Biological combined preparation for cartilage repair and application of biological combined preparation

A kind of cartilage repair and biological technology, applied in the direction of drug combination, medical preparations containing active ingredients, anti-inflammatory agents, etc., can solve the problems that have not been widely used, limit clinical application, low biological activity, etc., and achieve implantation and Good growth effect, avoid loss of cell activity, long-lasting effect

Inactive Publication Date: 2018-05-18
金塞迩医院(广州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the selection of seed cells, adipose stem cells, mesenchymal stem cells, and embryonic stem cells have been proven to promote cartilage differentiation, but they have not been widely used due to issues such as material selection, culture techniques, and ethics.
Autologous cartilage transplantation has both advantages and disadvantages. The advantage is that there is no immune rejection, and the disadvantage is that it is poorly differentiated and biologically active, and the acquisition of chondrocytes is an invasive operation, which is easy to cause complications such as infection and postoperative pain. It is mainly used for small and medium-sized areas. cartilage damage
On the contrary, the source of allogeneic periosteum transplantation is sufficient, which can repair a large area of ​​cartilage damage, but it is prone to immune rejection
Both approaches have limited clinical application

Method used

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  • Biological combined preparation for cartilage repair and application of biological combined preparation
  • Biological combined preparation for cartilage repair and application of biological combined preparation

Examples

Experimental program
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Effect test

preparation example Construction

[0038] 2. Preparation of thermosensitive chitosan hydrogel

[0039] Adjust the pH of 2% chitosan chloride solution to 6.5, the pH of 11.5% sodium β-glycerophosphate (GP) solution to 8.0, and the pH of 2.5% hydroxyethyl cellulose (HEC) to 8.0. Mix the above three solutions into a liquid state (pH=7.2) at room temperature according to the volume ratio of 8:2:2.5, then inject it into a suitable mold, and place it in a 37°C incubator within 10 minutes to condense into a gel.

Embodiment 1

[0041] This embodiment is a biological combination preparation for cartilage repair, which includes a physiological saline suspension of urine regeneration cells, hydrogel and ozone at a volume ratio of 10:5:5; wherein, the urine regeneration cells In normal saline suspension, the content of urine regenerative cells is 3×10 7 individual / mL; the hydrogel is a thermosensitive chitosan-based hydrogel.

[0042] The preparation method of the above-mentioned biological combination preparation for cartilage repair is as follows:

[0043] Take 10mL with a density of 3×10 7 The normal saline suspension of urine regeneration cells per mL was added to a 50mL sterile test tube, and then 5mL chitosan hydrogel was drawn with a sterile syringe, added to the sterile test tube, and suspended with the normal saline solution of urine regeneration cells Mix the solution to obtain a biological composition; before treating the patient, use the syringe to draw 5mL of ozone into a sterile test tube...

Embodiment 2

[0045] This embodiment is a biological combination preparation for cartilage repair, which includes a physiological saline suspension of urine regeneration cells, hydrogel and ozone in a volume ratio of 10:10:10; wherein, the urine regeneration cells In normal saline suspension, the content of urine regenerative cells is 1×10 7 individual / mL; the hydrogel is chitosan grafted with a hydroxyl-containing polymer.

[0046] The preparation method of the above-mentioned biological combination preparation for cartilage repair is similar to Example 1.

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Abstract

The invention relates to a biological combined preparation for cartilage repair and an application of the biological combined preparation. The biological combined preparation is prepared from a normalsaline suspension of urine regenerative cells and hydrogel in a volume ratio being (10-15):(1-10), wherein content of the urine regenerative cells in the normal saline suspension of urine regenerative cells is 1*10<7>-5*10<7>/mL; the hydrogel is chitosan-based hydrogel. The biological combined preparation can be applicable to the cartilage repair with a tissue engineering technology, has good repair effect on cartilage injury, is time-saving in treatment and takes effect quickly.

Description

technical field [0001] The present invention relates to biological preparations, in particular to biological combination preparations for cartilage repair and applications thereof. Background technique [0002] Articular cartilage damage is the key factor causing joint pain or discomfort. Because cartilage tissue has no blood supply and its self-healing ability is very limited, it leads to progressive aggravation of pain and the occurrence of early arthritis. [0003] At present, there are many methods for the treatment of articular cartilage injury, which can be divided into non-cartilage repair and cartilage repair according to the purpose. Among the non-cartilage repair treatment methods, for patients with mild symptoms, there are oral glucosamine; intra-articular injection of sodium hyaluronate and glucocorticoid drugs; infrared therapy and other treatment methods. All can achieve the purpose of relieving pain and delaying degenerative lesions, but these methods have no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/22A61P19/04A61P29/00A61L27/38A61L27/20A61L27/50A61L27/54A61L27/58C08J3/075C08L5/08C08L1/28A61K33/00A61K31/722
Inventor 任生
Owner 金塞迩医院(广州)有限公司
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