that the controlled medicine release orientated porous composite electrospun fibrous membrane accelerates the healing of diabetes wound and can serve as novel wound repair dressing.

Description

Technical field [0001] The invention belongs to the field of biological materials, and relates to an oriented porous composite electrospun fiber membrane with controllable drug release, and a preparation method and application thereof. Background technique [0002] Chronic ulcers are one of the most serious complications of diabetes. Once the skin of the feet of diabetic patients is injured, they will face the risk of amputation, which leads to increased medical costs and poor quality of life for patients. Normal skin wounds can heal in a timely and orderly manner. Compared with diabetic wounds, the typical characteristics of diabetic wounds are insufficient angiogenesis and slow wound healing. This is due to the long-term high level of glucose in the blood, which significantly reduces angiogenic factors The secretion of keratinocytes, and reduce the migration and proliferation of keratinocytes and re-epithelialization of wounds [1]. [0003] The tissue engineering that has emerge...

Claims

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Application Information

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IPC IPC(8): A61L15/42A61L15/44A61L15/20A61L15/18A61L15/26A61L27/60A61L27/02A61L27/18A61L27/56A61L27/54D04H1/728
Inventor 徐合柯勤飞任筱芝易正芳
Owner SHANGHAI NORMAL UNIVERSITY
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