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Construction method of bionic intestinal model based on micro-fluidic technology

A construction method, microfluidic chip technology, applied in the field of bionic intestinal model construction, to achieve the effects of increasing residence time, increasing fluid contact area, and promoting cell growth

Active Publication Date: 2018-04-24
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0007] At present, there is no related research on the use of peristaltic flow to construct intestinal models. This bionic intestinal chip has great application prospects in biological research and pharmaceutical research and development.

Method used

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  • Construction method of bionic intestinal model based on micro-fluidic technology
  • Construction method of bionic intestinal model based on micro-fluidic technology
  • Construction method of bionic intestinal model based on micro-fluidic technology

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Experimental program
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Embodiment 1

[0029] Construction and Characterization of Biomimetic Intestine Model

[0030] Design and manufacture chips, collagen processing chip channel; intestinal cells adjusted to 1 ~ 5 × 10 6 The cells / mL cell suspension was added to the upper chip channel, and the cells were attached to the porous filter membrane interface to grow. The chip was moved and placed in a 37°C incubator to continue culturing. After 12 hours, the microfluidic driving method was used for continuous perfusion. Set the parameters of the control pump as 9s forward and 3s backward alternately, the flow rate is 1 μL / min, the actual effect is 0.5 μL / min, and the perfusion culture is used as the PF group. The static condition culture simulates Transwell as the control group Staic. Functional characterization of the intestinal model was performed for each group at 5 days of culture.

[0031] The surface structure of intestinal cells in each group was characterized by scanning electron microscopy, and the results...

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Abstract

The invention provides a construction method of a bionic intestinal model based on a micro-fluidic technology, applied to the construction of an in-vitro intestinal model. The micro-fluidic chip prepared by adopting the preparation method is mainly composed of an upper-layer chip, a porous filter membrane and a lower-layer chip, wherein the upper-layer chip is inoculated with intestinal cells, fluid moving forwards and backwards alternatively is applied, peristaltic flow is formed in a micro-channel inoculated with the intestinal cells, the actual fluid condition in the intestinal tract in thebody is simulated by utilizing the peristaltic flow, and a good promotion effect is achieved on the intestinal function. The method has great significance for realizing the intestinal chip of an in-vitro simulation environment, realizing the application of medicine evaluation and the like.

Description

technical field [0001] The invention relates to a construction method of a bionic intestinal model, in particular to a construction method of a bionic intestinal model based on microfluidic technology. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. [0003] Oral administration, as a traditional way of administration, is widely accepted by people because of its relative convenience and safety. The absorption process is one of the key factors determining the bioavailability of orally administered drugs. The small intestine is the main site of oral drug absorption, so at present, various in vitro and in vivo intestinal absorption models are main...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12N5/071
CPCC12M23/16C12N5/0679
Inventor 秦建华李中玉
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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