Construction methods and application of Corynebacterium glutamicum recombinant strains for producing L-rare sugars

Abstract

The invention discloses construction methods and an application of Corynebacterium glutamicum recombinant strains for producing L-rare sugars, provides methods for synthesizing the L-rare sugars by using glucose and small-molecular compounds as substrates, and relates to the construction methods of the multiple Corynebacterium glutamicum recombinant strains and the application of the recombinant strains in the fermenting synthesis of the L-rare sugars. The fermenting synthesis of L-fructose, L-tagatose, L-sorbose, and L-psicose by using glucose and glycerol as substrates and the fermenting synthesis of L-rhamnulose, L-fucose, 6-deoxy-L-sorbose and 6-deoxy-L-psicose by using the glucose and 1,2-propylene glycol or L-lactic acid as substrates are achieved. Compared with existing bio-transformation method for synthesizing the L-rare sugar, the above application effectively reduces the production cost of the L-rare sugars; and the obtained L-rare sugars can be applied to the fields of foods, medicines, cosmetics and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a recombinant strain of Corynebacterium glutamicum producing L-rare sugars and a construction method thereof, and its use for fermenting and producing 8 kinds of L-rare sugars. Background technique [0002] Rare sugar is a type of monosaccharide and its derivatives that exist in nature but are very low in content (defined by the International Rare Sugar Society ISRS in 2002). Although the content of rare sugar is very small in nature, it has the advantages of low calorie, high stability, harmonious sweetness, no hygroscopicity, no cariogenicity, and high tolerance, and has attracted wide attention in the fields of diet, health care, and medicine. Concern, they can be used as additives to improve the physical and chemical properties of food, and improve the physiological and health functions of food. In addition, a large number of research results also show that rare sugars play an i...

AI Technical Summary

Problems solved by technology

It has been reported that a multi-enzyme coupling system based on aldolase can be used to synthesize L-fructose, which can avoid the use of coenzyme factors. However, the substrates 3-phosphoglycerol and L-glyceraldehyde are expensive, and it is still difficult to achieve large-scale production (Li Z, Cai L, Qi Q, WangP.Enzymatic synthesis of D-sorbose and D-psicose with aldolase RhaD: Effect of acceptor configuration on enzyme stereoselectivity.Bioorg.Med.Chem.Lett.2011.21.7081-7084), so urgently need to develop A method for efficiently synthesizing L-rare sugars with raw materials as substrates

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