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Preparation method and application of effective parts of Siberian white thorn polysaccharide

A technology of effective parts and Nitraria polysaccharides, applied in the preparation and application of Siberian Nitraria polysaccharides effective parts, can solve the problems of aging, carcinogenesis, liver damage, etc., and achieve the effect of strong total reducing ability

Active Publication Date: 2022-07-05
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the antioxidants currently available in the market are synthesized, which may cause aging, liver damage, carcinogenesis, etc.

Method used

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  • Preparation method and application of effective parts of Siberian white thorn polysaccharide
  • Preparation method and application of effective parts of Siberian white thorn polysaccharide
  • Preparation method and application of effective parts of Siberian white thorn polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Hot water extraction:

[0058] a. Collecting the ripe fruit of Siberian thorn, drying at a temperature of 40 °C, mechanically pulverizing, and passing through a 40-mesh sieve to obtain Siberian thorn fruit powder;

[0059] b, the powder obtained in step a is 1:8g / mL by material-to-liquid ratio, adds petroleum ether, stirs for 2 hours, extracts until the petroleum ether layer is colorless, suction filtration, and the powder is air-dried until there is no petroleum ether solvent residue, to obtain defatted Siberian thorn fruit powder;

[0060] c. Add the chloroform-methanol mixed solution with a volume ratio of 4:1 to the degreasing Siberian white thorn powder obtained in step b according to the material-to-liquid ratio of 1:5g / mL, stir at room temperature for 2 hours, extract 3 times, suction filtration, and naturally air dry , then add 90% ethanol with a material-to-liquid weight ratio of 1:10 g / mL, reflux for 3 hours, repeat until the color of the ethanol layer soluti...

Embodiment 2

[0064] Ultrasonic extraction:

[0065] a. Collecting the ripe fruit of Siberian thorn, drying at a temperature of 40 °C, mechanically pulverizing, and passing through a 40-mesh sieve to obtain Siberian thorn fruit powder;

[0066] b, the powder obtained in step a is 1:8g / mL by material-to-liquid ratio, adds petroleum ether, stirs for 2 hours, extracts until the petroleum ether layer is colorless, suction filtration, and the powder is air-dried until there is no petroleum ether solvent residue, to obtain defatted Siberian thorn fruit powder;

[0067] c. Add the chloroform-methanol mixed solution with a volume ratio of 4:1 to the degreasing Siberian thorn powder obtained in step b according to the material-to-liquid ratio of 1:5g / mL, stir at room temperature for 2 hours, extract 4 times, suction filtration, and air dry naturally , then add 90% ethanol with a material-to-liquid weight ratio of 1:10 g / mL, reflux for 3 hours, extract until the color of the ethanol layer solution i...

Embodiment 3

[0071] Cellulase Extraction:

[0072] a. Collecting the ripe fruit of Siberian thorn, drying at a temperature of 40 °C, mechanically pulverizing, and passing through a 40-mesh sieve to obtain Siberian thorn fruit powder;

[0073] b, the powder obtained in step a is 1:8g / mL by material-to-liquid ratio, adds petroleum ether, stirs for 2 hours, extracts until the petroleum ether layer is colorless, suction filtration, and the powder is air-dried until there is no petroleum ether solvent residue, to obtain defatted Siberian thorn fruit powder;

[0074] c. Add the chloroform-methanol mixed solution with a volume ratio of 4:1 to the degreasing Siberian white thorn powder obtained in step b according to the material-to-liquid ratio of 1:5g / mL, stir at room temperature for 2 hours, extract 3 times, suction filtration, and naturally air dry , then add 90% ethanol with a material-to-liquid weight ratio of 1:10 g / mL, reflux for 3 hours, extract until the color of the ethanol layer solut...

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Abstract

The invention relates to a preparation method and application of effective parts of Siberian white thorn polysaccharide. In the method, Siberian thorn fruit is dried, pulverized, degreased with petroleum ether, decolorized with chloroform-methanol, refluxed with ethanol, and then extracted by hot water extraction and ultrasonic-assisted extraction. Extraction method, cellulase extraction method, pectinase extraction method and pectinase+ultrasonic-assisted extraction method, etc., the extract is subjected to centrifugation, macroporous adsorption resin purification, concentration, absolute ethanol precipitation, centrifugation, and the polysaccharide precipitate is extracted. Vacuum freeze-drying is carried out to prepare the effective parts of Siberian white thorn polysaccharide. The effective part of the white thorn polysaccharide obtained by the method has been verified to have strong antioxidant activity and growth promotion activity of probiotics. It is of great practical significance to research and develop polysaccharide natural antioxidants and intestinal flora regulators to improve the immune function of the body. The invention can provide a basis for the application of the effective parts of the Siberian white thorn polysaccharide in the fields of food, medicine and cosmetics, the creation of healthy food with specific functional activities, and the realization of the industrial application of technical achievements.

Description

technical field [0001] The invention relates to a preparation method and application of effective parts of Siberian white polysaccharide. Background technique [0002] There are 11 species of shrubs in the genus Nitrariaceae, widely distributed in Asia, Europe, and Africa. It is distributed in Xinjiang, Qinghai, Gansu, Ningxia and Inner Mongolia in the northwest of my country. The fruits and leaves of the genus Whitethorn can be used as medicine. Siberian white thorn (Nitraria sibirica Pall.) is a plant of Tribulus terrestris, which is distributed in various regions of Xinjiang, my country. [0003] Siberian white thorn is used in traditional Chinese medicine to treat a variety of ailments, including high blood pressure, weak spleen and stomach, and menstrual disorders. At the same time, modern pharmacological studies have proved that white thorn fruit has various effects such as lowering blood pressure, lowering blood lipids, expanding coronary arteries, anti-inflammator...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P39/06A61K8/73A61Q19/08A23L33/125A23L33/21
CPCC08B37/0003A61K31/715A61P39/06A61K8/73A61Q19/08A23L33/125A23L33/21A23V2002/00A23V2200/318A23V2200/3204Y02A50/30
Inventor 阿布力米提·伊力阿衣吐逊·阿布都外力阿吉艾克拜尔·艾萨
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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