Nucleic acid membrane strips and kits for Candida species identification and gene mutation detection

Abstract

The invention relates to a diagnostic reagent of candida and particularly relates to a nucleic acid film strip and a kit capable of simultaneously performing identification of candida species and detection of gene mutation. The nucleic acid film strip for identification of candida species and detection of gene mutation comprises a substrate and nucleic acid probes immobilized on the substrate, wherein the nucleic acid probes comprise a nucleic acid probe used for identification of candida species and having base sequences shown in SEQ ID NO: 1-7, a nucleic acid probe used for detection of gene mutation of candida albicans and having base sequences shown in SEQ ID NO: 8-23 and an internal reference nucleic acid probe having a base sequence shown in SEQ ID NO: 24. The nucleic acid film strip and the kit are used for identification of the species and detection of drug-resistant mutant genes according to the gene sequences of candida, judging from molecular structure, results are accurate and reliable and the detection sensitivity is high; the detection time is short and the detection can be completed only just in eight hours.

Description

technical field [0001] The invention relates to a diagnostic reagent for candida, in particular to a nucleic acid membrane strip and a kit that can be used for candida strain identification and gene mutation detection. Background technique [0002] Vaginal candidiasis, also known as vulvovaginal candidiasis (VVC), is a common disease in obstetrics and gynecology. It is a kind of reproductive system infection that is common in women of childbearing age in all age groups. 75% of women have at least one infection in their lifetime. Suffered from fungal vaginitis once. [0003] Candida albicans (C.albicans) is the most common pathogen of candida vaginitis, accounting for about 70-90%. The same host can carry multiple kinds of Candida, and the same Candida of different genotypes can colonize the same or different parts of the host. In recent years, with the widespread use of antibiotics, the spectrum of pathogenic bacteria in candidal vaginitis has also changed. Cases of candi...

AI Technical Summary

Problems solved by technology

Defects of the above detection methods: the positive rate of direct microscopic examination or smear staining method is low, below 80%, which cannot meet the clinical requirements; at the same time, the above three methods cannot be used for bacterial identification, and cannot distinguish different Candida species the goal of

[0010] At present, there is no product that can simultaneously identify Candida strains and detect drug-resistant mutation genes of Candida albicans

At the same time, the existing Candida drug susceptibility and identification technology is still based on traditional fungal isolation culture and drug susceptibility test. During the cultivation process, the requirements for dispensing operation technology are high, and the environment requirements for fungal culture are high. A little careless operation may easily cause fungal infection. It is judged as a false negative due to death; and the culture time is long (about a week), which cannot meet the needs of rapid detection

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