Amplification primer, application and detection method for detecting hcv 2a subtype NS5A drug resistance mutation gene

An amplification primer and drug resistance mutation technology, applied in the field of molecular biology, can solve problems such as reducing inhibition effect, and achieve the effects of high effectiveness, good reproducibility and high sensitivity

Active Publication Date: 2019-03-15
黑龙江金域医学检验实验室有限公司
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Problems solved by technology

Direct anti-HCV agents (DAAs) are mainly inhibitors of HCV viral non-structural proteins (NS3, NS5A, NS5B), and certain mutations in HCV genes can significantly reduce this inhibitory effect

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  • Amplification primer, application and detection method for detecting hcv 2a subtype NS5A drug resistance mutation gene
  • Amplification primer, application and detection method for detecting hcv 2a subtype NS5A drug resistance mutation gene
  • Amplification primer, application and detection method for detecting hcv 2a subtype NS5A drug resistance mutation gene

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Embodiment Construction

[0032] In order to facilitate the understanding of the present invention, the present invention will be described more fully below with reference to the associated drawings. Preferred embodiments of the invention are shown in the accompanying drawings. However, the present invention can be embodied in many different forms and is not limited to the embodiments described herein. On the contrary, these embodiments are provided to make the understanding of the disclosure of the present invention more thorough and comprehensive.

[0033] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of ...

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Abstract

The invention discloses an amplification primer for detecting a drug-resistant mutant gene of HCV (hepatitis c virus) 2a subtype NS5A, application and a detection method. The amplification primer fordetecting a drug-resistant mutant gene of HCV 2a subtype NS5A comprises a first amplification primer pair, wherein sequences of an upstream primer and a downstream primer in the first amplification primer pair are shown by SEQ ID NO:1 and SEQ ID NO:2 respectively. The amplification primer for detecting a drug-resistant mutant gene of HCV 2a subtype NS5A can be used for analyzing and studying the mutation condition of the HCV gene so as to provide reference for clinical study on drug resistance. When the amplification primer is adopted to perform detection and analysis such as PCR amplification, the effectiveness is high, the detection success rate can reach 100%, the reproducibility is high, and particularly, the minimum detection limit is low, thus the detection of a sample with low HCV RNA concentration is facilitated while the sensitivity is high.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to an amplification primer for detecting HCV 2a subtype NS5A drug resistance mutation gene, an application and a detection method. Background technique [0002] Hepatitis C is caused by hepatitis C virus (HCV) infection and is mainly transmitted by blood / body fluids. The World Health Organization estimates that 170 million people are infected with HCV worldwide. The anti-HCV positive rate of healthy people in my country is 0.7% to 3.1%, about 38 million people. Due to various factors such as the biological characteristics of the virus and the immune function of the host, it is often difficult for the body's immunity to effectively clear the virus, resulting in about 50% to 80% of HCV-infected patients developing chronic hepatitis, of which 20% to 30% will develop into liver cirrhosis. Every year, 1% to 4% of patients with liver cirrhosis develop hepatocellular carcinoma. Direct a...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12Q1/6869C12N15/11C12R1/93
CPCC12Q1/686C12Q1/6869C12Q1/707C12Q2600/106C12Q2600/156C12Q2521/107C12Q2535/101
Inventor 冯宇鹏郭晓磊曾征宇徐艳艳张鹏博柳盛伟郝必喜
Owner 黑龙江金域医学检验实验室有限公司
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