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Polystictus glycopeptide active component as well as preparation method and application thereof

A technology of intracellular glycopeptides and active components of Yunzhi, applied in the application of fatty liver or hyperlipidemia, active components of Yunzhi intracellular glycopeptides and its preparation, in the field of treatment of alcoholic liver disease, to improve medical efficacy and Health care value, excellent therapeutic effect, effect of enhancing efficacy

Inactive Publication Date: 2018-06-08
JIANGSU SHENHUA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Propylthiouracil, colchicine, phosphatidylcholine, anti-endotoxin agents, lipid-lowering drugs, silymarin and other drugs have also been used clinically to try to treat alcoholic liver injury, but there are many limitations

Method used

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  • Polystictus glycopeptide active component as well as preparation method and application thereof
  • Polystictus glycopeptide active component as well as preparation method and application thereof
  • Polystictus glycopeptide active component as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] ① Preparation of Yunzhi mycelium:

[0049] Slant activation: take the Yunzhi slant isolated and identified in the laboratory at 4°C, inoculate it on the PDA slant in a sterile ultra-clean bench, incubate at 28°C for 5 days, and observe the growth of the bacteria; primary seed cultivation: the Yunzhi mycelium Blocks were inoculated from the PDA slant into the Yunzhi seed medium, placed in a shaker at 28°C, and cultured at 180rpm for 4 days; secondary seed culture: Inoculate the first-level Yunzhi seeds in 400L at an inoculation amount of 10% (v / v) In a fermenter, cultivate for 4 days under the conditions of temperature 28°C, rotation speed 180rpm, and air flow 1v / v m; fermentation culture: inoculate all Yunzhi seed liquid in a 400L fermenter for 4 days into a 4t fermenter through a pipeline , cultured for 3 days under the conditions of temperature 28°C, rotational speed 180rpm, and air flow 1v / v·m. After the fermentation is finished, the fermentation liquid is centrifug...

Embodiment 2

[0087]Patient Wang, male, 62 years old, suffering from alcoholic liver and fatty liver, was diagnosed by the internal medicine department of a tertiary hospital. Transferase (GGT) 167.8, alkaline phosphatase (ALP) 154.2, triglyceride (TG) 7.0, total cholesterol (TC) 2.5, liver fibrosis 4 items (including laminin (LN) 221.2, hyaluronic acid ( HA) 306.5, type III procollagen (PCⅢ) 200.5, type IV collagen (CⅣ)) 151.7, give the active ingredient of Yunzhi intracellular glycopeptide 5 mg / time in the embodiment of the present invention, bid, take for 2 months, all serum indicators Alanine aminotransferase (ALT) 44.8, aspartate aminotransferase (AST) 47.6, glutamyl transferase (GGT) 67.8, alkaline phosphatase (ALP) 54.2, triglyceride (TG) 5.0, total cholesterol (TC) 1.5, 4 items of liver fibrosis (including laminin (LN) 91.2, hyaluronic acid (HA) 103.5, type III procollagen (PCⅢ) 80.5, type IV collagen (CⅣ)) 85.7, the patient reported taking Yunzhi intracellular glycopeptide After u...

Embodiment 3

[0089] Patient Li, male, aged 67, had drug-induced liver injury due to taking anti-tuberculosis drugs, and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) continued to exceed the normal range for many years; now he has been diagnosed with alcoholic liver disease by the internal medicine department of a tertiary hospital With drug-induced liver injury, the active ingredient of intracellular glycopeptide of Yunzhi according to the embodiment of the present invention was given 4 mg / time, bid, for 3 months. After treatment, the indicators were alanine aminotransferase (ALT) 54.7, aspartate aminotransferase (AST) 48.3, glutamyl transferase (GGT) 77.7, alkaline phosphatase (ALP) 59.2, triglyceride (TG) 5.3, total cholesterol (TC) 1.6, 4 items of hepatic fibrosis (including laminin (LN) 81.2, hyaluronic acid (HA) 113.5, type III procollagen (PCⅢ) 82.5, type IV collagen (CⅣ)) 84.3, the patient reported taking cloud After taking the active ingredient of intracellula...

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Abstract

The invention discloses a polystictus glycopeptide active component as well as a preparation method and application thereof. The polystictus glycopeptide active component comprises the following steps: preparation of coriolus versicolor mycelium; extraction of the polystictus glycopeptide active component from the coriolus versicolor mycelium; separation, purification and enzymatic hydrolysis of the polystictus glycopeptide active component. The active component is found by comparing the therapeutical effect of the polystictus glycopeptide active component on an acute alcoholic liver injury mouse model to better reduce the vitalities of AST and ALT in serum, reduce the contents of Triglycerid, MDA, NEFA, TC and LDL-TC in the serum, improve the content of HDL-TC in the serum and realize thebetter therapeutical effect of resisting alcoholic liver injury. According to the preparation method disclosed by the invention, active functional factors for preventing and treating the alcoholic liver injury are found from natural food resources; the obtained polystictus glycopeptide active component can reduce indicators of the ALT, the AST, cholesterol, free fatty acid and the like in the serum, and has a good application value and a potential market prospect in aspects of preventing and treating alcoholic liver disease, fatty liver or hyperlipidemia.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and in particular relates to an active component of versicolor versicolor intracellular glycopeptide, a preparation method thereof, and an application of using the component to treat alcoholic liver disease, fatty liver or hyperlipidemia. Background technique [0002] Alcoholic liver disease (ALD) is toxic liver damage caused by long-term heavy drinking of alcoholic beverages. With the rapid development of society and the improvement of human material living standards, the global consumption of wine and wine products is increasing year by year, and the incidence of alcoholic liver disease is also gradually increasing. Excessive intake of alcohol has become an important cause of morbidity and death worldwide, and it is also one of the main causes of chronic and acute liver diseases worldwide. Long-term excessive or one-time heavy drinking of alcoholic products can lead to alcoholic liver disease,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P21/06A61K38/01A61P1/16A61P3/06A23L33/18C12R1/645
Inventor 许正宏史劲松龚劲松陆震鸣徐国华王英燕赵伏梅袁峰
Owner JIANGSU SHENHUA PHARMA
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