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32results about How to "Avoid detection interference" patented technology

Chemiluminescent detection microfluidic chip and chemiluminescent detection microfluidic chip system and application thereof

The invention relates to the technical field of microfluidic chip chemiluminescent immunoassay, in particular to a chemiluminescent detection microfluidic chip and a chemiluminescent detection microfluidic chip system and application thereof. The chemiluminescent detection microfluidic chip comprises a sample feeding unit (1), a liquid storage unit (2), a reaction unit (3) and a waste liquid unit(4), and further comprises a quantifying unit (5) for quantifying a sample to be detected, wherein the quantifying unit (5) comprises a liquid inlet (51) connected with the sample feeding unit, a liquid outlet (52) connected with the waste liquid unit, a quantifying structure (53) for quantifying, and a temporary storage structure (54) for temporarily storing excess liquid, which is connected withthe reaction unit. The chemiluminescent detection microfluidic chip system consists of three layers, namely an upper layer, a middle layer and a lower layer; the middle layer is the chip; the upper layer and the lower layer are used for closing the middle layer; the upper layer is provided with a sample feeding hole connected with the sample feeding unit and a yielding hole corresponding to the liquid storage unit. The chemiluminescent detection microfluidic chip is simple in detection process, can quantify the reaction sample and has high sensitivity and strong repeatability.
Owner:丁锐

Molecular diagnosis micro-fluidic chip, molecular diagnosis micro-fluidic chip system and applications of molecular diagnosis micro-fluidic chip and molecular diagnosis micro-fluidic chip system

The invention relates to the field of micro-fluidic chip molecular diagnosis, and in particular to a molecular diagnosis micro-fluidic chip, a molecular diagnosis micro-fluidic chip system and applications of the molecular diagnosis micro-fluidic chip and the molecular diagnosis micro-fluidic chip system. The chip comprises a sample injection unit (1) which is used for receiving to-be-detected samples, at least one liquid storage unit (2) for storing reaction reagents, a cell lysis unit (3) for conducting cell lysis and a nucleic acid amplification unit (4) for conducting nucleic acid amplification, wherein the cell lysis unit comprises a slit (31) as well as a first compartment (32) and a second compartment (33) which are separated by virtue of the slit; at least one compartment is configured with grinding micro-beads; the first compartment communicates with the sample injection unit; and the second compartment communicates with the at least one liquid storage unit, so as to receive acell lysis solution. The chip system is composed of three layers, namely an upper layer, a middle layer and a lower layer, wherein the chip is arranged on the middle layer; the upper and lower layersare used for covering and sealing the middle layer; the upper layer is provided with a sample injection hole which is connected to the sample injection unit, and the sample injection hole correspondsto an avoiding hole of the liquid storage unit. The chip is simple in detection process, high in sensitivity and strong in repeatability, and reaction samples can be quantified.
Owner:丁锐

Biochemical detection micro-fluidic chip, biochemical detection micro-fluidic chip system and applications thereof

The invention relates to the technical field of biochemical detection of micro-fluidic chips and in particular relates to a biochemical detection micro-fluidic chip, a biochemical detection micro-fluidic chip system and applications thereof. The chip comprises a sampling unit (1), a reaction unit (3) and a waste liquid unit (4) and a first quantifying unit (5); the first quantifying unit (5) is used for quantifying a to-be-detected sample and is composed of a first slit (51) as well as a first sample chamber (52) and a second sample chamber (53) which are separated by the first slit (51); thefirst sample chamber communicates with the sampling unit, and the second sample chamber communicates with the waste liquid unit; and a third error reporting unit (14) is arranged between the reactionunit and the waste liquid unit and is provided with a chamber for receiving the to-be-detected sample from the reaction unit. The chip system is composed of three layers including an upper layer, a middle layer and a lower layer; the middle layer is the chip; the upper layer and the lower layer are used for covering and sealing the middle layer; and a sampling hole connected with the sampling unitand a demising hole corresponding to a liquid storage unit are formed in the upper layer. The chip is simple in detection process, capable of quantifying a reaction sample, high in sensitivity and strong in repeatability.
Owner:丁锐

