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Anti-oxidation peptide composition and preparation method and application thereof

A technology of antioxidant peptides and compositions, applied in applications, cosmetic preparations, cosmetics, etc., can solve the problems of oligopeptides with many impurities, difficult purification, complicated methods, etc., and achieve high extraction rate, good purification effect, and preparation methods simple effect

Pending Publication Date: 2018-06-22
GUANGZHOU KENENG COSMETICS RES CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is complex, requires the use of multiple enzymes, and the cost is high, and the prepared oligopeptides contain many impurities and are not easy to purify

Method used

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  • Anti-oxidation peptide composition and preparation method and application thereof
  • Anti-oxidation peptide composition and preparation method and application thereof
  • Anti-oxidation peptide composition and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0044] The present invention also provides a preparation method of the antioxidant peptide composition according to the present invention, including the step of using microorganisms for fermentation and culture.

[0045] In the present invention, the microorganisms include one or a combination of two or more of Bacillus subtilis, Bacillus natto and Aspergillus oryzae. By using microorganisms, an antioxidant peptide composition with high polypeptide content and excellent antioxidant performance can be prepared.

[0046] Specifically, Bacillus subtilis (Bacillus subtilis) is an aerobic spore-forming Gram-positive bacterium, which has rich and diverse physiological characteristics, is widely distributed, and is very easy to isolate and culture. The bacterium exists widely in nature, is non-toxic and harmless to humans and animals, does not pollute the environment, can produce a variety of antibiotics and enzymes, has broad-spectrum antibacterial activity and strong resistance to ...

Embodiment 1-24

[0081] Antioxidant peptide compositions were prepared according to different preparation conditions shown in Tables 1-4. Wherein, the preparation method of the antioxidant peptide composition comprises the following steps:

[0082] Pretreatment step: Add Ulva dry powder to distilled water for ultrasonic dispersion and dissolution; wherein, the volume of distilled water is 10-50mL / g based on the mass of Ulva dry powder, the ultrasonic power is 250-350W, the ultrasonic frequency is 25-30kHz, and the ultrasonic temperature is 25°C , the ultrasonic time is 10-20min; then filter to remove impurities; then add NaOH solution with a mass concentration of 1-5% to adjust the pH value to 6.5-7.5, sterilize in an autoclave at 121°C for 15min, and cool to 25 ℃ to prepare the Ulva algae solution.

[0083] Preparation of the culture solution: the culture solution is mixed with Bacillus subtilis, Bacillus natto and Aspergillus oryzae in a mass ratio of 10:1-10:1-10. Wherein, the culture med...

Embodiment 25-30

[0095] According to the method of Examples 1-24, the antioxidant peptide composition was prepared according to different preparation conditions shown in Table 5.

[0096] table 5

[0097]

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Abstract

The invention provides an anti-oxidation peptide composition and a preparation method and application thereof. The anti-oxidation peptide composition is derived from fermented products of ulva algae.The anti-oxidation peptide composition is prepared from polypeptides, free amino acids and polysaccharides. Based on the total mass of the anti-oxidation peptide composition, the content of the polypeptides is 6-85%, preferably 18-85%, and more preferably 30-85%; the content of the free amino acids is 1-20%, preferably 4-18%, and more preferably 8-16%; the content of the polysaccharides is 1-15%,preferably 3-13%, and more preferably 4-12%; and the molecular weight range of the polypeptides is 238Da-160kDa, preferably 238Da-100kDa, and more preferably 238Da-50kDa. The anti-oxidation peptide composition provided by the invention can be applied to many fields of food, health-care products, daily chemical products and the like; especially, when the anti-oxidation peptide composition is applied to cosmetics, the anti-oxidation peptide composition can achieve the effects of scavenging free radicals and improving the anti-oxidation and anti-aging capabilities of skin; and the anti-oxidationpeptide composition is low in cost.

Description

technical field [0001] The invention relates to an antioxidant peptide composition and its preparation method and application, which can be used in the fields of health care products, food, nutritional supplements, animal feed, cosmetics, daily chemical products and the like. Background technique [0002] Antioxidants are widely used in food, health products, daily chemical products and other fields. On the one hand, antioxidants can prevent the above products from discoloration or deterioration due to oxidation; on the other hand, some oxidants even play active functions in related products, such as anti-oxidation and scavenging free radicals in the body. At present, chemically synthesized antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-p-cresol (BHT) are widely used in the cosmetic industry, but these synthetic antioxidants are due to potential dangers to human health. , its application is increasingly limited. [0003] At present, the research on a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472A23L33/105A61K8/64A61K8/9706A61K8/73A61K8/44A61Q19/08
CPCA23L3/3472A61K8/44A61K8/64A61K8/73A61K2800/10A61K2800/522A61K2800/85A61Q19/08A23L33/105
Inventor 李传茂刘德海刘培崔龙生张伟杰张楚标
Owner GUANGZHOU KENENG COSMETICS RES CO LTD
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