A rapid expansion method of natural killer cells

A natural killer cell, fast technology, applied in animal cells, vertebrate cells, cell culture active agents, etc., can solve the problems of complex culture process, poor cell effect, and large amount of culture medium used
CN107974431BActive Publication Date: 2020-06-30北京汇智驰康生物科技有限公司
2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
北京汇智驰康生物科技有限公司
Publication Date
2020-06-30

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a rapid amplification method of natural killer cells. The rapid amplification method of the natural killer cells comprises the following steps: A, preparing a culture medium ofthe natural killer cells; B, carrying out isolation and culture of the natural killer cells; B1, removing anticoagulant peripheral blood into a centrifuge tube, performing centrifugation, absorbing upper-layer plasma into another centrifuge tube for later use; B2, diluting the plasma with physiological saline of which the volume is 0.5 to 2 times that of the plasma to obtain diluted blood; B3, separating PBMCs from the diluted blood with Ficoll-Paque; B4, resuspending 3 to 5*10<7> / ml PBMCs in a 40-60 ml culture vessel of a completely activated culture medium, and placing in an incubator of which the temperature is 35 to 40 DEG C and the CO2 concentration is 2 to 8% to be cultured for 2 to 4 days; B5, supplementing a cell expansion culture medium in the completely activated culture medium,and continuously carrying out culture to obtain the natural killer cells. The culture method of the invention can amplify a large number of the natural killer cells in a short time, and is lower in amount of the culture medium, and simple, convenient and safe in culture.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to cell culture technology, in particular to a method for rapidly expanding human natural killer cells. Background technique

[0002] Natural killer cells (natural killer cells, referred to as natural killer cells) were discovered by Herberman et al. Since natural killer cells express many lymphocyte surface markers and are derived from lymphoid progenitor cells in the bone marrow, they are classified as lymphocytes. Natural killer cells belong to a subgroup of white blood cells, which can kill target cells without antigen stimulation, and can exert anti-tumor functions through the functions of immune clearance and immune surveillance.

[0003] The mechanism of natural killer cells to clear tumor cells can be divided into three categories: one is to kill tumor cells by releasing cytotoxic particles; the other is to kill tumor cells by activating the tumor cell apoptosis system through the protein synthesized on the cell surface; ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More