Biological combined preparation for cartilage repair and application of biological combined preparation
A kind of cartilage repair and biological technology, applied in the direction of drug combination, medical preparations containing active ingredients, anti-inflammatory agents, etc., can solve the problems that have not been widely used, limit clinical application, low biological activity, etc., and achieve implantation and Good growth effect, avoid loss of cell activity, long-lasting effect
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[0038] 2. Preparation of thermosensitive chitosan hydrogel
[0039] Adjust the pH of 2% chitosan chloride solution to 6.5, the pH of 11.5% sodium β-glycerophosphate (GP) solution to 8.0, and the pH of 2.5% hydroxyethyl cellulose (HEC) to 8.0. Mix the above three solutions into a liquid state (pH=7.2) at room temperature according to the volume ratio of 8:2:2.5, then inject it into a suitable mold, and place it in a 37°C incubator within 10 minutes to condense into a gel.
Embodiment 1
[0041] This embodiment is a biological combination preparation for cartilage repair, which includes a physiological saline suspension of urine regeneration cells, hydrogel and ozone at a volume ratio of 10:5:5; wherein, the urine regeneration cells In normal saline suspension, the content of urine regenerative cells is 3×10 7 individual / mL; the hydrogel is a thermosensitive chitosan-based hydrogel.
[0042] The preparation method of the above-mentioned biological combination preparation for cartilage repair is as follows:
[0043] Take 10mL with a density of 3×10 7 The normal saline suspension of urine regeneration cells per mL was added to a 50mL sterile test tube, and then 5mL chitosan hydrogel was drawn with a sterile syringe, added to the sterile test tube, and suspended with the normal saline solution of urine regeneration cells Mix the solution to obtain a biological composition; before treating the patient, use the syringe to draw 5mL of ozone into a sterile test tube...
Embodiment 2
[0045] This embodiment is a biological combination preparation for cartilage repair, which includes a physiological saline suspension of urine regeneration cells, hydrogel and ozone in a volume ratio of 10:10:10; wherein, the urine regeneration cells In normal saline suspension, the content of urine regenerative cells is 1×10 7 individual / mL; the hydrogel is chitosan grafted with a hydroxyl-containing polymer.
[0046] The preparation method of the above-mentioned biological combination preparation for cartilage repair is similar to Example 1.
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