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Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae

A technology of microalgae Chrysanthemum carinii and unsaturated fatty acid, applied in biochemical equipment and methods, determination/inspection of microorganisms, unicellular algae, etc.

Active Publication Date: 2018-01-19
深圳市莱康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few studies involving the fatty acid composition of said algal species

Method used

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  • Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae
  • Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae
  • Methods for production of polyunsaturated fatty acids from coccolithophorid microalga pleurochrysis carterae

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Embodiment Construction

[0006] 1). The medium components used to induce the synthesis and accumulation of polyunsaturated fatty acids in the algae species

[0007] The medium used to induce the synthesis and accumulation of polyunsaturated fatty acids of the algal species comprises one of the following medium:

[0008]

[0009] Inducing said algal species to synthesize and accumulate polyunsaturated fatty acids can be achieved using the above medium in the following culture devices:

[0010] 1a. In the agar gel plate containing the above medium and 0.5%-5%;

[0011] 1b. In a 100 ml to 5 liter flask containing 50 ml to 2 liters of the above medium;

[0012] 1c. In a 2 liter to 1000 liter column photobioreactor containing the above medium;

[0013] 1d. In a flat-plate photobioreactor of 5 liters or more containing the above medium;

[0014] 2). Inducing the algal species to synthesize and accumulate polyunsaturated fatty acids

[0015] First, the coccolithophorid microalgae Pleurochrysis cartera...

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Abstract

Methods for production of polyunsaturated fatty acids (PUFAs) from coccolithophorid Pleurochrysis carterae are disclosed. This invention provides methods for producing eicosapentaenoic acid (EPA, 20:5 n-3), docosahexaenoic acid (DHA, 22: 6 n-3), stearidonic acid (SDA, 18: 4 n-3), alpha-linolenic acid (ALA, 18: 3 n-3), and oleic acid (18: 1) from Pleurochrysis carterae (Haptophyta, Prymnesiophyceae). The current invention comprises (a). culture conditions for inducing the synthesis of the PUFAs in the said organism; (b). a method to enhance the synthesis of oleic acid in the said organism. ThePUFAs produced with the current invention can be used for food, dietary supplement, cosmetic and pharmaceutical applications.

Description

Background technique [0001] Coccolithophorid microalgae are a class of unicellular plankton widely found in temperate and tropical oceans. Coccolithic marine algal blooms are a common type of marine algal blooms, with an average annual area of ​​1.4 million square kilometers between 1978 and 1986 (Brown and Yoder, 1994), and the main algal species are Emiliania huxleyi and Gephyrocapsa oceanica (Hulburt et al., 1960). Due to their widespread distribution and unique physiological characteristics, coccolithomorphic microalgae have important impacts on marine ecosystems and the global carbon cycle. As photosynthetic organisms, coccolith microalgae can not only use carbon dioxide through photosynthesis to synthesize and accumulate proteins, lipids, and other various organic compounds, they can also use carbon dioxide to synthesize calcium carbonate crystals through calcification, thus achieving long-term carbon dioxide The purpose of storage (Brownlee and Taylor, 2004; Rost and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P7/40C12Q3/00C12P7/6427C12P7/6432C12P7/6434
CPCC12N1/12C12Q3/00C12P7/6427C12P7/6432C12P7/6434
Inventor 卢凡陈佳丽邓中洋陈攀胡征宇宋立荣丰平仲
Owner 深圳市莱康生物科技有限公司
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