A kind of extraction method of Moringa oleifera leaf extract

An extraction method and technology of Moringa leaves, which are applied in the field of extraction of Moringa leaves extracts, can solve problems such as lack of research on Moringa leaves, restrictions on the promotion and in-depth development of Moringa protein products, and achieve improved raw material utilization and production costs The effect of low cost and simple process operation

Active Publication Date: 2021-05-14
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, due to the lack of research on the deep processing technology of Moringa leaves, the promotion and in-depth development of Moringa protein products are seriously restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A method for extracting Moringa leaf extract, comprising the following steps:

[0061] (1) Enzymatic hydrolysis with plant hydrolase: Dried fresh Moringa oleifera leaves, crushed them, passed through a 60-mesh sieve, and added deionized water to the obtained Moringa oleifera leaf powder at a ratio of 1:10 g / mL, at a rate of 6000 rpm Cut for 10 minutes, adjust the pH value to 4.4, add a compound plant hydrolase (product model Viscozyme L) whose mass is 2% of the mass of Moringa oleifera leaves, and enzymolyze at 50°C for 4 hours to obtain a suspension;

[0062] (2) Alkaline extraction combined with protease enzymatic hydrolysis: adjust the pH value of the suspension to 8.0, stir at 400 r / min at room temperature for 2 hours; then adjust the pH value of the suspension to 7.0, add 3% of the mass of Moringa oleifera leaves % protease (the amount of trypsin added accounted for 20% of the total enzyme amount, and the amount of NS37071 added accounted for 80% of the total enzym...

Embodiment 2

[0069] A method for extracting Moringa leaf extract, comprising the following steps:

[0070] (1) Enzymatic hydrolysis with plant hydrolase: Dried fresh Moringa oleifera leaves, crushed them, passed through a 60-mesh sieve, and added deionized water to the obtained Moringa oleifera leaf powder at a rate of 7000rpm according to the ratio of material to liquid 1:11 g / mL. Cut for 15 minutes, adjust the pH value to 4.7, add a compound plant hydrolase (product model Viscozyme L) whose mass is 3% of the mass of Moringa oleifera leaves, and enzymolyze at 53°C for 5 hours to obtain a suspension;

[0071] (2) Alkaline extraction combined with protease enzymatic hydrolysis: adjust the pH value of the suspension to 8.5, stir at 500 r / min at room temperature for 3 hours; then adjust the pH value of the suspension to 8.0, add 4% of the mass of Moringa oleifera leaves % protease (the amount of trypsin added accounted for 30% of the total enzyme amount, and the amount of NS37071 added accoun...

Embodiment 3

[0078] A method for extracting Moringa leaf extract, comprising the following steps:

[0079] (1) Enzymatic hydrolysis with plant hydrolase: dry fresh Moringa oleifera leaves, crush them, pass through a 60-mesh sieve, and add deionized water to the obtained Moringa oleifera leaf powder at a ratio of 1:12 g / mL, at a rate of 8000 rpm Cut for 20 minutes, adjust the pH value to 5.0, add a compound plant hydrolase (product model Viscozyme L) whose mass is 4% of the mass of Moringa oleifera leaves, and enzymolyze at 56°C for 6 hours to obtain a suspension;

[0080] (2) Alkaline extraction combined with protease enzymatic hydrolysis: adjust the pH value of the suspension to 9.0, stir at 600 r / min at room temperature for 4 hours; then adjust the pH value of the suspension to 9.0, add 5% of the mass of Moringa oleifera leaves % protease (the amount of trypsin added accounted for 40% of the total enzyme amount, and the amount of NS37071 added accounted for 60% of the total enzyme amount...

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PUM

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Abstract

The invention discloses an extraction method of Moringa oleifera leaf extract. The method adopts plant hydrolase enzymatic hydrolysis, alkaline extraction combined with protease enzymatic hydrolysis, ultrafiltration treatment, ethyl acetate extraction to remove impurities, graded alcohol precipitation and macroporous resin enrichment treatment processes for joint extraction to realize the control of Moringa oleifera leaf protein Enzymatic hydrolysis improves the utilization rate of raw materials, efficiently enriches components with strong antioxidant properties, and extracts antioxidant peptides from Moringa oleifera leaves to the greatest extent. The extraction method of the present invention has simple process operation, low production cost, and no pollution. In the obtained Moringa oleifera leaf extract, the protein content is >70%, the total sugar content is <20%, the water content is <8%, and the ORAC value is >1500 μmol Trolox equiv / g , DPPH value>600μmol Trolox equiv / g, can be used in medicine, health products and food.

Description

technical field [0001] The invention belongs to the field of deep processing of moringa leaves, and in particular relates to a method for extracting moringa leaves. Background technique [0002] The accumulation of free radicals in the body will cause various damages to the body at the molecular level, cellular level and tissue and organ level, accelerate the body's aging, fatigue and induce Alzheimer's disease, aging process, cataract, acute liver toxicity, Cardiovascular diseases, arteriosclerosis, nephritis, diabetes, rheumatism, inflammatory processes, carcinogenesis due to DNA damage, etc. [0003] Moringa is a plant of the genus Moringa in the Moringaceae family. It is native to the Himalayas in northern India and is widely planted in tropical and subtropical regions of Asia and Africa. There are 14 species of Moringa. Indian traditional Moringa ( Moringa oleifera Lam.) grows fast and distributes widely. It is the Moringa species with the largest cultivated area and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/185A61P39/06A23L33/105A61K127/00
Inventor 林恋竹赵谋明赵坤王欣朱启源
Owner SOUTH CHINA UNIV OF TECH
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