Lateral flow immune test strip based on ordered micro-nano structure

PendingCN111751525AAvoid cross reactionPrecise electromagnetic field confinement effectMaterial analysisAnalyteNanoparticle
The invention discloses a lateral flow immune test strip based on an ordered micro-nano structure. The lateral flow immune test strip is used for detecting a target in an analyte. According to the test strip, a base is used as a substrate, a sample pad, a conjugate pad, a chromatography pad, an ordered micro-nano structure detection pad and an absorption pad are sequentially distributed on the substrate from left to right, the sample pad is a target loading area, nanoparticles capable of being coupled with a target are combined on the conjugate pad, the ordered micro-nano structure detection pad is arranged on the chromatography pad, and a detection area and a quality control area are distributed on the ordered micro-nano structure detection pad; the sample pad and the conjugate pad are overlapped together, the conjugate pad and the chromatography pad are overlapped together, the ordered micro-nano structure detection pad is fixed on the chromatography pad, and the chromatography pad and the absorption pad are overlapped together; in practical application, when a sample reaches a detection area, multi-target detection can be realized by collecting signals; the test strip has the advantages of short detection time, simplicity and convenience in operation, low cost, simultaneous detection of multiple targets and the like, and is expected to be widely applied to the field of rapiddetection.
Owner:SOUTHEAST UNIV

Automotive door assembly checking fixture and detection method for automotive door assembly

The present invention discloses an automotive door assembly checking fixture which comprises positioning pins (10) which are fixed on the automotive door assembly checking fixture. Each positioning pin (10) comprises a shaft sleeve (11) and a pin (12) which is movably mounted in the shaft sleeve (11). When the automotive door assembly checking fixture detects the edge of the automotive door assembly, the pin (12) is accommodated in the shaft sleeve (11). When the automotive door assembly checking fixture detects an automotive door inner board of the automotive door assembly, the pin (12) extends out of the shaft sleeve (11). Furthermore, the invention also discloses a detection method for detecting the automotive door assembly with the automotive door assembly checking fixture. Because each positioning pin on the automotive door assembly checking fixture according to the invention has a telescopic structure, the detection method for detecting the automotive door assembly through the automotive door assembly checking fixture can prevent detection interference in the detection process for the edge of the automotive door assembly and optimizes detection process of the integral body of the automotive door assembly.
Owner:BEIQI FOTON MOTOR CO LTD

Detection method of electrochemical acute leukemia gene Pax-5a based on enzyme assisted cyclic signal amplification

Provided is a detection method of an electrochemical acute leukemia gene Pax-5a based on enzyme assisted cyclic signal amplification; the efficiency advantage of transcription termination and translation is controlled based on a hairpin DNA probe (HP). The target gene Pax-5a opens HP, and the replication template function of the HP is started. Under the synergistic effect of DNA polymerase and restriction endonuclease, on one hand, auxiliary DNA promotes the target gene Pax-5a to cyclically open the HP; on the other end, a large number of G-quadruplex sequences are produced. The G-quadruplex sequences are captured by a sulfhydryl-modified probe on the surface of a gold electrode due to the complementary action of bases, then a G-quadruplex / hemin complex is formed in the presence of hematinchloride and potassium ions, and hydrogen peroxide can be catalyzed to reduce so as to generate electrochemical signals; the change of the current has linear correlation relationship with the concentration of the target gene in a certain range. Based on the mechanism of enzyme assisted cyclic signal amplification of the hairpin DNA probe and a one-key electrochemical detection platform, high-sensitivity and high-selectivity detection of the target gene can be achieved.
Owner:JIANGXI NORMAL UNIV

Integrated power distribution device installation frame and integrated power distribution device

