Potentilla viscosa Donn extract and application thereof

A kind of extract and technology of Potentilla lentils, applied in the field of sticky Potentilla lentils extract, can solve the problems of unsatisfactory curative effect, few side effects, strong side effects, etc., and achieve good application prospects, significant effects, and improvement of fatty liver and liver damage Effect

Pending Publication Date: 2018-03-23
YANBIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Fatty liver is a chronic metabolic disease, which may require long-term medication. The drugs currently used in clinical practice either have strong side effects or unsatisfactory curative effects. Therefore, there is a clinical demand for drugs for the prevention and treatment of fatty liver with clear curative effects and low side effects. very strong
Therefore, traditional drugs that have fewer side effects and can target multiple targets and multiple pathways may bring new opportunities for treatment

Method used

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  • Potentilla viscosa Donn extract and application thereof
  • Potentilla viscosa Donn extract and application thereof
  • Potentilla viscosa Donn extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Preparation of water extract of Potentilla villosa:

[0035]Cut 2000g of the above-ground part of dried sticky Potentilla into small pieces, put it into a medicine bag, soak it in distilled water for 1 hour, then put the medicine bag into the decoction pot, add distilled water until submerged, tighten the cover symmetrically, turn on the switch, and adjust the temperature to 90°C, close all valves, and decoct for 60 minutes under a pressure of 2000Pa. Extract 2-3 times according to the law. After the end, combine the medicinal liquid, filter it with absorbent cotton, and concentrate it with a rotary evaporator at 80°C and a rotational speed of 75r / min to obtain 1500mL of medicinal liquid. Then use a small rotary evaporator to concentrate the medicinal liquid to an extract. Dry it in an oven at 80°C for 3 hours to obtain 388g of water extract of Potentilla villosa.

Embodiment 2

[0037] 1. Establishment and administration of ICR fatty liver mouse model:

[0038] Fifty 4-week-old male ICR mice (18-20 g) were fed freely and adapted to the environment for a week, and were randomly divided into five groups with 10 mice in each group, which were normal control group (Control, CON), model group (High fatdiet, HF), high-dose group (800mg / kg, HFH) of water extract of Weltia chinensis, low-dose group (400mg / kg, HFL), positive control group (300mg / kg metformin, MET) . The normal control group was fed with 10% kcal feed, and the other groups were fed with 45% kcal high-fat feed for a total of 12 weeks. In the last 3 weeks, the mice were given intragastric administration, once a day, that is, the mice in the normal control group and the model group were fed with 10 % polyethylene glycol solution, the other groups of mice were given corresponding doses of medicine, and the medicine was dissolved in 10% polyethylene glycol solution.

[0039] 2. Determination of in...

Embodiment 3

[0056] Effects of water extract of Potentilla villosa on the expression of related proteins and genes in mouse liver:

[0057] (1) According to the method of step 1 in Example 2, after 12 weeks of feeding and administration, the mice were fasted for 12 hours, and anesthesia (Zoletil 50) was injected intraperitoneally. After complete anesthesia and dissection, the liver was collected and immediately put into liquid nitrogen and stored at -80°C.

[0058] (2) Take tissue pieces of appropriate size and divide them into two groups. One group extracts the total protein in the liver tissue of each group according to the operating instructions of the Pro-Prep protein extraction kit for determining the expression of related proteins; the other group Using the operating instructions of the EASY-BLUE Total RNA Extraction Kit, the total RNA was extracted to determine the expression of related genes.

[0059] (3) For the total protein in each group of liver tissues extracted in step (2), ...

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Abstract

The invention discloses a Potentilla viscosa Donn extract and application thereof. The extract is a water extract of Potentilla viscosa Donn, a 30-100% ethanol extract product or a 30-100% methanol extract. The extract has a remarkable effect of improving high-fat diet induced fatty liver and liver injury and has an obvious effect of reducing blood sugar. It shows that the extract has a remarkableeffect of treating diabetes. There is no report related to therapeutic effect of Potentilla viscosa Donn on fatty liver and diabetes at present. Experimental data show that the high-dose Potentilla viscosa Donn extract group has better hypoglycemic effect than a metformin group and the product shows a good application prospect.

Description

technical field [0001] The present invention relates to the extract of Potentilla villosa, especially water, methanol or ethanol extract of Potentilla villosa and its application in preventing and / or treating liver damage and diabetes. Background technique [0002] Liver injury is a pathological state with outstanding characteristics shared by many liver diseases, which seriously threatens human health. Various harmful factors such as drugs, viruses, alcohol, organisms, etc. may lead to varying degrees of damage to liver function. Liver damage caused by various harmful factors mainly includes drug-induced liver damage, viral liver damage, alcohol-induced liver damage, and immune liver damage. Long-term liver damage often leads to liver fibrosis, which in turn induces liver cirrhosis. An important initiating factor for liver failure and even liver cancer. Therefore, the prevention and treatment of liver cell injury is one of the important links in the clinical treatment of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P1/16A61P3/10
CPCA61K36/73A61K2236/331A61K2236/333A61K2236/39
Inventor 朴光春元海丹李海燕
Owner YANBIAN UNIV
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