Polycitrate/chitin nano whisker tissue engineering scaffold and preparation method thereof

A technology of chitin nanocrystals and tissue engineering scaffolds, applied in additive processing, medical science, prostheses, etc., can solve the problem that polycitrate cannot be prepared by 3D printing for tissue engineering scaffolds, and achieve excellent elastic properties, compression The effect of large elastic recovery rate and uniform degradation rate

Active Publication Date: 2020-09-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problem in the prior art that polycitrate cannot be prepared by 3D printing for tissue engineering scaffolds, and to provide a polycitrate / chitin nano whisker tissue engineering scaffold that can be prepared by 3D printing technology and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation method of polycitrate / chitin nano-whiskers tissue engineering scaffold, the steps are as follows:

[0041] (1) Disperse chitin nano-whiskers in deionized water to form a dispersion liquid. Chitin nano-whiskers are needle-shaped nanoparticles obtained by hydrolyzing chitin extracted from shrimp and crab shells with hydrochloric acid. Chitin nano-crystals The average length of whiskers is 300nm, and the average diameter is 20nm;

[0042](2) Mix citric acid with 1,8-octanediol and polyethylene glycol at a molar ratio of 1:0.3:0.7, melt at 160°C, react at 135°C for 6 hours, dialyze with deionized water, and freeze-dry to remove Moisture content makes polycitrate prepolymer, joins in the dispersion liquid in the step (1) and obtains the mixture solution that solid content is 5%, wherein the mass ratio of chitin nano-whiskers and polycitrate is 0.5: 1,;

[0043] (3) The mixture solution is subjected to vacuum freeze-drying treatment in a vacuum freeze dryer,...

Embodiment 2

[0049] The preparation method of polycitrate / chitin nano-whiskers tissue engineering scaffold, the steps are as follows:

[0050] (1) Disperse chitin nano-whiskers in PBS buffer solution with a pH value of 7.4 to form a dispersion liquid. Chitin nano-whiskers are needle-like nano-whiskers obtained by hydrolyzing chitin extracted from shrimp and crab shells with hydrochloric acid. Particles, chitin nano whiskers have an average length of 300nm and an average diameter of 20nm;

[0051] (2) Mix citric acid and triblock polyether at a molar ratio of 1.5:1, melt at 160°C, react at 135°C for 12 hours, dialyze with deionized water, freeze-dry to remove water to obtain polycitrate The prepolymer is added to the dispersion in step (1) to obtain a mixture solution with a solid content of 8%, wherein the mass ratio of chitin nano whiskers to polycitrate is 0.75:1;

[0052] (3) The mixture solution is vacuum freeze-dried in a vacuum freeze dryer, wherein the drying conditions are: temper...

Embodiment 3

[0058] The preparation method of polycitrate / chitin nano-whiskers tissue engineering scaffold, the steps are as follows:

[0059] (1) Disperse chitin nano whiskers in a PBS buffer solution with a pH value of 5.0 to form a dispersion. Particles, chitin nano whiskers have an average length of 300nm and an average diameter of 20nm;

[0060] (2) Mix citric acid with 1,8-octanediol and tri-block polyether at a molar ratio of 1:0.7:0.3, melt at 160°C, react at 135°C for 8 hours, dialyze with deionized water, and freeze-dry Remove water content and make polycitrate prepolymer, join in the dispersion liquid in the step (1) and obtain the mixture solution that solid content is 3%, wherein the mass ratio of chitin nano-whiskers and polycitrate is 1.0 :1;

[0061] (3) The mixture solution is subjected to vacuum freeze-drying treatment in a vacuum freeze dryer, wherein the drying conditions are: temperature -70°C, pressure 10Pa, time 18h;

[0062] (4) adding a PBS buffer solution with ...

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Abstract

The invention relates to a poly citrate / chitin nanometer whisker tissue engineering scaffold, and a preparation method thereof. The preparation method comprises following steps: 1, chitin nanometer whisker is dispersed in a solvent so as to obtain a dispersion liquid; 2, a poly citrate performed polymer is added so as to obtain a mixed solution; 3, vacuum freeze drying is carried out; 4, a solventis added for mixing so as to obtain a poly citrate performed polymer / chitin nanometer whisker slurry; 5, 3D printing is carried out so as to obtain a poly citrate performed polymer / chitin nanometerwhisker scaffold; and 6, thermocuring crosslinking is carried out so as to obtain the poly citrate / chitin nanometer whisker tissue engineering scaffold. According to the preparation method, 3D printing is adopted to prepare a nanometer composite elastomer material with chitin nanometer whisker uniformly dispersed in a poly citrate matrix; the preparation method is simple and safe, and is friendlyto the environment; and the poly citrate / chitin nanometer whisker tissue engineering scaffold is excellent in biological degradability, biocompatibility, and mechanical properties.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds for soft tissue repair, in particular to a polycitrate / chitin nano-whisker tissue engineering scaffold and a preparation method thereof. Background technique [0002] An ideal tissue engineering scaffold should imitate the natural interstitium to the greatest extent in terms of function and structure, and provide a temporary place for cell proliferation and regeneration of new interstitial cells. Different tissues and organs of the human body have different requirements for the mechanical properties, degradation rate, pore size and porosity of tissue porous scaffolds. [0003] At present, the methods used to prepare porous tissue scaffolds mainly include particle leaching, gas foaming, phase separation / freeze drying, electrospinning, and rapid prototyping. Rapid prototyping, that is, 3D printing, mainly uses the principle of discrete stacking to design a model through 3D CAD, and then ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/18A61L27/50A61L27/56A61L27/58B33Y10/00B33Y70/00
Inventor 吉亚丽田雅玲梁凯杨庆郯志清郭永芳
Owner DONGHUA UNIV
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