A light sheet illumination microsection imaging system and imaging result processing method

Abstract

Provided are a light-sheet illumination microsection imaging system and an imaging result processing method. The light-sheet illumination microsection imaging system comprises a light-sheet illumination imaging device, a sample surface cutting device, a sample moving device (600), and an imaging result processing device (700). The light-sheet illumination imaging device performs light-sheet illumination imaging on the surface of a sample (800) by means of oblique incident illumination and oblique detection. The sample surface cutting device is used to cut a sample (800) that has already undergone imaging in order to expose a portion of the sample that has not undergone imaging. The sample moving device (600) is used to control movement of the sample (800), such that different portions of the sample (800) are cut and undergo imaging. The imaging result processing device (700) is used to control coordinated operation of the other devices, and performance of real-time processing on raw imaging data. The imaging result processing method comprises reconstructing intra-layer data and registering inter-layer data in order to obtain an image along a placement direction of the sample (800). The imaging system and imaging result processing method achieve high resolution imaging, especially high axial resolution imaging of large volume samples, while also providing high imaging quality and imaging speed.

Description

technical field [0001] The invention belongs to the field of biological imaging, and more specifically relates to a micro-slice imaging system and imaging result processing method. Background technique [0002] In life science research, it is usually necessary to obtain fine three-dimensional structural information of biological samples in a large volume range. As an important tool, various optical microscopic imaging systems have developed rapidly. Analytical imaging systems are widely used. [0003] figure 1 Shown is a schematic diagram of an existing light-sheet illumination microscope imaging system. Such as figure 1 As shown, the light sheet illumination microscopic imaging system includes an illumination optical path part 101, a detection optical path part 103, and a sample moving part 105; the illumination optical path part 101 forms a light sheet to illuminate the sample, and excites the sample to generate a fluorescent signal; the fluorescent signal passes throug...

AI Technical Summary

Problems solved by technology

[0006] Aiming at the defects and improvement needs of the prior art, the present invention proposes a light sheet illumination microsection imaging system and an imaging result processing method, aiming to solve the problem that the existing optical microscopic imaging system cannot obtain large-volume biological samples. Problems with high resolution, especially high axial resolution

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