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Methods and SNP detection kits for predicting palm oil yield of a test oil palm plant

A technology of experimental oil and palm oil, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of narrow breeding materials, etc.

Active Publication Date: 2018-01-12
SIME DARBY PLANTATION INTPROP SDN BHD
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Problems solved by technology

However, applying this approach to oil palms is impractical because commercial palms tend to arise from genetically narrow breeding material

Method used

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  • Methods and SNP detection kits for predicting palm oil yield of a test oil palm plant
  • Methods and SNP detection kits for predicting palm oil yield of a test oil palm plant
  • Methods and SNP detection kits for predicting palm oil yield of a test oil palm plant

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Experimental program
Comparison scheme
Effect test

Embodiment

[0113] Sampling and DNA preparation

[0114] For resequencing, 132 palms belonging to 59 origins held at the Sime Darby Plantation Research and Development Center in Malaysia were sampled. Sampling was extended to genome-wide association study (also known as GWAS) mapping populations derived from the Deli dura x AVROS pisifera breeding population (1,045 palms) and the Nigerian dura AVROS pisifera introgression population (586 palms). Sample selection was based on good representatives of the oil-to-dry mesocarp ratio (also known as O / DM) variant and the pedigree recorded by the corresponding breeder. Total genomic DNA was isolated from unexpanded young leaves using the DNAeasy(R) Plant Mini Kit (Qiagen, Limburg, The Netherlands).

[0115] whole genome resequencing

[0116] 132 samples were pooled based on equal molar concentrations of DNA from each sample to form a sequencing DNA pool. Resequenced libraries were prepared using the HiSeq 2000(TM) Sequencing System (Illumina, ...

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Abstract

Methods for predicting palm oil yield of a test oil palm plant are disclosed. The methods comprise determining, from a sample of a test oil palm plant of a population, at least a first SNP genotype, corresponding to a first SNP marker, located in a first QTL for a high-oil- production trait and associated, after stratification and kinship correction, with the high-oil- production trait with a genome-wide -log10(p-value) of at least 4.0 in the population or having a linkage disequilibrium r2 value of at least 0.2 with respect to a first other SNP marker linked thereto and associated, after stratification and kinship correction, with the high-oil-production trait with a genome-wide -log10(p-value) of at least 4.0 in the population. The methods also comprise comparing the first SNP genotype to a corresponding first reference SNP genotype and predicting palm oil yield of the test plant based on extent of matching of the SNP genotypes.

Description

technical field [0001] The present application relates to a method for predicting palm oil yield of experimental oil palm plants, and more particularly, to a method for predicting palm oil yield of experimental oil palm plants and predicting palm oil yield of experimental oil palm plants according to such method A SNP detection kit, the method comprising determining at least a first single nucleotide polymorphism (SNP) genotype of the experimental oil palm plant from a sample of the experimental oil palm plant of a population of oil palm plants, the first SNP The genotype corresponds to a first SNP marker, the first SNP genotype of the experimental oil palm plant is compared with the corresponding first reference SNP genotype indicative of a high oil yield trait in the same genetic background as the population, and based on the experimental oil palm The degree to which the first SNP genotype of the plant matches the corresponding first reference SNP genotype is used to predict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q2600/13C12Q2600/156C12Q1/6895
Inventor C·K·特A·L·龙Q·B·克翁S·阿帕罗M·穆罕默德F·T·周D·阿普尔顿H·库拉维拉辛加姆
Owner SIME DARBY PLANTATION INTPROP SDN BHD
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