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Low-temperature preservation method of Songjiang perch seminal fluid

A technology for cryopreservation and Songjiang perch, which is applied to the preservation, application, and animal husbandry of human or animal bodies. It can solve problems such as complex methods, low sperm survival rate, and inconvenient use, and achieve the effect of improving the survival rate.

Inactive Publication Date: 2018-04-20
WEIHAI HONGRUN OCEAN TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The current cryopreservation method of Songjiang perch semen generally has the disadvantages of complicated method, inconvenient use, and low survival rate of sperm

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0006] The invention relates to a low-temperature preservation method for the semen of Songjiang perch. When adopting the method to preserve the semen, the semen should be diluted in a glass container of 100 milliliters. The preservation solution described in the method consists of 10 milliliters of sucrose, chlorine Potassium chloride 6 ml, methanol 5 ml, frozen in liquid nitrogen vapor, and glycerol 25 ml added as an additional cryoprotectant. The cryopreservation solution is composed of 0.05-0.5% of sucrose, 0.01-0.15% of potassium chloride, 6-12% of methanol, 0.05-5% of glycerin, and the remaining content is made up by distilled water.

[0007] In summary, the present invention greatly improves the survival rate of sperm, and is easy to use and operate.

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Abstract

The invention belongs to the aquaculture industry and particularly relates to a low-temperature preservation method of a Songjiang perch seminal fluid. The low-temperature preservation method is characterized in that the seminal fluid is diluted at first when the method is adopted to preserve the seminal fluid. A preservation fluid comprises saccharose, potassium chloride and methyl alcohol and isfrozen in liquid nitrogen vapor. Glycerinum is added to serve as an additional cryoprotectant. The frozen preservation fluid comprises the following ingredients by weight percent: 0.05-0.5% of saccharose, 0.01-0.15% of potassium chloride, 6-12% of methyl alcohol, 0.05-5% of glycerinum and the balance of distilled water. To sum up, the survival rate of sperms is greatly improved, and the use and the operation are simple.

Description

Technical field: [0001] The invention belongs to the aquaculture industry, in particular to a low-temperature preservation method for Songjiang perch semen used in the aquaculture industry. Background technique: [0002] The current cryopreservation method of perch semen in Songjiang generally has the disadvantages of complicated method, inconvenient use, and low survival rate of sperm. Invention content: [0003] The purpose of the present invention is to solve the above-mentioned problems, and develop a method for cryopreservation of Songjiang perch semen. [0004] The technical solution to be solved by the present invention is: the present invention relates to a method for cryopreservation of Songjiang perch semen, which is characterized in that: when using this method to preserve semen, the semen should be diluted first, and the preservation solution described in this method consists of , sucrose, potassium chloride, methanol, frozen in liquid nitrogen vapor, and adde...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0284
Inventor 王培亮宋旻鹏谷虹
Owner WEIHAI HONGRUN OCEAN TECH CO LTD