Tribonema strain as well as culture and applications thereof

A technology of yellow hair algae and algae strains, applied in the direction of single-cell algae, microorganisms, biochemical equipment and methods, etc., can solve the problems of few research records, slow growth speed, slow oil accumulation speed of yellow hair algae, and achieve oil accumulation fast effect

Active Publication Date: 2018-06-01
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to existing reports, there are 28 species in this genus, and there are only 17 species and 2 varieties in my country, and there are few related research records, and it is generally believed that the oil accumulation speed of Xanthophyllum is slow, the growth rate is slow, and the components in the oil have special functions So far, there have been no reports on the large-scale cultivation and industrial application of Xanthophyllum

Method used

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  • Tribonema strain as well as culture and applications thereof
  • Tribonema strain as well as culture and applications thereof
  • Tribonema strain as well as culture and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening and molecular identification of Xanthophyllum ENN177

[0034] 1. Screening of Xanthophylla ENN177 (Tribonema sp.)

[0035] Soil samples were taken in the northeast of Inner Mongolia in autumn. In the laboratory, BG11 medium diluted 1 / 10 was added to the water samples, enriched and cultured for 2-3 days under natural light and room temperature, and the samples were treated with sterile deionized water. Dilute, take a single algae silk and carry out purification culture in a 24-well plate, the medium is BG11 medium, the culture temperature is 25°C, and the light is natural light. The purified algae filaments in the orifice plate were transferred to the Erlenmeyer flask for expansion culture, and finally the pure algae strain of ENN177 was obtained.

[0036] Table 1BG11 medium composition

[0037] Substance name

Dosage per liter of deionized water

NaNO 3 (sodium nitrate)

1.5g

K 2 HPO 4 ·3H 2 O(dipotassium hydrogen...

Embodiment 2

[0060] Indoor cultivation of embodiment 2ENN177 algal strain

[0061] In a column reactor with an inner diameter of 5cm and a height of 60cm, use sterile BG11 medium for cultivation, the cultivation temperature is 25°C, the initial inoculum concentration is about 1g / L, and the mixture of filtered air and carbon dioxide is introduced. Gas, wherein the content of carbon dioxide is 1-2% of the total amount of mixed gas. The initial light intensity is 120umol / m 2 / s, according to the growth of biomass in the later stage, the light intensity is increased to 200umol / m 2 / s. Samples were taken every day to determine the biomass and fatty acid content in the cells, and the cells were cultured for 4 days in total.

[0062] Biomass measurement method: Accurately measure 50mL of algae liquid, filter it with a glass fiber membrane (pore size of 0.45um) with a known initial weight after drying, rinse with 150mL of deionized water three times to remove ash, and then remove the algae cell...

Embodiment 3

[0064] The oil production of embodiment 3ENN177 strain

[0065] Insert the ENN177 algae at the end of the exponential growth stage into a column reactor with an inner diameter of 5 cm and a height of 60 cm. The initial inoculation amount of algal cells is about 1.5 g / L. The medium used is BG11 medium lacking nitrogen and phosphorus. The temperature is 25°C, and the initial light intensity is 150umol / m 2 / s, the subsequent light intensity is gradually increased to 200umol / m 2 / s, other parameters are the same as in Embodiment 2. Samples were taken every day to measure the fatty acid content in the cells, and the biomass was measured every two days for a total of 3 days of culture. The results were as follows: Figure 4 shown.

[0066] Fatty acid composition and content determination method: weigh 25mg of freeze-dried sample, add 2mL 2% H 2 SO 4 Anhydrous methanol-toluene solution. After filling with nitrogen, heat at 80°C for 2 hours. Afterwards, 1 mL of pure water and n...

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Abstract

The invention provides a tribonema strain as well as culture and applications of the tribonema strain. The tribonema is named ENN177 (Tribonema sp.), is preserved in the China General MicrobiologicalCulture Collection Center (CGMCC) on Sep 27, 2017, and is assigned with the accession number of CGMCC No.14717. The tribonema can be applied to the fields including CO2 emission reduction, wastewatertreatment, and production of biofuels, foods, nutrition health care products, medicine products, cosmetics, chemical products, feeds and baits.

Description

technical field [0001] The present invention relates to a strain of Xanthophyllum sp. and its cultivation and application. Specifically, the present invention relates to a strain of Xanthophyllum sp. , pharmaceutical products, cosmetics, chemicals, feed and bait applications. Background technique [0002] While the social economy is developing rapidly, the greenhouse effect and energy crisis are also coming one after another, which are threatening global security. The capture and utilization of carbon dioxide has risen from an environmental issue to an international political issue, and has become the focus of many governments. Various countries have formulated emission reduction targets and promulgated support policies for new energy sources, especially biofuels. Biofuels have gone through three stages of development: the first generation of biofuels is derived from corn, soybeans, sugarcane, sugar beets, vegetable oils, and animal fats, and has reached commercial levels ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12C12N1/125C12R2001/89
Inventor 白雪梅吴洪李青尹顺吉王慧岭王琳杨巧利刘芳伟陈昱朱振旗
Owner ENN SCI & TECH DEV
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