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Method for separation and purification of high-purity dihydromyricetin from ampelopsis plant

A technology for purifying dihydromyricetin and dihydromyricetin, applied in organic chemistry and other fields, can solve the problems of high impurity content in crude extract, time-consuming and labor-intensive, unsuitable for industrial production, etc.

Inactive Publication Date: 2018-04-10
GUIZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The prior art adopts the method of water extraction or organic solvent reflux extraction, combined with recrystallization, column chromatography and other methods to purify, the steps are cumbersome, and the impurity content of the crude extract is relatively high (polysaccharide, protein, etc.), the method of column chromatography The purification method is time-consuming and laborious, and the cost is relatively high; in addition, the product yield is low, which is not suitable for industrial production

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  • Method for separation and purification of high-purity dihydromyricetin from ampelopsis plant
  • Method for separation and purification of high-purity dihydromyricetin from ampelopsis plant
  • Method for separation and purification of high-purity dihydromyricetin from ampelopsis plant

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Embodiment 1

[0028] 1. Extraction of total flavonoids in Ampelopsis-grossedentada (Hand-Mazz) W.T.Wang

[0029] Weigh 500g of dried and coarsely crushed (20-40mesh) grape stem and leaf samples, extract with boiling water twice, add 7500mL of water for the first time, heat and boil for 30 minutes, pour out the decoction, then add about 5000mL of water, Heat and boil for 20 minutes, combine the extracts, filter with a filter cloth while it is hot, leave the filtrate at 0-4°C to precipitate white precipitates, filter with suction, dry and filter the precipitates in a vacuum oven at 45°C to obtain about 150g of total flavonoids (wherein, the crude extract The dihydromyricetin content in the middle is 75.1%).

[0030] 2. Recrystallization and purification of dihydromyricetin

[0031] Weigh 2 g of the total flavonoids obtained from the above-mentioned extraction, dissolve them in 20 mL of absolute ethanol, place them in an organic microporous membrane filter (0.45 um in pore size) for suction f...

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Abstract

A method for separation and purification of high-purity dihydromyricetin from an ampelopsis plant includes the following steps: pulverizing stems and leaves of the ampelopsis plant, extracting by water extraction to obtain a crude extract of dihydromyricetin; and recrystallizing and purifying the crude extract of dihydromyricetin with absolute ethyl alcohol and purified water, to obtain a pure product of dihydromyricetin. According to the method, the crude extract of dihydromyricetin (75.1%) is obtained only by the one-step water extraction method, the crude extract of dihydromyricetin (75.1%)can be purified only by one-time recrystallization to obtain dihydromyricetin with the purity of 98.3%, the purification yield is about 60%, and dihydromyricetin industrialized production becomes possible.

Description

technical field [0001] The invention belongs to the field of natural medicines, and in particular relates to a method for separating and purifying high-purity dihydromyricetin from plants of the genus Vitis genus. Background technique [0002] Snake grape (Amplopsis grossedentata (HandMazz.) W.T.Wang) belongs to the genus of Vitaceae, mainly produced in Fujian, Jiangxi, Hunan, Guangdong, Guizhou, Guizhou and other mountainous hills with an altitude of about 200-1000m. The practice and scientific research of folk tea drinking The results show that snake grapes have antibacterial and anti-inflammatory effects, clearing heat and detoxification, lowering blood sugar and lipids, relieving alcohol and protecting liver, etc., and have anti-cancer, anti-oxidation and other activities. The content of flavonoids in snake grape leaves is quite high, among which (2R,3R)-3,5,7-trihydroxy-2-(3,4,5)-trihydroxyphenyl)chroman-4- Ketone, Dihydromyricetin) dihydromyricetin accounts for about ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/32C07D311/40
CPCC07D311/32C07D311/40
Inventor 郁建平郁浩翔郁建生王恩花何惠
Owner GUIZHOU UNIV