The invention discloses an integrated power distribution device installation frame which comprises a shell with an installation cavity, a plurality of installation plates are fixed in the installation cavity in the left-right direction, each installation plate is arranged in the front-back direction of the installation cavity, and an installation area for installing a current sensor is formed between every two adjacent installation plates or between the installation plate and a side plate of the shell. At least one installation area is internally provided with a plurality of mounting structures for mounting the current sensors, the plurality of mounting structures are arranged in the front-back direction of the mounting cavity and are different in height, the current sensors can be obliquely mounted on the mounting plate, so that the detection holes of the current sensors are obliquely upward, and the heights of the plurality of current sensors in the installation areas are different. Compared with the prior art, the device is used in multiple power distribution loops and a power distribution device which is provided with multiple current sensors to detect the current of each power distribution loop, so that space is saved, arrangement is compact, the final size of the power distribution device is small, and mutual interference among the multiple power distribution loops is avoided. The invention also discloses a corresponding integrated power distribution device.
Owner:EAST GRP CO LTD

Visual inspection error prevention system and position correction method for articles transported by conveyor belt

The invention discloses a visual inspection error prevention system and a position correction method for articles transported by a conveyor belt, and belong to the field of visual inspection. The system comprise a camera, wherein the camera is erected above a conveying device through a camera bracket; and the system is characterized in that the color of a carrying platform of the conveying deviceis more than one, the various colors are arranged on the carrying platform in a staggered mode, the camera is connected to a controller, and the communication end of the controller is connected to a control system of the conveying device; and an induction device is arranged and used for detecting the advancing position of the conveying device, the signal output end of the induction device is connected to the controller, and the controller controls the camera to shoot after receiving a control signal of the induction device. According to the system, detection interference caused by corresponding consistency of the color of the detected object and the color of the detected object carrying platform is avoided, whether the object is in the center of the carrying platform or not can be detected, and object deviation data can be calculated.
Owner:淄博蓝达智能视觉科技有限公司 +3

Negative plate binder distribution detection method and dyeing device

ActiveCN111141773AAffect structural stabilityGuaranteed dyeingMaterial analysis using wave/particle radiationBrominePhysical chemistry
The invention discloses a negative plate binder distribution detection method and a dyeing device. The negative plate binder distribution detection method comprises the following steps: acquiring a to-be-detected negative plate sample, and dyeing the negative plate sample; in the dyeing process of the sample, placing the negative plate sample in a closed container, and injecting bromine steam intothe closed container containing the negative plate sample; storing the negative plate sample in a closed container filled with the bromine steam for a preset time; and taking out the negative plate sample from the closed container and obtaining the distribution condition of a binder in the negative plate sample. The dyeing treatment is carried out on a negative plate by utilizing the bromine steam, CuBr2 formed by the direct side reaction of bromine steam and a current collector on a section of an electrode coating can be effectively reduced; the interference of the diffusion of the side reaction product CuBr2 in the negative plate active coating on detection of the negative plate cross section binder is avoided, the influence of the side reaction on the structural stability of the plateis avoided, and the accuracy of distribution detection of the negative plate binder is guaranteed.
Owner:HUBEI JINQUAN NEW MATERIALS CO LTD

Detection system and method for broken needle in workpiece

The invention relates to the technical field of needle detectors, in particular to a detection system and method for a broken needle in a workpiece. The detection system comprises at least one detection unit and a control unit, wherein the detection units comprise magnetizing modules for magnetizing a to-be-detected workpiece and magnetism detecting modules for detecting whether the large residual magnetism exists in the magnetized to-be-detected workpiece or not; the control unit is used for collecting the detection data at the magnetism detecting modules and carrying out corresponding processing operation to output a corresponding control instruction. By means of the detection system and method for the broken needle in the workpiece, a broken needle in a weak-magnetism metal workpiece can be well detected.
Owner:NINGBO JIUZONG INTELLIGENT TECH

