Micro-ecological preparation for biocontrol on tomato anthracnose and preparation method of micro-ecological preparation

A technology for tomato anthracnose and micro-ecological preparations, applied in botany equipment and methods, biocides, biocides, etc., can solve problems such as reducing environmental benefits and increasing environmental pollution, and achieves reducing environmental pollution, improving growth, and nutrition. coordination effect

Inactive Publication Date: 2018-04-20
DALIAN UNIV OF TECH QINGDAO NEW ENERGY MATERIALS TECH RES INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(3) Chemical sterilization and disinfection can quickly reduce the infection of pathogenic microorganisms on tomatoes, but in the process of chemical control, the application of a large number of pesticides will increase environmental pollution and reduce environmental benefits

Method used

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  • Micro-ecological preparation for biocontrol on tomato anthracnose and preparation method of micro-ecological preparation
  • Micro-ecological preparation for biocontrol on tomato anthracnose and preparation method of micro-ecological preparation
  • Micro-ecological preparation for biocontrol on tomato anthracnose and preparation method of micro-ecological preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A microecological preparation for biological control of tomato anthracnose, which is composed of the following raw materials in parts by weight: 100 parts of compound microbial bacterial agent, 4 parts of plant methanol extract, 8 parts of salt solution, 3 parts of glucose, and 0.3 part of polyaspartic acid , 0.1 part of indole acetic acid, 10 parts of fruit juice, 20 parts of water.

[0033] The preparation method of the probiotics of the above-mentioned biocontrol tomato anthracnose, it comprises the following steps:

[0034] (1) After activation of the selected four bacterial groups of Burkholderia vietnamiensis, Brevibacillus brevis, Bacillus subtilis and Streptomyces Cultivate in liquid seed medium at 25°C for 16 hours, then transfer to sterilized fermentation medium at 5% inoculum size, and cultivate at 37°C for 3 days, so that the total number of viable bacteria is ≥2*10 10 cfu / mL.

[0035] The composition of the liquid seed culture medium used by the above-men...

Embodiment 2

[0052] A microecological preparation for biological control of tomato anthracnose, which is composed of the following raw materials in parts by weight: 90 parts of compound microbial bacterial agent, 3.5 parts of plant methanol extract, 10 parts of salt solution, 3 parts of glucose, and 0.2 part of polyaspartic acid , 0.3 parts of indole acetic acid, 12 parts of fruit juice, 25 parts of water.

[0053] The preparation method of the probiotics of the above-mentioned biocontrol tomato anthracnose, it comprises the following steps:

[0054] (1) The selected Burkholderia vietnamiensis, Brevibacillus brevis, Bacillus subtilis, Streptomyces, Lactobacillus acidophilus ) in the five kinds of beneficial microorganisms were respectively activated in the liquid seed medium at 25 ° C for 20 h, and then transferred to the sterilized fermentation medium according to the inoculum size of 5%, and cultivated at 37 ° C for 2 days, so that Total number of viable bacteria ≥ 2*10 10 cfu / mL.

[...

Embodiment 3

[0067] A microecological preparation for biological control of tomato anthracnose, which is composed of the following raw materials in parts by weight: 95 parts of compound microbial bacterial agent, 4 parts of plant methanol extract, 8 parts of salt solution, 3 parts of glucose, and 0.5 part of polyaspartic acid , 0.3 parts of indole acetic acid, 15 parts of fruit juice, 20 parts of water.

[0068] The preparation method of the above-mentioned probiotics for biocontrolling tomato anthracnose comprises the following steps:

[0069] (1) Preparation of complex microbial flora: Four strains of selected Vietnamese Burkholderia (Burkholderiavietnamiensis), Brevibacillus brevis, Bacillus subtilis, and Streptomyces After activation, culture in liquid seed medium at 25°C for 18 hours, then transfer to sterilized fermentation medium at 5% inoculum, and cultivate at 37°C for 2.5 days, so that the total number of viable bacteria is ≥ 2 *10 10 cfu / mL.

[0070] The composition of the li...

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Abstract

The invention relates to a micro-ecological preparation for biocontrol on tomato anthracnose and a preparation method of the micro-ecological preparation and belongs to the technical field of anthracnose prevention and treatment. The micro-ecological preparation provided by the invention comprises the following raw materials in parts by weight: 80-100 parts of a composite microorganism bacterial agent, 3-5 parts of a plant methanol extract, 8-10 parts of a salt solution, 3-4 parts of glucose, 0.2-0.5 part of polyaspartic acid, 0.1-0.3 part of heteroauxin, 10-15 parts of fruit juice and 20-30 parts of water. The preparation method of the micro-ecological preparation comprises the following steps: compounding microorganism colonies, extracting plant methanol, preparing the salt solution, andthe like. By adopting the micro-ecological preparation, a biological prevention and treatment method is adopted, antagonism and inhibition functions of microorganisms are utilized, bacteria are treated and inhibited with bacteria, the richness of microorganism colonies on surfaces of tomato surfaces is maintained, environment pollution caused by chemical pesticides is reduced, the plant growth vigor is improved, and the ecological benefits are increased.

Description

technical field [0001] The invention relates to a micro-ecological agent for biocontrolling tomato anthracnose and a preparation method thereof, belonging to the technical field of anthracnose prevention and control. Background technique [0002] Tomato, also known as tomato, is an annual or perennial herb of the genus Solanaceae. Tomato fruit is rich in nutrients and contains a variety of substances that are beneficial to the human body. Anthracnose, likes high temperature and high humidity conditions, has a wide distribution, complex species, wide host range, and strong transmission ability. It is one of the common diseases of tomato. Generally damage mature fruits, the lesion is round or nearly round on the fruit, and the water-soaked transparent small spot on the diseased part is 5-10mm in diameter, slightly sunken, and black after expanding, with a little concentric ring pattern. Small black spots are densely grown, and when the humidity is high, the lesions secrete a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N65/34A01N65/08A01N43/38A01N59/06A01N59/26A01N59/08A01N59/00A01N59/16A01P3/00
CPCA01N43/38A01N59/00A01N59/06A01N59/08A01N59/16A01N59/26A01N63/00A01N65/00A01N65/08A01N65/34
Inventor 陈兰祝玉洪姜大川
Owner DALIAN UNIV OF TECH QINGDAO NEW ENERGY MATERIALS TECH RES INST CO LTD
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