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Serum miRNA marker combination for screening early breast cancer and application of serum miRNA marker combination

A technology of breast cancer and markers, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems of low throughput, limited sample sources, limited number of candidate molecules, etc.

Inactive Publication Date: 2018-05-08
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of the current experimental methods use traditional qPCR technology to obtain the expression of miRNA in plasma or serum, so the number of candidate molecules detected is limited, the throughput is low, and the source of samples is also very limited

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  • Serum miRNA marker combination for screening early breast cancer and application of serum miRNA marker combination
  • Serum miRNA marker combination for screening early breast cancer and application of serum miRNA marker combination

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Embodiment 1

[0022] 1. High-throughput sequencing data sources:

[0023] Downloaded from the TCGA database (https: / / cancergenome.nih.gov / ) miRNA tissue sequencing datasets of 87 normal subjects and 409 early stage breast cancer patients (stage I or II).

[0024] 2. Serum miRNA marker screening:

[0025] a) Divide the high-throughput data into a normal data set and an early breast cancer data set, perform differential expression analysis, and select 20 miRNAs with a corrected p-value less than 0.05, an average copy number greater than 100, and a fold change greater than 4;

[0026] b) Combined with the normal human serum sequencing data in the relevant literature, further select 11 miRNAs with lower copy numbers as candidate serum miRNA markers, and then perform a full combination of the selected miRNAs, and calculate the amount of each combination according to the high-throughput sequencing data. specificity and sensitivity;

[0027] c) Considering that the marker combination requires hi...

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Abstract

The invention discloses a serum miRNA marker combination for screening early breast cancer and application of the serum miRNA marker combination for screening the early breast cancer. The serum miRNAmarker combination for the early breast cancer, disclosed by the invention, comprises three miRNAs which are has-miR-21-5p, has-miR-10b-5p, and has-miR-99a-5p respectively. According to the invention,the advantages of biological information technologies are adopted, and the verification of a serum sample is combined on the basis of large-scale high-throughput tissue sequencing data, to quickly screen out the serum miRNA combination which can effectively detect the early breast cancer. The screening method can greatly reduce the cost of data acquisition and shorten the time for screening earlybreast cancer serum markers. The serum marker combination comprising the three miRNAs, provided by the invention, is taken as a detection target, so that the detection cost can be lowered and the early detection rate of a breast cancer patient is improved.

Description

technical field [0001] The invention relates to utilizing the advantages of high-throughput sequencing data bioinformatics analysis technology to screen and obtain miRNA combinations that are related to the onset of breast cancer and can accurately distinguish early breast cancer patients, and use real-time fluorescence quantitative PCR technology to verify miRNA markers in serum samples , especially related to serum miRNA marker combinations for early breast cancer screening and their applications. Background technique [0002] Breast cancer is one of the most common malignant tumors in women around the world. According to statistics, there are 169,000 female breast cancer cases in my country every year, ranking the second most common malignant tumor in women. About 45,000 women die from breast cancer in my country every year. The sixth most common malignancy cause of death. Most breast cancers are asymptomatic in the early stage, and most breast cancer patients are not dia...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6869C12N15/11
CPCC12Q1/6869C12Q1/6886C12Q2600/178C12Q2535/122
Inventor 杜红丽庾晓康梁锦升
Owner SOUTH CHINA UNIV OF TECH
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