Grassland red cattle fat content relevant molecular marker as well as obtaining method and application thereof

A technique for molecular markers and fat content, applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as shortening generation intervals, polymorphisms that have not been reported, and accelerated genetic progress, etc., to achieve maximum use value and The effect of social value

Pending Publication Date: 2018-04-06
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The new strain of Chinese prairie red beef was bred by the beef cattle research team of Jilin Provincial Academy of Agricultural Sciences Animal Husbandry Science Branch after years of selection The research on slaughter traits by breeding experts is not as extensive and in-depth as the research on growth traits, because it is relatively difficult to obtain data on these slaughter traits, especially for large livestock animals, which limits the slaughter traits to a certain extent. Research on genetic markers "However, as people's living standards continue to improve today, people's requirements for beef quality are also getting higher and higher, which requires beef cattle breeding experts to continuously strive to breed better beef cattle breeds to meet people's needs." In view of Considering the above considerations, domestic and foreign beef cattle breeding experts have used marker-assisted selection technology to intensify the research on genetic markers of beef cattle slaughter traits, thus laying the foundation for direct use of genotypes for early selection, shortening generation intervals, and accelerating genetic progress.
[0003] So far, there have been no reports at home and abroad on the polymorphism of the entire gene and its relationship with slaughter traits in the new line population of grassland red beef in China

Method used

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  • Grassland red cattle fat content relevant molecular marker as well as obtaining method and application thereof
  • Grassland red cattle fat content relevant molecular marker as well as obtaining method and application thereof
  • Grassland red cattle fat content relevant molecular marker as well as obtaining method and application thereof

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Effect test

Embodiment 1

[0039] This embodiment provides a method for obtaining molecular markers related to the fat content of prairie red cattle, and the specific steps are as follows:

[0040] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;

[0041] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.

[0042] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;

[0043] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and...

Embodiment 2

[0060] This embodiment provides a method for obtaining molecular markers related to the fat content of prairie red cattle. The specific steps are as follows:

[0061] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;

[0062] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.

[0063] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;

[0064] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs and sur...

Embodiment 3

[0081] This embodiment provides this embodiment to provide a molecular marker related to the fat content of prairie red cattle. The specific steps are as follows:

[0082] Sampling: Randomly select 48 heads of grassland red beef with a new strain, collect blood from the jugular vein, put the blood collected from the jugular vein into a centrifuge tube with EDTA anticoagulant, shake it well and quickly put it in an ice box, and store it at -80°C for later use;

[0083] Grassland red cattle carcass weight, net meat weight, on-site measurement and data acquisition of slaughter rate and net meat rate.

[0084] Slaughter the selected prairie red cattle, measure the carcass weight, net meat weight, and calculate the slaughter rate and net meat rate in accordance with the agricultural industry standard NY / T676-2003 "Beef Quality Classification" of the People's Republic of China;

[0085] 1.1 Carcass weight: refers to the weight without head, skin, tail, hooves, reproductive organs an...

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Abstract

The invention provides a grassland red cattle fat content relevant molecular marker as well as an obtaining method and application thereof, and belongs to the fields of biotechnology and crop and pasture production. The obtaining method of the grassland red cattle fat content relevant molecular marker provided by the invention has the advantages that by a simple and fast experiment method, the accurate grassland red cattle fat content relevant molecular marker can be obtained; the obtained grassland red cattle fat content relevant molecular marker can be applied to grassland red cattle meat fat content identification, and high-quality meat can be well distinguished and screened out, so that the grassland red cattle fat content relevant molecular marker can be better used for guiding the production of crop and pasture, particularly the pasture production; great use value and social value are realized.

Description

technical field [0001] The invention relates to the fields of biotechnology and agricultural and animal husbandry production, in particular to molecular markers related to the fat content of prairie red cattle and their acquisition methods and applications. Background technique [0002] The new strain of Chinese prairie red beef was bred by the beef cattle research team of Jilin Provincial Academy of Agricultural Sciences Animal Husbandry Science Branch after years of selection The research on slaughter traits by breeding experts is not as extensive and in-depth as the research on growth traits, because it is relatively difficult to obtain data on these slaughter traits, especially for large livestock animals, which limits the slaughter traits to a certain extent. Research on genetic markers "However, as people's living standards continue to improve today, people's requirements for beef quality are also getting higher and higher, which requires beef cattle breeding experts t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6827
CPCC12Q1/6827C12Q1/6888C12Q2600/124C12Q2600/156C12Q2523/113C12Q2521/301C12Q2565/501
Inventor 秦立红张国梁李旭王蕾班志斌李海燕赵玉民朱永超张鑫
Owner JILIN ACAD OF AGRI SCI
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