Preparation and application of a surface-enhanced Raman sensor for bimolecular recognition of dopamine

A surface-enhanced Raman and dopamine technology, applied in Raman scattering, instruments, scientific instruments, etc., can solve the problems of cumbersome sample processing, large sample volume, poor SERS signal, etc., achieve high sensitivity and selectivity, and simple preparation method. , to avoid the effect of potential interference

Active Publication Date: 2021-01-05
FUJIAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are limited in practical applications, and the coexistence of ascorbic acid (AA), uric acid (UA) and other analogues in the central nervous system makes electrochemical analysis challenging because the oxidation potential of these substances is close to that of dopamine on solid electrodes. Oxidation potential of , resulting in an overlapping response
The latter, chromatographic and fluorescent assays, are limited by tedious sample handling, complex probe synthesis or requirements for expensive instrumentation
Chinese Patent No. (CN 105572200 B) discloses a modified glassy carbon electrode, preparation method and application for detecting dopamine in the presence of ascorbic acid. The detection limit of this method for dopamine is 70.3nmol / L, which cannot meet the requirements of actual biological samples. Detection of dopamine in urine, blood, and cerebrospinal fluid
Chinese Patent No. (CN 104020195B) discloses a double-recognition dopamine imprinted electrochemical sensor and its preparation method and application. The electrochemical sensor designed by this method has excellent specific recognition ability and anti-interference ability for the detection of dopamine, but the imprinted molecular The preparation process is complex and requires a large amount of sample
Dopamine has small scattering cross-section, low Raman activity, and poor signal for direct detection of SERS, so it is essential to develop a simple and rapid method to detect dopamine in complex biological samples

Method used

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  • Preparation and application of a surface-enhanced Raman sensor for bimolecular recognition of dopamine
  • Preparation and application of a surface-enhanced Raman sensor for bimolecular recognition of dopamine
  • Preparation and application of a surface-enhanced Raman sensor for bimolecular recognition of dopamine

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Embodiment 1

[0041] Preparation of a surface-enhanced Raman sensor for bimolecular recognition of dopamine

[0042] (1) Preparation of silver-coated glass sheet with Raman enhancement effect by silver mirror reaction

[0043] Put the 1cmx1cm glass slice into ethanol, acetone, deionized water and ultrasonically clean it for 10min respectively. Configuration of silver ammonia solution: add 0.5-1mL of concentrated ammonia water dropwise to 50mL of 2% (mass fraction) silver nitrate solution, and shake while adding dropwise. Spread the glass slides in a petri dish, pour 20mL of silver ammonia solution, shake gently, then add 1-1.5mL of 6% (mass fraction) glucose solution, heat in a water bath for 15min, the temperature of the water bath is 70°C-80°C;

[0044] (2) Preparation of silver-coated glass sheet modified with mercaptopropionic acid

[0045] Drop 100 μL of mercaptopropionic acid (MPA) 5mmol / L on the 1cmx1cm silver-coated glass slide prepared in step (1), incubate at room temperature fo...

Embodiment 2

[0052] Quantitative detection of dopamine

[0053] The bimolecular recognition dopamine surface-enhanced Raman sensor prepared in Example 1 is used to quantitatively detect dopamine, and the concentration of dopamine is as follows: 10 -4 mol / L, 10 -5 mol / L, 10 -6 mol / L, 10 -7 mol / L, 10 -8 mol / L, 10 -9 mol / L, 10 - 10 mol / L, 10 -11 mol / L, 10 -12 mol / L, 10 -13 mol / L; Add it on the silver film modified with mercaptopropionic acid prepared in Example 1 step (2), incubate at room temperature for 45min, wash with distilled water for 5min, and then add dropwise 100 μL of the solution used in Example 1 step (4) after drying. The cubic nano-silver modified with mercaptophenylboronic acid was incubated at room temperature for 40 minutes, washed with distilled water for 5 minutes, and tested by Raman spectroscopy; the spectra were recorded by a Raman spectrometer with an excitation wavelength of 785nm and an excitation power of 2.5mw, and 5 spectra were taken. Plot the average v...

Embodiment 3

[0055] Selective detection of dopamine

[0056] The bimolecular recognition dopamine surface-enhanced Raman sensor prepared in embodiment 1 is used for the specific detection of dopamine, and 10 in embodiment 2 -4 mol / L of dopamine is replaced by 10 -4 mol / L uric acid, tyrosine and glucose. Detect 5 spectra and take the average value, select 1073cm -1 The intensities of the peaks are compared, as Figure 5 As shown, it can be seen that a strong response is obtained only in the presence of dopamine. However, no significant signal was present in the presence of a large excess of xenobiotics, suggesting no significant interaction between the other interferents used in the assay and the recognition molecules (mercaptopropionic acid and mercaptophenylboronic acid). The selectivity is mainly attributed to the unique molecular structure and a kind of phenolic hydroxyl group and amine group, which react with borate and mercaptopropionic acid respectively, indicating that the senso...

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Abstract

The invention discloses preparation and application of a bimolecular dopamine recognition surface enhanced Raman sensor. According to the preparation and application, a surface enhanced Raman scattering (SERS) spectrum method is used for carrying out ultra-sensitive detection on dopamine on the basis of specific recognition from mercaptophenyl boronic acid and mercaptopropionic acid to amido. Thepreparation and application are realized through fixing the mercaptopropionic acid on the surfaces of silver films to capture dopamine and functionalized silver cubic nanoparticles of the mercaptophenyl boronic acid (MPBA) to generate plenty of SERS hotspots. Recognition probe MPBA is directly used as a Raman enhanced signal molecule, and when the method is used for detecting dopamine, the detection limit is 10-13 mol / L. The bimolecular dopamine recognition surface enhanced Raman sensor is not only capable of improving the specific recognition for dopamine in complicated biological samples, but also capable of effectively decreasing background signals.

Description

technical field [0001] The invention relates to the technical field of surface-enhanced Raman analysis and detection, in particular to a preparation method of a surface-enhanced Raman sensor for bimolecular recognition of dopamine and the application of dopamine in human urine, blood, and cerebrospinal fluid. Background technique [0002] Dopamine (DA) is a catecholamine neurotransmitter that plays important roles in the central nervous, vascular and hormonal systems. It is widely distributed in brain tissue and body fluids. Abnormal changes in DA concentration in the body are associated with serious neurological, renal, and cardiac diseases such as schizophrenia, Huntington's disease, Alzheimer's disease, and Parkinson's disease. Accurate and sensitive detection of dopamine in biological samples is very beneficial for understanding the biological functions of dopamine and for the diagnosis and treatment monitoring of dopamine-related clinical diseases. [0003] Electroche...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 陆德婵许慧瑶卢玉栋沈慧英吴阳陈玉婷
Owner FUJIAN NORMAL UNIV
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