Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof

Abstract

The invention relates to the technical field of biochemical detection of micro-fluidic chips and in particular relates to a biochemical detection micro-fluidic chip, a biochemical detection micro-fluidic chip system and applications thereof. The chip comprises a sampling unit (1), a reaction unit (3) and a waste liquid unit (4) and a first quantifying unit (5); the first quantifying unit (5) is used for quantifying a to-be-detected sample and is composed of a first slit (51) as well as a first sample chamber (52) and a second sample chamber (53) which are separated by the first slit (51); thefirst sample chamber communicates with the sampling unit, and the second sample chamber communicates with the waste liquid unit; and a third error reporting unit (14) is arranged between the reactionunit and the waste liquid unit and is provided with a chamber for receiving the to-be-detected sample from the reaction unit. The chip system is composed of three layers including an upper layer, a middle layer and a lower layer; the middle layer is the chip; the upper layer and the lower layer are used for covering and sealing the middle layer; and a sampling hole connected with the sampling unitand a demising hole corresponding to a liquid storage unit are formed in the upper layer. The chip is simple in detection process, capable of quantifying a reaction sample, high in sensitivity and strong in repeatability.

Description

technical field [0001] The invention relates to the technical field of biochemical detection of microfluidic chips, in particular to a biochemical detection microfluidic chip and a biochemical detection microfluidic chip system, and their application in biochemical detection. Background technique [0002] Microfluidic chip technology, also known as microfluidic chip lab or chip lab, refers to a chemical or biological laboratory built on a chip of a few square centimeters. The basic operation units such as sample preparation, reaction, separation, detection, cell culture, sorting, and lysis are integrated on a small chip, and a network of microchannels is formed to run through the entire system with controllable fluids to realize biological, chemical , various functions in fields such as medical diagnosis. [0003] The basic characteristics and biggest advantages of microfluidic chip technology are: a variety of unit structures can be flexibly combined on the tiny chip platf...

AI Technical Summary

Problems solved by technology

After patent research, it was found that there are a small number of related patents using microfluidic technology for biochemical detection at home and abroad in recent years. To drive, the chip needs to be rotated at a high speed, which increases the risk of operation; another example is a multi-index biochemical detection microfluidic chip disclosed in Chinese patent 201210142337, but the chip does not have the reagent storage function, and there is no error reporting mechanism , so it is difficult to guarantee the accuracy of the detection. At the same time, a pneumatic microvalve is used inside the chip to isolate the liquid, so as to realize the quantification of the liquid. However, this method is difficult to control accurately in practical applications, and it is difficult to achieve accurate quantification of the liquid in actual tests

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