Bacillus strain for degrading aflatoxin b1 and its screening and application

Abstract

The invention discloses a bacillus strain for degrading aflatoxin B1 and its screening and application. The strain is classified as Bacillus aryabhattai DT, and is preserved in China General Microorganism Culture Collection and Management Center with the preservation number CGMCC 14949. The treatment method includes strain screening and isolation and 16S rDNA identification using coumarin as the only carbon source medium; at the same time, the degradation of aflatoxin B1 by the fermentation medium of the bacteria is explored. Pick a single colony of Bacillus aryabhattai from the solid medium and inoculate it in 50 mL of growth medium, shake it on a shaker at 30-40°C for 12-24 hours, and mix the fermentation broth with a concentration of 2 μg / mL aflatoxin B1 was reacted in the dark for 3-4 days, and the degradation rate could reach 82.98%. The Bacillus ayerbordella screened by the invention has obvious degradation effect on aflatoxin B1, and has the characteristics of high efficiency, energy saving, environment friendliness, etc., and can be applied to the preparation of related aflatoxin detoxification agents.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and particularly relates to a bacillus strain capable of degrading aflatoxin B1 and application thereof. Background technique [0002] Aflatoxin is the most toxic mycotoxin found so far, and its basic structure is difuran ring and coumarin. It is mainly a secondary heterocyclic metabolite produced by Aspergillus flavus and Aspergillus parasiticus. Aflatoxins are highly toxic substances with strong carcinogenicity. Long-term intake of toxins accumulate in the liver can cause liver cancer and cause cancer in other organs such as kidneys, lungs, and gastrointestinal tract. Among all aflatoxins, aflatoxins B1 (AFB1) is the most toxic and has been classified as a Class I human carcinogen by the International Agency for Research on Cancer (IARC). Aflatoxins are mainly found in moldy peanuts, grains, nuts and rice. According to the statistics of the Food and...

AI Technical Summary

Problems solved by technology

Physical methods mainly include light, ultrasonic and ultra-high temperature, etc., but these processes may be affected by factors such as temperature and time, resulting in damage and loss of nutrients in food

The chemical method is to add some substances to degrade or destroy aflatoxins, such as adding oxidants, ammonium solution and sodium hydroxide method, etc., but these chemical substances may remain in food and are difficult to remove, so conventional physical and chemical methods to remove Aflatoxins in food have disadvantages such as unstable effects, large loss of nutrients, poor palatability of feed, and difficulty in large-scale production, so it is difficult to be widely used in production practice

Images