Periosteum material with tissue oxygenation function as well as preparation method and application of periosteum material

A technology of periosteum and tissue, which is applied in the field of polylactic acid-glycolic acid copolymer PLGA particles, periosteum material and its preparation, and periosteum material prepared by electrospinning method. Slow speed and other problems, to achieve the effect of benefiting bone cell growth, simple process and high safety

Active Publication Date: 2018-03-20
SHANGHAI NAT ENG RES CENT FORNANOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the capillaries have not yet formed, the growth of cells on the surface of the artificial prosthesis is slow due to hypoxia, and the slow recovery often directly affects the success of the transplant operation.

Method used

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  • Periosteum material with tissue oxygenation function as well as preparation method and application of periosteum material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of PLGA-coated PFOB particles (PLGA-PFOB)

[0027] Dissolve 5 mg PLGA, 5 mg polylactic-co-glycolic acid-polyethylene glycol (PLGA-PEG), 200 μl Span 80 (S80) / dichloromethane (DCM) (3 / 17, volume ratio) in 200 μl DCM, then mixed with 2 ml of water, and 30 μl / ml of PFOB with a purity greater than 99% was added. Homogenize with a homogenizer for 1 min (ice bath), then sonicate for 3 min, 3 s and 10 s off, at 50% power. Homogenize with a homogenizer for 10 min (homogenize for 1 min and stop for 1 min) and then stir at room temperature for 3 h. Oxygenate the emulsion with medical oxygen for 10 minutes.

[0028] 2. Preparation of electrospinning solution

[0029] Add 0.6 g of poly(racemic lactic acid-caprolactone) copolymer (PDLLA-CL) to 10 ml of solvent dichloromethane: dimethylformamide (DMF) = 7:3 (V / V), stir magnetically at room temperature for 8 Hours; 2 ml of PLGA-PFOB solution was added thereto, and ultrasonication was performed for 10 minutes to obtai...

Embodiment 2

[0034] 1. Preparation of PLGA-coated PFOB particles (PLGA-PFOB)

[0035] Dissolve 1 mg PLGA, 1 mg polylactic-co-glycolic acid-polyethylene glycol (PLGA-PEG), 40 μl Span 80 (S80) / dichloromethane (DCM) (3 / 17, volume ratio) in 40 μl DCM, then mixed with 0.4 ml of water, and 20 μl / ml of PFOB with a purity greater than 99% was added. Homogenize with a homogenizer for 1 min (ice bath), then sonicate for 3 min, 3 s and 10 s off, at 50% power. Homogenize with a homogenizer for 10 min (homogenize for 1 min and stop for 1 min) and then stir at room temperature for 3 h. Oxygenate the emulsion with medical oxygen for 5 minutes.

[0036] 2. Preparation of electrospinning solution

[0037] Add 0.3 g of poly(racemic lactic acid-caprolactone) copolymer (PDLLA-CL) to 5 ml of solvent dichloromethane: dimethylformamide (DMF) = 7:3 (V / V), stir magnetically at room temperature 10 hours; 0.5 ml of PLGA-PFOB solution was added thereto and ultrasonicated for 10 minutes to obtain a spinning soluti...

Embodiment 3

[0041] 1. Preparation of PLGA-coated PFOB particles (PLGA-PFOB)

[0042] Dissolve 5 mg PLGA, 5 mg polylactic-co-glycolic acid-polyethylene glycol (PLGA-PEG), 200 μl Span 80 (S80) / dichloromethane (DCM) (3 / 17, volume ratio) in 200 μl DCM, then mixed with 2 ml of water, and 150 μl / ml of PFOB with a purity greater than 99% was added. Homogenize with a homogenizer for 1 min (ice bath), then sonicate for 3 min, 3 s and 10 s off, at 50% power. Homogenize with a homogenizer for 10 min (homogenize for 1 min and stop for 1 min) and then stir at room temperature for 3 h. Oxygenate the emulsion with medical oxygen for 10 minutes.

[0043] 2. Preparation of electrospinning solution

[0044] Add 0.6 g of poly(racemic lactic acid-caprolactone) copolymer (PDLLA-CL) to 10 ml of solvent dichloromethane: dimethylformamide (DMF) = 7:3 (V / V), stir magnetically at room temperature for 7 Hours; 3 ml of PLGA-PFOB solution was added thereto, and ultrasonication was performed for 10 minutes to obta...

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Abstract

The invention relates to a periosteum material with a tissue oxygenation function as well as a preparation method and an application of the periosteum material. The preparation method comprises the following steps: preparing PLGA (Poly(Lactic-Co-Glycolic Acid)) wrapped PFOB (Perfluorooctyl Bromide) granules, preparing an electrostatic spinning liquid, and preparing a periosteum material. All raw materials used in the periosteum material are approved medical materials in service, and through specific process steps, the periosteum material has a specific functional and is high in security; the periosteum material provided by the invention has an oxygenation function, the problem that an artificial periosteum is insufficient in blood supply in a primary transplanting period relative to a selfperiosteum is solved, essential oxygen is supplied to cell proliferation, and the speed of tissue healing is increased; and the periosteum prepared by using an electrostatic spinning method is of anappropriate pore structure, is beneficial to bone cell growth and bone tissue repairing and is simple in process and easy to transfer.

Description

technical field [0001] The invention relates to a periosteum material with tissue oxygenation function and its preparation method and application, in particular to poly(racemic lactic acid-caprolactone) PDLLA added with perfluoro-n-octyl bromide (PFOB) loaded polylactic acid- Glycolic acid copolymer PLGA particles, periosteal material prepared by electrospinning. The method belongs to the field of biomedical materials, and the prepared materials can be widely used in the repair of periosteum tissue. Background technique [0002] Periosteum is a dense connective tissue membrane that covers almost all bone surfaces except joints. It consists of an outer fibrous layer and an inner cambium, and plays an important role in bone development and fracture healing. The fibrous layer is rich in vascular network, nerves, fibrous cells, collagen and elastic fibers, which makes the bone active and provides the mechanical stability of the periosteum, which is the key to osteogenesis; the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/00A61L27/50A61L27/54A61L27/56D04H1/728D04H1/435D01D5/00
CPCA61L27/00A61L27/18A61L27/50A61L27/54A61L27/56A61L2300/412A61L2430/02D01D5/003D01D5/0092D04H1/435D04H1/728C08L67/04
Inventor 何丹农王萍朱君王杰朱海娥金彩虹
Owner SHANGHAI NAT ENG RES CENT FORNANOTECH
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