Refining technology of betacyanins of suaeda salsa

A technology for betalain and Suaeda salsa, which is applied in the field of preparation of Suaeda salsa betaene, can solve the problems of no betalain and low extraction rate of products, and achieves the advantages of improving extraction rate, improving purity and preventing degradation. Effect

Inactive Publication Date: 2018-03-23
WEIFANG YOURONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies have confirmed that Suaeda salsa is rich in betalain. At present, the extraction rate of the product obtained when extracting betalain in the prior art is low. Due to the instability of betalain, there is almost no pure betalain on the market. Taste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] A kind of Suaeda salsa beet red pigment refining process, comprises the following steps:

[0028] (1) Raw material pretreatment: wash and dry the freshly picked Suaeda salsa stems and leaves, and add an extraction solvent (a mixed solution of malic acid and ethanol of 0.1 ), homogenate, filter, and collect filtrate and precipitate respectively;

[0029] (2) Microwave-assisted enzymolysis once: get the precipitate that step (1) collects, add the composite enzyme a (pectinase, cellulase, papain and α-glucosidase of 1-3% of precipitate weight according to 1 :2:1.5:1 ratio), mix well, adjust pH to 4.8, after microwave treatment (200w, treatment temperature is 35℃, treatment time is 10s, treatment times is 2 times, interval time is 1min), Filter to collect supernatant and precipitate respectively;

[0030] (3) Microwave-assisted secondary enzymolysis: take the precipitate collected in step (2), add compound enzyme b of 1-3% of the weight of the precipitate (complex enzyme ...

example 2

[0035] A kind of Suaeda salsa beet red pigment refining process, comprises the following steps:

[0036] (1) Raw material pretreatment: wash and dry the freshly picked Suaeda salsa stems and leaves, add an extraction solvent (a mixed solution of 0.2M citric acid and ethanol) according to the ratio of solid to liquid 1:8, and mix well Slurry treatment, filtration, and separate collection of filtrate and sediment;

[0037] (2) Microwave-assisted primary enzymolysis: get the precipitate that step (1) collects, add the composite enzyme a (pectinase, cellulase, papain and α-glucosidase according to 2:3 of precipitate weight 1.8%) :2:1.5 ratio), mix well, adjust pH5, after microwave treatment (230w, treatment temperature is 39℃, treatment time is 12s, treatment times is 3 times, interval time is 1.5min), filter, respectively Collect supernatant and precipitate;

[0038] (3) Microwave-assisted secondary enzymolysis: get the precipitate collected in step (2), add compound enzyme b (...

example 3

[0043] A kind of Suaeda salsa beet red pigment refining process, comprises the following steps:

[0044] (1) Raw material pretreatment: wash and dry the freshly picked Suaeda salsa stems and leaves, add an extraction solvent (a mixed solution of 0.4M citric acid and ethanol) according to the ratio of solid to liquid 1:10, and Slurry treatment, filtration, and separate collection of filtrate and sediment;

[0045] (2) Microwave-assisted enzymolysis once: get the precipitate that step (1) collects, add the composite enzyme a (pectinase, cellulase, papain and α-glucosidase of 1-3% of precipitate weight according to 3 :5:5:3 ratio), mix well, adjust pH5.3, after microwave treatment (280w, treatment temperature is 42℃, treatment time is 15s, treatment times is 4 times, interval time is 2min), Filter to collect supernatant and precipitate respectively;

[0046] (3) Microwave-assisted secondary enzymolysis: get the sediment collected in step (2), add the composite enzyme b of sedim...

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PUM

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Abstract

The invention relates to the technical field of extracting of betacyanins, in particular to a refining technology of betacyanins of suaeda salsa. The refining technology comprises the following stepsof pretreating of raw materials, microwave-assisted primary enzyme hydrolysis, microwave-assisted secondary enzyme hydrolysis, ultrasonic extracting, purifying, and drying. The extracting method has the advantage that the extracting rate and purity of the betacyanins product of the suaeda salsa are greatly improved.

Description

technical field [0001] The invention relates to the technical field of betalain extraction, in particular to a method for preparing betalain from Suaeda salina. Background technique [0002] Betalain is a kind of natural plant pigment that widely exists in plants. It is composed of red betalain and betaflavin. The main component of betalain is betalain, which accounts for 75-95% of red pigment. There are isobetaine, former betaine and so on. Betalain, its anticancer, antiviral and antioxidative biological activities make betalain widely concerned in the field of health care products and food additives. [0003] Suaeda salsa is a typical true halophyte, which is widely distributed on saline land such as coastal tidal flats. It is a natural resource with great development potential and has great economic, ecological and social benefits. Current studies have confirmed that Suaeda salsa is rich in betalain. At present, the extraction rate of the product obtained when extractin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 王胜
Owner WEIFANG YOURONG IND
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