Medium for magnaporthe grisea culture and spore production and preparation method thereof

A rice blast fungus and culture medium technology, applied in the field of microbial culture, can solve the problems of poor rice blast fungus culture effect, low sporulation efficiency and the like

Active Publication Date: 2018-05-22
INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the technical defects of the prior art, and provides a culture medium and preparation method thereof for cultivating and sporulating the rice blast fungus, so as to solve the poor cultivation effect of the conventional culture medium on the rice blast fungus in the prior art , The technical problem of low sporulation efficiency

Method used

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  • Medium for magnaporthe grisea culture and spore production and preparation method thereof
  • Medium for magnaporthe grisea culture and spore production and preparation method thereof
  • Medium for magnaporthe grisea culture and spore production and preparation method thereof

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Embodiment Construction

[0018] Specific embodiments of the present invention will be described in detail below. In order to avoid too many unnecessary details, well-known structures or functions will not be described in detail in the following embodiments. Unless defined otherwise, technical and scientific terms used in the following examples have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0019] 1. Materials and methods

[0020] 1.1 Strains

[0021] Magnaporthe grisea 1-10 (ZB13), preserved by the Key Laboratory of Pest Control, Jiangxi Academy of Agricultural Sciences.

[0022] 1.2 Test medium

[0023] Rice straw extract: 30g of rice straw is chopped and soaked in 1000mL of water for 16-24h;

[0024] Potato extract: 200g of potatoes, peeled and chopped, soaked in 1000mL of water for 2 hours;

[0025] Rice straw corn flour medium: rice straw 30g, corn flour 20g, agar 18g, water 1000mL;

[0026] PDA medium: potatoes 200g, gluco...

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Abstract

The invention provides a medium for magnaporthe grisea culture and spore production and a preparation method thereof. By an experimental method, the technical solution innovatively designs the ingredients of the medium, and investigates the influence of a variety of compounding ingredients on the growth of magnaporthe grisea with growth rate, produced spore quantity and the like as bases. An experimental result indicates that the medium which is prepared by mixing rice straw extract and PDA medium according to the same volume has a remarkable growth-promoting effect on magnaporthe grisea, andmoreover, the efficiency of spore production is also significantly increased. The medium disclosed by the invention has a good culture effect on magnaporthe grisea, the quantity of produced spores isoutstanding, furthermore, the materials are easy to obtain, the medium is easy to prepare, and the application effect is good.

Description

technical field [0001] The invention relates to the technical field of microbial culture, in particular to a culture medium for cultivating and producing spores of blast fungus and a preparation method thereof. Background technique [0002] Rice blast is the primary disease of rice, and its pathogenic microorganism is Phyricularia grisea, which belongs to Deuteromycetes, Hyphosporium; its sexual generation is called Magnaporthe grisea. In the research of disease resistance of rice varieties and the identification of physiological races of Magnaporthe grisea, the cultivation of strains and the acquisition of conidia are necessary technical links, so the cultivation effect and sporulation efficiency of Magnaporthe grisea have important implications for experimental work. direct impact. [0003] In the prior art, the medium used for the cultivation and sporulation of blast fungus is generally obtained by directly mixing sorghum, rice straw, barley, wheat and other components. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 兰波杨迎青陈洪凡孙强陈建吴斯骏徐沛东李湘民
Owner INST OF PLANT PROTECTION JIANGXI ACAD OF AGRI SCI
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