Intelligent appearance detection tire supporting and expanding rotating mechanism

The invention discloses an intelligent appearance detection tire supporting and expanding rotating mechanism, which is provided with a rack and a supporting and expanding mechanism, wherein the rack is provided with a bottom plate, a front plate and a rear plate are arranged on the bottom plate, a plurality of transverse shafts are arranged between the front plate and the rear plate, a middle plate is arranged between the front plate and the rear plate, four front moving openings are formed in the front plate and include the first front moving opening, the second front moving opening, the third front moving opening and the fourth front moving opening, a front sliding rail is arranged on the inner side of each front moving opening, a front sliding block is arranged on each front sliding rail, four middle moving openings are formed in the middle plate and include the first middle moving opening, the second middle moving opening, the third middle moving opening and the fourth middle moving opening, a middle sliding rail is arranged at each middle moving opening, and a middle sliding block is arranged at each middle sliding rail; and the supporting and expanding mechanism is provided with a first supporting and expanding shaft, a second supporting and expanding shaft, a third supporting and expanding shaft and a fourth supporting and expanding shaft. The mechanism is small in occupied area and convenient to detect, the manual carrying time and detection time are saved, and the automation efficiency is improved.
Owner:HANGZHOU XIANSHUN AUTOMATION TECH

Road disaster remote sensing intelligent detection method based on deep learning

PendingCN112990100ANarrow detection rangeSolve the problem that the road disaster target itself cannot be directly detectedCharacter and pattern recognitionNeural architecturesImage resolutionEngineering
The invention discloses a deep learning-based road disaster remote sensing intelligent detection method. The method comprises the following steps of A, image preprocessing: performing radiometric calibration, atmospheric correction, geometric correction, ortho-rectification and image registration on a remote sensing image; B, establishing a road buffer area: according to the road vector data and the image resolution, establishing an area 3-4 times based on the road width as the road buffer area; C, image masking: performing masking processing on the remote sensing image according to the established road buffer area; D, image block cutting: cutting the masked remote sensing image into a plurality of image blocks; E, image block prediction: inputting the cut image blocks, namely effective image blocks, into the trained YOLOV3 network, and predicting the probability that the image blocks belong to different damage categories of the road; and F, merging the image blocks: merging the predicted image blocks to generate an image result image with the original size. The method is convenient and easy to operate, and an accurate road disaster detection distribution result is obtained.
Owner:AEROSPACE INFORMATION RES INST CAS

Battery charging control method

The invention discloses a battery charging control method, which comprises the following steps of: charging a battery under the control of a single chip microcomputer serving as a controller, disconnecting a charging circuit after the battery is charged for a preset period time, returning the battery voltage to the actual voltage, and detecting the voltage after the battery voltage is reduced to be stable, so that the detection interference caused by the charging voltage can be avoided; compared with an existing method for charging the battery through charging equipment, the method has the advantages that the charging effect is better, the phenomenon that the battery is overcharged is avoided, when the battery is fully charged, the voltage of the battery is closer to the full-charge voltage, namely the rated voltage, of the rechargeable battery, the performance of the battery is improved, and the service life of the battery is prolonged; in addition, according to the scheme, the single-chip microcomputer is selected as a controller to control charging, and the cost is reduced.
Owner:深圳宇凡微电子有限公司

Screening equipment provided with flatness detection mechanism and used for electronic product production

The electronic product production screening equipment with the flatness detection mechanism comprises a conveying unit and a detection unit, the detection unit is arranged on the middle section of the conveying unit, a feeding unit is arranged on one side of one end of the conveying unit, and a control unit is arranged on the other side of the feeding unit. A discharging unit is arranged on the other side of the conveying unit, the conveying unit comprises a detection table, and an electric sliding rail is arranged at the top end of the detection table. According to the screening equipment provided with the flatness detection mechanism and used for electronic product production, the moving base, the clamping air cylinder, the clamping plates and the limiting plates are arranged, when a product is pushed to the moving base, the clamping air cylinder pushes out the clamping plates, the clamping plates and the limiting plates clamp the product from the two sides, and the product moves to the detection unit along with the moving base to be subjected to surface flatness detection; according to the mechanism, through automatic clamping and whole-course conveying, the detection speed is greatly increased, and the problem of low moving stability is solved.
Owner:刘君

