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A method for screening cells modified by magnetic nanoparticles based on light-induced dielectrophoresis device

A light-induced dielectrophoresis, magnetic nanoparticle technology, applied in biochemical equipment and methods, electric/wave energy treatment enzymes, electric/wave energy treatment of microorganisms, etc. Damage and other problems, to achieve the effect of convenient optical path construction, easy promotion and application, and good accuracy

Active Publication Date: 2020-05-15
CHANGCHUN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the extraction of red blood cells in human blood is more complicated and requires fluorescent labeling, which may cause damage to the cells, resulting in poor accuracy of experimental results

Method used

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  • A method for screening cells modified by magnetic nanoparticles based on light-induced dielectrophoresis device
  • A method for screening cells modified by magnetic nanoparticles based on light-induced dielectrophoresis device
  • A method for screening cells modified by magnetic nanoparticles based on light-induced dielectrophoresis device

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Experimental program
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Embodiment Construction

[0025] like figure 1 Shown, the light-induced dielectrophoresis device that the present invention adopts comprises computer 11, projector 12, signal generator 13, optical microscope 14, optical microscope illumination source 15, charge-coupled device (CCD) 16, convex lens 17, mirror 18, Light-induced dielectrophoresis chip 19; further, connect the computer 11 to the projector 12, project the photoelectrode pattern through the optical system, that is, the convex lens 17, the mirror 18, and focus on the light-induced dielectrophoresis chip 19. The solution layer 24 of 19 generates a non-uniform electric field to realize cell manipulation and screening, and is connected to a charge-coupled device (CCD) 16 through an optical microscope illumination source 15, an optical microscope 14, and is projected on a computer 11 for real-time observation.

[0026] like figure 2 As shown, the light-induced dielectrophoretic chip 19 includes a glass plate 21 , an indium tin oxide conductive ...

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Abstract

The invention relates to a method for screening cells modified by magnetic nanoparticles based on a light-induced dielectrophoresis device. The light-induced dielectrophoresis device is used to screen cells modified by magnetic nanoparticles from a mixed solution, and a light-induced dielectrophoresis optical path is built. The light emitted by the light source is irradiated onto the chip through the optical path, and a non-uniform electric field is formed in the solution layer of the chip, and the cell screening is realized by utilizing the difference between yeast cells carrying magnetic nanoparticles and yeast cells. The invention can screen the same kind of cells, and the time required for the screening process is shorter, and the accuracy of screening particles is higher.

Description

technical field [0001] The invention belongs to the field of micro-nano control, and in particular relates to a method for screening cells modified by magnetic nanoparticles based on a light-induced dielectrophoresis device. Background technique [0002] In the field of manipulation of micro-nano biological particles, with the continuous development of micro-processing technology, many labs on a chip have emerged. The manipulation and separation of micro-nano biological particles has attracted extensive attention from many scientists. Many biological particles will have a certain dielectric In the case of an external electric field, these biological particles will be polarized to different degrees. If the external electric field is a non-uniform electric field, a dielectrophoretic force will be generated to make the particles move under the action of force. In recent years, dielectrophoresis technology has been introduced into the field of biological particle manipulation, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N1/16
Inventor 王作斌陈思兰杨焕洲刘梦楠董莉彤郝博王莹刘劲芸邸旭宋正勋翁占坤许红梅
Owner CHANGCHUN UNIV OF SCI & TECH