Illumination system of light sheet illumination microscope with variable light sheet thickness and length
A lighting system and microscope technology, applied in microscopes, optics, optical components, etc., can solve problems such as changes and inability to apply light sheet fluorescence lighting microscopes, and achieve the effects of eliminating spherical aberration, compact system structure, and uniform light intensity
Active Publication Date: 2021-01-26
SUZHOU UNIV
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Problems solved by technology
Although the beam expansion of the laser is also realized, the total length of the system changes with the beam expansion ratio, so it cannot be applied to light-sheet fluorescence illumination microscopes
Method used
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Embodiment 1
[0040] An illumination system for a light sheet illumination microscope with variable light sheet thickness and length, such as figure 1 As shown, sequentially arranged along the optical axis from the object side to the image side: a laser light source, an afocal zoom beam expander system, a cylindrical lens 5 , and a microscopic objective lens 6 . The wavelength of the laser light source is λ=532nm, and the beam waist radius is 0.5mm. The afocal zoom beam expander system is a four-component mechanically compensated afocal zoom beam expander system of a spherical mirror, and the four-component mechanically compensated afocal zoom beam expander system is a front fixed group 1. Zoom group 2, compensation group 3, and rear fixed group 4. The focal lengths of each component are positive, negative, negative, and positive in turn. The light emitted from the laser source passes through the front fixed group and zoom The group and compensation group are imaged at the front focus of t...
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Abstract
The invention belongs to the technical field of microscopic imaging, and in particular relates to an illumination optical path of a light sheet illumination microscope; it provides an illumination optical path system of a light sheet illumination microscope with variable thickness and length of the optical sheet: from the object side to the image side, sequentially including Axis arrangement: laser light source, four-component mechanical compensation afocal zoom beam expander system of spherical mirror, cylindrical lens, microscopic objective lens; , and then through a cylindrical lens that rotates 90° with the optical axis as the rotation axis to form a sheet of light, and the formed sheet of light is then passed through an aplanatic microscopic objective lens to eliminate due to afocal zoom beam expander system and cylindrical The spherical aberration produced by the lens can make the light intensity of the entire light sheet more uniform, and can realize the observation of biological samples within a certain size range without changing the light sheet illumination system.
Description
technical field [0001] The invention belongs to the technical field of microscopic imaging, and in particular relates to an illumination light path of a light sheet illumination microscope. Background technique [0002] Light sheet illumination microscope is a new type of optical microscope. Different from the traditional fluorescence microscope, the illumination light path of the light sheet illumination microscope and the fluorescence detection light path are perpendicular to each other. The illumination beam passes through the cylindrical lens to form a thin and uniform sheet-like beam within a certain range, while the detection optical path performs detection and imaging in a direction perpendicular to the illumination optical path, and the excitation beam is limited near the focal plane of the detection objective lens. Light sheet illumination microscopy has weak phototoxicity and fast imaging speed. It is suitable for three-dimensional imaging of living tissues and lo...
Claims
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IPC IPC(8): G02B21/00G02B27/09
CPCG02B21/0032G02B27/0966
Inventor 曾春梅姜连马锁冬
Owner SUZHOU UNIV