Rapid positioning device for shearing of plate shearing machine

The invention discloses a rapid positioning device for shearing of a plate shearing machine. The rapid positioning device comprises a machining bottom plate, a protective cover is installed at the top of the machining bottom plate, a mark positioning structure is installed on the inner top wall of the protective cover, a positioning detection plate is installed on the inner top wall of the protective cover, and a photosensitive induction area is installed at the bottom of the positioning detection plate. A supporting rod is installed on one side of the top of the positioning detection plate, an angular velocity sensor is installed on the surface of the supporting rod, a display screen is installed on the outer wall of one side of the positioning detection plate and electrically connected with the angular velocity sensor, a suction cup is installed on the top of the supporting rod, L-shaped magnetic attraction supports are installed on the front face and the surface of the supporting rod, and a rotating support is mounted on the other side of the top of the positioning detection plate. Through the arrangement of the positioning detection plate, adjustment type positioning detection is achieved, meanwhile, plate pushing can be stopped for shearing treatment when the cutting requirement is not met, and the cutting quality is guaranteed.
Owner:南通东海机床制造集团有限公司

A control method and control device for a pwm rectifier

A control method and a control device for a PWM rectifier, the fault of the input source of the PWM rectifier can be accurately and quickly detected, thereby ensuring the reliability of fault detection. The control method of the PWM rectifier includes: detecting the DC bus voltage of the PWM rectifier; when the DC bus voltage of the PWM rectifier is lower than a set value, controlling the PWM rectifier to be in a rectification mode, and energy flows from an input source of the PWM rectifier to a load.
Owner:HUAWEI DIGITAL POWER TECH CO LTD

Gas detector protective cover capable of preventing electromagnetic interference

The invention discloses a gas detector protective cover capable of preventing electromagnetic interference. The detector comprises a transparent cover, a sensor adapter, a cable lead-in assembly, a sealing plug, a rear cover, a detector body, a hinge, a front cover, hasp locks, hasps, an insulating plate and a glass screen; the transparent cover is fixed on one side surface of the detector body; the two sides of the detector body are symmetrically and fixedly provided with the cable leading-in assembly and the sealing plug. The sensor adapter is fixed at the bottom of the detector body; the detector body is sleeved with the rear cover. One side of the rear cover is rotationally connected with the front cover through the hinge; the hasp locks are symmetrically fixed on one side, far away from the hinge, of the rear cover; and the hasps are symmetrically fixed at one side, far from the hinge, of the front cover. According to the device, a magnetic field can be effectively isolated, the detection interference of the magnetic field on the gas detector can be prevented, the gas detector can be well protected, the damage to the gas detector caused by outside collision can be reduced, andthe service life of the gas detector can be prolonged.
Owner:长兴辰源电子科技股份有限公司

Method and system for detecting passage of objects

The invention relates to a method and system for detecting the passage of an object. The method includes the following steps: (a) providing an object passage area; (b) setting a wireless identification reader on one side of the object passage area. The wireless identification reader has a first polarized antenna. The first polarized antenna can transmit a first polarized wave to a metal part of a passing object. The metal part can reflect the first polarized wave and convert the first polarized wave. Converting the polarized wave into a second polarized wave with the opposite polarization direction; and (c) setting a wireless identification tag in the reflection direction of the first polarized wave, the wireless identification tag having a second polarized antenna, the The polarization direction of the second polarized antenna is opposite to the polarization direction of the first polarized antenna, so that the second polarized antenna can receive the second polarized wave and activate the wireless identification tag, thereby determining Object passes.
Owner:CHINA STEEL

