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Multiplex PCR detection method of skin trait-related gene

A detection method and technology for traits, applied in the field of gene detection, can solve the problems of high cost and high sequence dependence when the number of loci is small

Active Publication Date: 2018-05-22
美因健康科技(北京)有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that the cost is high when the number of sites is small, and the dependence on the sequence near the site is high

Method used

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  • Multiplex PCR detection method of skin trait-related gene
  • Multiplex PCR detection method of skin trait-related gene
  • Multiplex PCR detection method of skin trait-related gene

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Embodiment Construction

[0059] The present invention is explained in detail below.

[0060] Multiplex PCR Primer Design

[0061] Through the comparison of various factors, within an acceptable cost range, multiplex PCR is more suitable for the detection of genes related to skin characteristics at nearly a hundred sites. The design of primers for multiplex PCR is the key and difficult point, which is directly related to the cost and effect of detection. A set of primers with better effect was designed after several experiments, and the primer sequences are shown in Table 1. The selection principle of the primer combinations in Table 1 is designed based on the experience of previous experiments, and then the primers with poor effects are redesigned according to the experimental results. Under this condition, each SNP can reach the specified sequencing depth. If it is a common primer combination, under the specified average sequencing depth, some SNP sites cannot be detected or the sequencing depth i...

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Abstract

The invention provides a multiplex PCR detection method of a skin trait-related gene. The multiplex PCR detection method comprises the following steps: (1) designing a primer combination; (2) after primary PCR amplification, purifying a product through magnetic beads; (3) performing secondary PCR amplification on the product purified in the step (2), and purifying an amplification product throughthe magnetic beads; (4) performing next-generation sequencing on the product purified in the step (3). By the multiplex PCR detection method, the problem that the amplification effects of sites related to skin traits are detected by a multiplex PCR technology is solved, the detection cost can be significantly reduced and the detection effect can be improved.

Description

technical field [0001] The invention relates to gene detection, in particular to a multiplex PCR detection method for genes related to skin traits. Background technique [0002] With the improvement of people's living standards, the demand for skin care products is increasing. In addition to the influence of external factors such as living environment and care level, genes have an important impact on the characteristics of human skin, and even directly determine the characteristics of skin. There are single base mutations (SNP) in some important positions of genes. Different genotypes will be formed after a base mutation at a certain site, and different genotypes often determine different skin characteristics. By detecting your own specific genotype, you can more effectively choose skin care products according to your own characteristics, so that you can perform skin care in a more targeted manner. [0003] There are currently a variety of techniques for the detection of ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/686C12Q1/6869
CPCC12Q1/686C12Q1/6869C12Q1/6888C12Q2600/156C12Q2537/143C12Q2535/122
Inventor 戚珠云肖哲焦少灼禹黎尹朝华
Owner 美因健康科技(北京)有限公司