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Method for Determination of Vitamin A and E in Serum by Liquid Chromatography Tandem Mass Spectrometry

A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of determination of vitamin A and E in serum by liquid chromatography and tandem mass spectrometry, which can solve the problems of detection component interference, difficult operation, misjudgment of analytes, etc., and increase detection specificity , convenient operation and implementation, avoiding the effect of potential interference

Active Publication Date: 2020-08-21
PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, in this technical scheme, liquid chromatography is used for analysis, which needs to rely on the separation of different analytes by the chromatographic column with different retention times and different ultraviolet absorption wavelengths for detection, not direct detection of the analyte, specific The sensitivity is low, and the composition of the clinical sample is complex, there may be substances with the same chromatographic retention time and ultraviolet absorption as the analyte, which will interfere with the detection components; while the liquid chromatography tandem mass spectrometry is used for analysis, vitamin A and E are only One ion pair is used for monitoring, but there may be two kinds of analog interferences in nature with the same precursor ion and product ion. Since the patient's serum is measured, the serum components of different patients are different, and the drugs taken are also different. Composition is complex, therefore, monitoring with a single transition may lead to misidentification of the analyte
[0008] Not only that, vitamins A and E are easily oxidized under air and light, and the method also shows that it is necessary to avoid light during the whole process of standard preparation, but it is basically impossible to completely avoid light during operation, and the operation is difficult

Method used

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  • Method for Determination of Vitamin A and E in Serum by Liquid Chromatography Tandem Mass Spectrometry
  • Method for Determination of Vitamin A and E in Serum by Liquid Chromatography Tandem Mass Spectrometry
  • Method for Determination of Vitamin A and E in Serum by Liquid Chromatography Tandem Mass Spectrometry

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Embodiment 1

[0047] 1. Prepare reagents, including sample preparation solution, standard solution, and instrument analysis solution, as follows:

[0048] 1.1 The sample preparation solution contains: methanol solution, n-hexane, zinc sulfate solution, isotope internal standard, complex solution.

[0049] (1) Methanol solution: a methanol solution containing 30 mg / L 2,6-di-tert-butyl-p-cresol (BHT).

[0050] (2) n-hexane: chromatographic grade n-hexane.

[0051] (3) Zinc sulfate solution: an aqueous solution with a zinc sulfate concentration of 0.2M.

[0052] (4) Isotopic internal standard:

[0053] Retinol-d6 solution: the concentration is 0.8μg / L;

[0054] α-tocopherol-d6 solution: the concentration is 15μg / L.

[0055] (5) Complex solution: methanol: water = 8:1.

[0056] 1.2 Standard solution: use Cerilliant’s accurate definite value standard solution as the mother solution of the standard solution, mix the mother solution of vitamin A and the mother solution of vitamin E, use a con...

Embodiment 2

[0075] The difference between this embodiment and embodiment 1 is:

[0076] Mobile phase A: methanol solution containing 0.1% formic acid, mobile phase B: aqueous solution containing 0.1% formic acid.

Embodiment 3

[0078] The difference between this embodiment and embodiment 1 is:

[0079] Mass spectrometry selects multiple ion monitoring reaction mode, positive ion mode, electrospray ionization, ion source temperature 300 ℃, GS1:80, GS2:80.

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Abstract

The invention belongs to the technical field of determination of vitamins and specifically discloses a method for determining a vitamin A and a vitamin E in serum through a liquid chromatography-tandem mass spectrometry. According to the method, each to-be-determined object is monitored by adopting two ion pairs, wherein one ion is used for quantitative monitoring, and the other ion is used for qualitative monitoring, so that the specificity of detection is increased, and the potential interference in complex blood components is avoided. Additionally, in the method, by adding an antioxidant into solution, an operation process can be completed without light shielding, and the operation is implemented conveniently.

Description

technical field [0001] The invention belongs to the technical field of vitamin detection, and in particular relates to a method for measuring vitamin A and E in serum by liquid chromatography tandem mass spectrometry. Background technique [0002] Vitamins are a class of organic substances necessary to maintain life activities, and maintain human health by regulating body metabolism. Most vitamins cannot be synthesized by the body or are not synthesized enough to meet the needs of the body and must be obtained through food. A few vitamins can be obtained through self-synthesis, but when the body is in a state of disease or affected by lifestyles, it can also lead to deficiency. Both vitamin A and vitamin E are fat-soluble vitamins and are essential nutrients for the human body. Vitamin A (vitaminA), also known as retinol, is an unsaturated monohydric alcohol with an alicyclic ring. It has important physiological functions such as maintaining vision, maintaining epithelial t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 禹松林邱玲
Owner PEKING UNION MEDICAL COLLEGE HOSPITAL CHINESE ACAD OF MEDICAL SCI
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