Unlock instant, AI-driven research and patent intelligence for your innovation.

Fluorescent quantitative PCR detection method and application for authenticity identification of bezoar

A fluorescence quantitative and detection method technology, applied in the field of medicine, can solve problems such as low production, shortage of resources, adulteration and counterfeiting

Active Publication Date: 2022-03-18
NAT INST FOR FOOD & DRUG CONTROL
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bezoar is widely used in prescription preparations, but resources are scarce, especially natural and cultivated bezoar. The production cycle is long and the output is low. It is a precious and rare medicinal material. Recently, adulteration and counterfeiting have been frequently exposed in the market.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fluorescent quantitative PCR detection method and application for authenticity identification of bezoar
  • Fluorescent quantitative PCR detection method and application for authenticity identification of bezoar
  • Fluorescent quantitative PCR detection method and application for authenticity identification of bezoar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0044]According to one embodiment, the present invention relates to the probe method of the fluorescent quantitative PCR method, including different fluorescent signals or non-fluorescent signal labels (such as FAM, TAMARA, BQ, etc.), different types of probes, etc. Needles (such as TaqMAN probes, MGB probes, etc.).

[0045] In the selection of probes / primers, there are not only screening of reported probes / primers, finding the optimal probe / primers suitable for the purpose of detection (examining specificity and sensitivity), but also self-designed new probes / primers. primers.

[0046] In some embodiments, the probe primers used in the present invention are shown in Table 2.

[0047] Table 2. Probe primers

[0048]

[0049]

[0050] Note: (1) Each set of probe primers includes three sequences, which cannot be split when used simultaneously in the experiment; (2) Sequences 1-3 are used to detect pig-derived components in samples, and sequences 4-6 are used to detect co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a fluorescent quantitative PCR detection probe primer and a detection method for authenticity identification of bezoar, which can be used for authenticity identification of bezoar medicinal materials, prescription preparations and other related products. The present invention also provides the use of probe primers for fluorescent quantitative PCR detection of calculus bezoar authenticity identification, said probe primers are SEQ ID NO: 1‑3, 4‑6, 7‑9, 10‑12, 13‑ 15 and / or the probe primer set shown in 16‑18. The method adopts a fluorescent quantitative PCR probe method to detect the animal-derived components that should exist and the animal-derived components that should not exist in the sample. Applied to the quality control of bezoar medicinal materials, prescription preparations and other related products, the method has strong specificity, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a drug identification and detection method, in particular to a probe primer and a detection method for fluorescent quantitative PCR detection for authenticity identification of bezoar. Background technique [0002] Natural bezoar is the dry gallstones of bovine cattle (Bos taurus domesticus Gmelin), mainly containing bilirubin, bile acids, cholesterol, inorganic elements, protein and amino acids, etc. It is one of the precious Chinese herbal medicines [1-2]. Because its resource is in short supply, the price is expensive, and my country promptly started the research of substitute namely cultivating bezoar, cultivating bezoar in vitro and artificial bezoar in the mid-1950s. [0003] Cultivation of bezoar is the use of live cattle to surgically insert yellowing factors into the gallbladder of cattle to produce bezoar. In vitro cultivation of bezoar is based on the principle and bioche...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2561/113C12Q2563/107C12Q2545/114C12Q2561/101
Inventor 张文娟魏锋马双成
Owner NAT INST FOR FOOD & DRUG CONTROL