Method for measuring krypton, xenon and methane in air separation oxygen and equipment for measuring krypton, xenon and methane in air separation oxygen

The invention relates to the technical field of gas measurement, and in particular relates to a method for measuring krypton, xenon and methane in air separation oxygen and equipment for measuring krypton, xenon and methane in air separation oxygen. According to the method, methane is removed, so that interference of methane on krypton detection is avoided, methane is converted into carbon dioxide, the carbon dioxide is tested, the purpose of accurately determining the content of methane is further achieved, oxygen is removed, krypton peaks are prevented from being covered by oxygen peaks, damage of oxygen to a detector is also prevented, and the detection precision of krypton, xenon and methane is improved.
Owner:中昊光明化工研究设计院有限公司

Intelligent formaldehyde detector

The invention provides an intelligent formaldehyde detector, capable of automatically performing intelligent formaldehyde concentration classification detection according to preliminary formaldehyde concentration data detected by a formaldehyde detector. When a sensor preliminarily detects a fact that formaldehyde concentration is relatively low, specific formaldehyde concentration is identified by using a reagent method, a user is reminded of residual reserve level of a formaldehyde absorption reagent and the user is reminded to add a formaldehyde absorption reagent box; and when the sensor preliminarily detects a fact that the formaldehyde concentration is relatively high and toxicity can be produced to a human body, the formaldehyde concentration is further detected by using a gas chromatography method, the user is prompted to open an air purification channel as soon as possible, after a period of time, the formaldehyde concentration is detected by continuously using the gas chromatography method, and if the formaldehyde concentration after a period of time is continuously increased and reaches a concentration harmful to the human body, the user is prompted to be evacuated as soon as possible.
Owner:汪梅荣

Method for measuring content of aluminum in silicon-calcium alloy

The invention provides a method for measuring the content of aluminum in a silicon-calcium alloy, the method comprises the following steps: only dropwise adding hydrofluoric acid into a sample to be measured for reaction, after the reaction is stopped, heating and evaporating on an electric hot plate, firing at high temperature, then directly adding a flux formed by grinding and mixing anhydrous potassium carbonate and boric acid, melting in a high-temperature furnace, leaching with hydrochloric acid, and fixing the volume, and finally, measuring the content of aluminum by adopting instrument analysis methods such as inductively coupled plasma atomic emission spectrometry and the like. Only hydrofluoric acid is added in the sample pretreatment process so that the use of a large amount of acid is avoided; by utilizing the characteristic that hydrofluoric acid can react with silicon under high-temperature heating to generate a volatile substance silicon tetrafluoride (SiF4), a large amount of interference element silicon in the sample is removed; residues are burned at high temperature, and part of detection interference impurities are removed; residues are directly dissolved with hydrochloric acid after being subjected to high-temperature alkali fusion, and are directly detected after constant volume so that the tedious operation caused by combining a main solution obtained after acid dissolution with a diluent obtained after alkali fusion in the past is avoided.
Owner:PANZHIHUA IRON & STEEL RES INST OF PANGANG GROUP +1

Door assembly inspection tool

The invention discloses a vehicle door assembly inspection tool. The vehicle door assembly inspection tool includes a positioning pin (10) fixed on the vehicle door assembly inspection tool. The positioning pin (10) includes a shaft sleeve (11) and a movable mounting The pin shaft (12) in the shaft sleeve (11), when the door assembly inspection tool detects the edge of the door assembly, the pin shaft (12) is stored in the shaft sleeve (11); When the vehicle door assembly inspection tool detects the vehicle door inner panel of the vehicle door assembly, the pin shaft (12) protrudes from the shaft sleeve (11). In addition, the invention also discloses a detection method for detecting the vehicle door assembly by using the above-mentioned vehicle door assembly inspection tool. Since the locating pin on the vehicle door assembly inspection tool of the present invention is a retractable structure, the detection process at the edge of the vehicle door assembly can be avoided by using the vehicle door assembly inspection tool to detect the vehicle door assembly. In the detection interference, the overall detection process of the door assembly is optimized.
Owner:BEIQI FOTON MOTOR CO LTD

