Endoglucanase, its coding gene cel5a-h42 and its application

A cel5a-h42, endoglucanase technology, applied in the field of genetic engineering, can solve problems such as slow process, achieve the effects of short production cycle, high activity, and ensure product specificity
CN107974442BInactive Publication Date: 2019-10-22ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2019-10-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of genetic engineering and in particular relates to endoglucanase as well as an encoding gene cel5A-h42 and application thereof. The gene cel5A-h42 for encoding theendoglucanase has a nucleotide sequence shown as SEQ ID No. 1. Bioinformatic analysis shows that the endoglucanase encoded by the gene belongs to the fifth family of glycoside hydrolase. The inventorsuccessfully clones the encoding gene through a designed primer and the encoding gene is successfully expressed in a prokaryotic expression system; the recombinant endoglucanase obtained by inducing ultrasonic purification has the optimum effect pH (Potential of Hydrogen) of 5.0 and the optimum temperature of 55 DEG C, and can keep relatively stable enzyme activity at 50 DEG C. The enzyme has relatively strong pH tolerance when the pH is 5.0 to 10.0 and 70 percent or more of the enzyme activity can be kept. The recombinant endoglucanase has a relatively great research and industrial application potential.
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Description

technical field

[0001] The invention relates to the field of genetic engineering, in particular to an endoglucanase, its coding gene cel5A-h42 and application thereof. Background technique

[0002] As the main component of plant cell walls, cellulose is currently the most abundant renewable resource in nature, but its stubborn structure limits its utilization. The process of degrading cellulose into glucose requires endoglucanase (Endoglucanase, EC3.2.1.4), exoglucanase (exoglucanase, E.C.3.2.1.91) and β-glucosidase (β-glucosidase, E.C. 3.2.1.21) The three cellulase systems act together. Among them, endoglucanase is the most important component of the cellulase system, it mainly acts on the non-crystalline region of cellulose, randomly hydrolyzes the β-1,4 glycosidic bonds in the cellulose chain, cuts off the long chain of cellulose, It is converted into a large number of short chains with different degrees of polymerization, reducing the degree of polymerization of cellul...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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