A kind of lactobacillus plantarum and its application in fruit enzyme products
A technology of Lactobacillus plantarum and fruit enzymes, applied in the field of microorganisms, can solve the problem of low biological activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0067] A plant Lactobacillus ( Lactobacillus plantarum ) The collection and separation of SITCC No.10011, the steps are as follows:
[0068] (1), sample collection
[0069] Samples were taken from naturally fermented fruit and vegetable products, traditional fermented dairy products (milk fans, milk cakes, yogurt, kumiss, etc.), raw milk, dough, kefir grains (Tibetan mushroom), silage, etc., and the collected Put the sample in an ice box to refrigerate, keep it at a lower temperature and bring it back to the laboratory and place it in a refrigerator at 4°C to separate the lactic acid bacteria as soon as possible;
[0070] (2), sample pretreatment
[0071] Take 10g of solid sample (20mL of liquid sample) into a 250mL Erlenmeyer flask (containing glass beads) filled with 90mL of sterile water, shake and let stand for 20min, and set aside;
[0072] (3) Preliminary isolation of lactic acid bacteria with high DPPH free radical scavenging ability
[0073] Use sterile water to s...
Embodiment 2
[0081] The plant lactobacillus ( Lactobacillus plantarum ) SITCC No.10011 for microbiological identification
[0082] (1) Colony characteristics:
[0083] Lactobacillus plantarum ( Lactobacillus plantarum ) SITCC No.10011 strain was isolated by streaking on the MRS plate, cultured anaerobically at 37°C for 48 hours, Lactobacillus plantarum ( Lactobacillus plantarum ) SITCC No.10011 strain grows well, its colony is round, raised, with neat edges, milky white color slightly yellowish, opaque, moist and smooth surface, can be drawn when picked;
[0084] (2), Morphological characteristics of bacteria:
[0085] The Lactobacillus plantarum ( Lactobacillus plantarum ) SITCC No.10011 cells were observed by Gram staining, the results are shown in figure 1 shown, from figure 1 It can be seen from the figure that the bacteria are non-moving rods, arranged in chains of different lengths, and also in a single scattered arrangement. The size of the bacteria is generally 0.6 μm×1...
Embodiment 3
[0100] Lactobacillus plantarum ( Lactobacillus plantarum ) Growth characteristics of SITCC No.10011
[0101] (1), Lactobacillus plantarum ( Lactobacillus plantarum ) Growth curve of SITCC No.10011
[0102] The activated Lactobacillus plantarum ( Lactobacillus plantarum ) SITCC No.10011 was inserted into MRS broth liquid medium according to 2% (v / v) inoculation amount, cultured at 37°C for 16 hours, and the OD value of the culture solution was measured at 600nm every 1-2 hours, expressed as OD 600nm The value is plotted against time to obtain Lactobacillus plantarum ( Lactobacillus plantarum ) The growth curve of SITCC No.10011 in MRS broth liquid medium, the results are as follows figure 2 shown, from figure 2 It can be seen that Lactobacillus plantarum ( Lactobacillus plantarum ) SITCC No.10011 grows rapidly in MRS broth liquid medium, enters the logarithmic phase in about 3 hours, and enters the stable phase in about 10 hours;
[0103] (2), Lactobacillus planta...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com