PH value detection method and device based on image processing

The invention relates to a pH value detection method and a pH value detection device based on image processing, wherein a pulse signal sent by a single-chip is sent to a stepper motor, a connection rod is driven to rotate by the stepper motor, fluid to be detected is injected to pH test paper through an injection orifice of the fluid to be detected when an external end of the pH test paper falls to the center of the injection orifice of the fluid to be detected, and the pH test paper is shot by a CCD (charge coupled device) sensor under the light condition of an illuminating lamp when the pH test paper continuously falls to the center of a through groove in an image acquisition device box, so that the image acquisition of the color change signals is finished; and the acquired images are input into the single-chip for preprocessing and then are converted into an HIS color space, three characteristic value data of the color change blocks including H, I and S in the space are extracted, three characteristic values are input into a relation model both of a pre-built color block and the pH value, and the pH value is calculated, so that the online automatic detection of the pH value is realized and the influence of the human factors in the artificial reading process is avoided.
Owner:JIANGSU UNIV

Plasma mass spectrum ion focusing deflection sample introduction interface and working mode

The invention discloses a plasma mass spectrometry ion focusing deflection sample introduction interface and a working mode, and relates to an ion optical device. The interface comprises a plasma source for forming plasma through a gas ionization mode; a plurality of focusing deflection plates, wherein each focusing deflection plate is provided with a circular central channel, deflection electrode pairs are distributed on the edge of each circular central channel, and the plurality of focusing deflection plates are distributed between the plasma source and the mass spectrum sample introduction interface at a certain angle relative to the motion trail of the plasma. According to the invention, effective separation of photons and neutral particles and ion focusing are simultaneously realized in the ion transmission process.
Owner:INST OF ANALYSIS GUANGDONG ACAD OF SCI (CHINA NAT ANALYTICAL

Method for determining desoxyquinocetone and 3-methylquinoxaline-2-carboxylic acid in seawater

The invention provides a method for determining desoxyquinocetone and 3-methylquinoxaline-2-carboxylic acid in seawater, and belongs to the technical field of detection of pollutants in seawater. According to the method, the MAX column is adopted to enrich and purify the desoxyquinocetone and the 3-methylquinoxaline-2-carboxylic acid in the seawater, so that the determination of the low-concentration desoxyquinocetone and the 3-methylquinoxaline-2-carboxylic acid in the seawater can be realized; and according to the method, the adsorption column is eluted with water to remove most of water-soluble impurities, and then the adsorption column eluted with water is eluted with the ethyl acetate solution of formic acid, so that the content of other substances in the to-be-detected liquid can be reduced, interference on detection of desoxyquinocetone and 3-methylquinoxaline-2-carboxylic acid is avoided, and the detection sensitivity is improved.
Owner:山东省海洋资源与环境研究院

Expression, purification and application of E.coli O157:H7 flagellin H7 antigen segment

The invention discloses expression, purification and application of (i) E.coli ( / i) O157:H7 flagellin H7 antigen segment, relating to the field of genetic engineering technology, vaccine and diagnostic reagent. According to computer analysis, strong antigen epitope in (i) E.coli ( / i) O157:H7Flic protein is screened out, totally 131 amino acid, from 215th amino acid to 345th amino acid, codon preferred by prokaryote is selected for chemical synthesis of a brand-new gene sequence of the antigen epitope, by genetic engineering technology, the gene segment is expressed, and the strong antigen epitope segment of the (i) E.coli ( / i) O157:H7Flic protein is prepared. The expressed protein can be used for detection of (i) E.coli ( / i) O157:H7 infected antibody and preparation of monoclonal antibody and polyclonal antibody.
Owner:中国人民解放军东部战区疾病预防控制中心
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