Detection method of periploca forrestii

Abstract

The invention discloses a detection method of periploca forrestii. The periploca forrestii is subjected to extraction including the steps of: 1) adding powder of periploca forrestii to a 60-80% ethanol solution according to material-to-liquid ratio of 1:20-1:30, and performing heating reflux extraction for 2-4 times, each time lasting for 50-70 min; 2) evaporative-drying an extract liquid in waterbath at 70 DEG C and adding distilled water to obtain a suspension liquid; 3) extracting the suspension liquid successively with petroleum ether, chloroform, ethyl acetate and n-butanol for 2-4 timesand collecting an n-butanol extracted part, and concentrating the n-butanol extracted part to dry to obtain the periploca forrestii extract. The detection method includes a content measuring method,which includes content detection to chlorogenic acid and periplocin. The method is used for detecting contents of the chlorogenic acid and periplocin in the periploca forrestii. The detection method is accurate and has high sensitivity, is good in repeatability and has reliable results, supplies evidence for medicinal quality control and evaluation for periploca forrestii and lays the foundation for determination of an optimum locality. The method can be used for determining an appropriate production region for the medicine during production and guiding harvesting of the medicine.

Description

technical field [0001] The present invention relates to a kind of detection method of black bone rattan, belongs to the field of medicine technology. Background technique [0002] Black bone vine belongs to the dry root or whole plant of Periploca forrestii Schltr., a plant of Asclepiadaceae. It is used by ethnic minorities in Guizhou Province. Activate collaterals, dispel wind and dampness; mainly treat rheumatoid arthritis, bruises, stomach pain, indigestion, amenorrhea, dysentery, etc. [0003] Heiguteng is one of the commonly used Miao medicines, and it is an important component of Heiguteng Zhuifeng Huoluo Capsules, Heiguteng Shenjin Tougu Liquid, and Heiguteng Shenjin Tougu Spray. At present, Heiguteng is still implementing the 2003 edition of "Guizhou Provincial Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials Quality Standards". In this standard, there are only items such as source, character, microscopic identification and physical and chemica...

AI Technical Summary

Problems solved by technology

At present, Heiguteng still implements the 2003 edition of "Guizhou Provincial Traditional Chinese Medicinal Materials and Ethnic Medicinal Materials Quality Standards". In this standard, there are only items such as source, character, microscopic identification and physical and chemical identification, and there is no qualitative and quantitative analysis of its medicinal ingredients. Testing, it is difficult to control the

Images