Concentrated adipose-derived stem cell culture supernatant preparation and preparation method and application thereof

A technology of adipose stem cells and culture supernatant, which is applied in the field of concentrated adipose stem cell culture supernatant preparations, can solve the problems of unreasonable methods of repairing scald wounds, concurrent infection, limited use, etc., to achieve efficient repair, strong secretion ability, Apply handy effects

Inactive Publication Date: 2018-02-23
成都远山博桥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, adverse reactions that may be caused by washing with running water to cool down include: concurrent infection caused by bacteria in running water, wound cracking or skin shrinkage due to the effect of running water, scars left after healing, shock caused by excessive body temperature drop, etc.
[0005] Existing medical methods also try to use stem cells to repair wounds, but the existing treatment method needs to maintain the normal growth of stem cells, so it often needs to be configured on the spot, it is difficult to guarantee the quality, and the use is very limited
[0006] In summary, the existing repair methods for burn wounds are unreasonable, have many adverse reactions, cumbersome operations, and limited application

Method used

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  • Concentrated adipose-derived stem cell culture supernatant preparation and preparation method and application thereof
  • Concentrated adipose-derived stem cell culture supernatant preparation and preparation method and application thereof
  • Concentrated adipose-derived stem cell culture supernatant preparation and preparation method and application thereof

Examples

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Embodiment 1

[0032] This embodiment provides a concentrated adipose stem cell culture supernatant preparation, which is mainly obtained by concentrating the supernatant of a cell culture medium cultured from adipose tissue-derived mesenchymal stem cells. The specific preparation method of the concentrated adipose stem cell culture supernatant preparation of this embodiment is as follows: take 40 ml of adipose tissue (the adipose tissue is derived from animals, preferably from humans), and add collagenase type I at a concentration of 0.1% to 0.5% to digest for 30 to 30 ml. After washing with normal saline for 60 min, centrifuge at 2000 rpm / min for 5-10 min, take the precipitated cells, wash and resuspend with normal saline, filter with a filter membrane with a diameter of 70 μm, and obtain adipose-derived stem cells by adding GMP-standard hrEGF, VEGF and bFGF (concentrations of 0.08-0.15 μg / mL, 0.05-0.11 μg / mL and 0.01-0.10 μg / mL respectively) were cultured in serum-free medium for adipose-d...

Embodiment 2

[0034] This embodiment provides a concentrated adipose stem cell culture supernatant preparation, the difference from Example 1 is that in the final step of the concentrated adipose stem cell culture supernatant preparation in this embodiment, the step of adding cytokines hrEGF, VEGF and bFGF is removed .

[0035]The specific steps of this embodiment are as follows: take 40ml of adipose tissue, add 0.1%-0.5% type I collagenase to digest for 30-60min, wash with normal saline, centrifuge at 2000rpm / min for 5-10min, take Cells were precipitated, washed and resuspended with normal saline, and filtered with a filter membrane with a diameter of 70 μm to obtain adipose-derived stem cells. 0.01~0.10μg / mL) of adipose-derived stem cell serum-free medium for culturing; the adipose-derived stem cell serum-free medium in this embodiment was cultured in CO 2 The culture is carried out in an incubator with a temperature of 37° C. in a humid sterile gas environment with a content of 5%. The...

Embodiment 3

[0037] This embodiment provides a concentrated adipose stem cell culture supernatant preparation, the difference from Example 1 is that the GMP standard hrEGF, VEGF and bFGF added at the end of the finished product preparation are different from Example 1. Present embodiment 3 is divided into two examples, respectively as follows:

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Abstract

The invention belongs to the technical field of medical treatment and health and particularly relates to a concentrated adipose-derived stem cell culture supernatant preparation to treat skin scalding. The concentrated adipose-derived stem cell culture supernatant preparation is prepared with supernatant of a cell culture fluid cultured via adipose-derived mesenchymal stem cells; cell factors hrEGF, VEGF and bFGF are added to the preparation. The invention also provides a preparation method and application of the concentrated adipose-derived stem cell culture supernatant preparation. The concentrated adipose-derived stem cell culture supernatant preparation of the invention can significantly promote healing of scald wounds, and the results lie in the aspect: after the use of the preparation, epidermis and dermis can heal and restore their functionality faster than those treated in a positive control and other embodiment groups. In addition, the concentrated adipose-derived stem cell culture supernatant preparation of the invention is free of living cell ingredients, no ethic issues or immune safety issues occur, compounding the preparation when it is needed is not required, and thepreparation is convenient to apply.

Description

technical field [0001] The invention belongs to the field of medical and health technology, and in particular relates to a concentrated fat stem cell culture supernatant preparation, its preparation method and application. Background technique [0002] Tissue injuries caused by physical factors such as flames, hydrothermal fluids, and electricity, and chemical substances such as strong acids and alkalis are collectively referred to as burns and scalds. It is a common disease in daily life and work. Severe burns can cause deep tissue necrosis. If not handled properly, severe burns will fester and fail to heal for a long time. [0003] The healing process of scald wound mainly includes the phase of inflammatory response, cell proliferation and structural remodeling. After tissue injury, fibroblasts and capillary endothelial cells proliferate gradually after inflammatory exudation. With the formation of granulation tissue, the new epithelium advances to the center of the wound...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K38/18A61P17/02
CPCA61K35/28A61K38/1808A61K38/1825A61K2300/00
Inventor 杨远徐子林袁祥于洪涛李巍叶飞轮
Owner 成都远山博桥生物科技有限公司
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