Pig epidemic diarrhea attenuated strain as well as culture method and application thereof

Abstract

The invention discloses a pig epidemic diarrhea attenuated strain as well as a culture method and application thereof. The preservation number of the pig epidemic diarrhea attenuated strain JS / PEDV / 2 / 2014G100 is CCTCC NO.V201728; the whole length of the strain is 27939bp; 49 nucleotides are deleted from the ORF3 gene of the strain when being compared with a PEDV virulent strain; phylogenetic analysis shows that the strain belongs to a G1 subgroup, can be proliferated without pancreatin on Vero cells, belongs to cell adaptation strains and is relatively good in cross protection, antigen property and immunogenicity; pigs infected by the strain have normal clinical symptoms, the weights of the pigs can be increased normally, no toxin removal phenomenon is caused, high-level PEDV antibodies can be generated in pig bodies, the OD450 value of a serum IgG antibody can be up to 1.2, the OD450 level of an immunized 60d antibody can be still maintained at 1.0, and scientific research materials are provided for later vaccine research and development.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to an attenuated strain of porcine epidemic diarrhea virus, its cultivation method and application. Background technique [0002] Porcine epidemic diarrhea (Porcine epidemic diarrhea, PED) is caused by porcine epidemic diarrhea virus (porcine epidemic diarrhea virus, PEDV), an acute, highly contagious disease characterized by vomiting, diarrhea, and dehydration in suckling piglets . Pigs of different ages, sexes and breeds are susceptible, but suckling piglets are most at risk. [0003] PED was first reported in the UK in 1971, and then Japan, South Korea, Switzerland, Germany, Belgium, Canada and other countries successively reported the disease. The disease was first reported in China in 1976. At present, it has occurred in 26 provinces, municipalities and autonomous regions in the country. Especially since 2010, the large-scale epidemic in the whole cou...

AI Technical Summary

Problems solved by technology

Differences in the S gene of PEDV epidemic strains and vaccine strains may be the reason for the poor immune effect based on the CV777 vaccine strain

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