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Probe primer used for fluorescent quantitative PCR detection for authenticity identification of calculus bovis, and detection method and application

A fluorescence quantification and probe technology, applied in the field of medicine, can solve the problems of long production cycle, adulteration and counterfeiting, shortage of resources, etc.

Active Publication Date: 2018-04-06
NAT INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Bezoar is widely used in prescription preparations, but resources are scarce, especially natural and cultivated bezoar. The production cycle is long and the output is low. It is a precious and rare medicinal material. Recently, adulteration and counterfeiting have been frequently exposed in the market.

Method used

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  • Probe primer used for fluorescent quantitative PCR detection for authenticity identification of calculus bovis, and detection method and application
  • Probe primer used for fluorescent quantitative PCR detection for authenticity identification of calculus bovis, and detection method and application
  • Probe primer used for fluorescent quantitative PCR detection for authenticity identification of calculus bovis, and detection method and application

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Embodiment approach

[0044]According to one embodiment, the present invention relates to the probe method of the fluorescent quantitative PCR method, including different fluorescent signals or non-fluorescent signal labels (such as FAM, TAMARA, BQ, etc.), different types of probes, etc. Needles (such as TaqMAN probes, MGB probes, etc.).

[0045] In the selection of probes / primers, there are not only screening of reported probes / primers, finding the optimal probe / primers suitable for the purpose of detection (examining specificity and sensitivity), but also self-designed new probes / primers. primers.

[0046] In some embodiments, the probe primers used in the present invention are shown in Table 2.

[0047] Table 2. Probe primers

[0048]

[0049]

[0050] Note: (1) Each set of probe primers includes three sequences, which cannot be split when used simultaneously in the experiment; (2) Sequences 1-3 are used to detect pig-derived components in samples, and sequences 4-6 are used to detect co...

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Abstract

The invention provides a novel probe primer and a detection method for fluorescent quantitative PCR detection for authenticity identification of calculus bovis, which can be used for performing the true and false identification to calculus bovis raw materials, calculus bovis preparations, and other related products. The invention also provides an application of the probe primer used for the fluorescent quantitative PCR detection for authenticity identification of calculus bovis, wherein the probe primer is a probe primer group represented as the SEQ ID No. 1-3, 4-6, 7-9, 10-12, 13-15 and / or 16-18, wherein the SEQ ID No. 13-15 and / or 16-18 are new probe primer groups. The method employs the fluorescent quantitative PCR probe process so as to detect required animal sourced components and unacceptable animal sourced components in a sample. The method is used for quality control for calculus bovis raw materials, calculus bovis preparations and other related products, has strong specificity, high sensitivity and good repeatability.

Description

technical field [0001] The invention belongs to the technical field of medicine, relates to a drug identification and detection method, in particular to a probe primer and a detection method for fluorescent quantitative PCR detection for authenticity identification of bezoar. Background technique [0002] Natural bezoar is the dry gallstones of bovine cattle (Bos taurus domesticus Gmelin), mainly containing bilirubin, bile acids, cholesterol, inorganic elements, protein and amino acids, etc. It is one of the precious Chinese herbal medicines [1-2]. Because its resource is in short supply, the price is expensive, and my country promptly started the research of substitute namely cultivating bezoar, cultivating bezoar in vitro and artificial bezoar in the mid-1950s. [0003] Cultivation of bezoar is the use of live cattle to surgically insert yellowing factors into the gallbladder of cattle to produce bezoar. In vitro cultivation of bezoar is based on the principle and bioche...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2561/113C12Q2563/107C12Q2545/114C12Q2561/101
Inventor 张文娟魏锋马双成
Owner NAT INST FOR FOOD & DRUG CONTROL