A gold nanoprobe-based method for the identification of individual Chlamydia pneumoniae

A Chlamydia pneumoniae and gold probe technology, applied in the field of pathogen detection, can solve the problems of low sensitivity, false positive, aerosol pollution, etc., and achieve high sensitivity

Active Publication Date: 2021-03-09
YANGZHOU UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method has a big disadvantage: low sensitivity, low signal-to-noise ratio, inaccurate
However, the LAMP method has a serious disadvantage: once the lid is opened, it is easy to form aerosol pollution, and the problem of false positives is more serious.
Therefore, it is necessary to use a real-time turbidity analyzer. In addition, the LAMP method has relatively high requirements for primer design, and some disease genes are not suitable for the LAMP method.

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  • A gold nanoprobe-based method for the identification of individual Chlamydia pneumoniae
  • A gold nanoprobe-based method for the identification of individual Chlamydia pneumoniae
  • A gold nanoprobe-based method for the identification of individual Chlamydia pneumoniae

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Embodiment Construction

[0040] The present invention will be further explained below in conjunction with the accompanying drawings, but the present invention is not limited by the embodiments.

[0041] The detection of Chlamydia pneumoniae includes the following steps:

[0042] 1. Materials

[0043] Gold nanoparticles coated with protein A were purchased from Creative Diagnostics, USA; rabbit anti-Chlamydia pneumoniae polyclonal antibody was purchased from Fitzgerald, USA; Chlamydia pneumoniae was a laboratory-preserved strain with serotype CWL029; the centrifuge was purchased from Eppendorf, Germany ; Hula Mixer was purchased from Norway life technology company; ultrasonic instrument was purchased from Kunshan Ultrasonic Instrument Co., Ltd., China.

[0044] 2. Experimental method

[0045] The specific process of the experiment is attached figure 1 As shown, the specific steps are as follows:

[0046] 1. Cleaning of Gold Nanoparticles

[0047] Add 50 μL of commercially available gold nanoparticl...

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Abstract

The invention discloses a method for identifying a single Chlamydia pneumoniae based on a gold nanoprobe. The method is to combine gold nanoparticles coated with commercialized protein A with a commercialized Chlamydia pneumoniae antibody to construct a gold probe, and combine it with the pneumoniae in the detection sample. The chlamydia were incubated and observed directly with the naked eye using a dark-field microscope. The invention constructs a gold probe combined with chlamydia pneumoniae, and establishes a rapid detection system for chlamydia pneumoniae. The gold probe-based dark-field microscope naked eye observation method is simple, fast, and does not require special equipment. It can be used to identify whether a sample contains Chlamydia pneumoniae in the field.

Description

technical field [0001] The invention belongs to the field of pathogen detection, and in particular relates to a method for identifying a single chlamydia pneumoniae based on a gold nanometer probe. Background technique [0002] Chlamydia pneumoniae (Cpn for short) is a common zoonotic pathogen. It is a prokaryotic microorganism that parasitizes obligately within cells. It is a new species of Chlamydia established in 1989. Chlamydia pneumoniae is transmitted through the respiratory tract. At least 50% of people in the world have been infected with Chlamydia pneumoniae. It is a common pathogen of respiratory tract infections, such as pneumonia, bronchitis, and sinusitis. In recent years, it has been found that Chlamydia pneumoniae may be one of the causes of atherosclerosis. one. Because Chlamydia pneumoniae has no specific detection symptoms in clinical symptoms, further laboratory tests are needed to confirm the cause, and it is sensitive to the external environment. There...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N21/84
CPCG01N21/84G01N33/56927G01N2800/12
Inventor 周昕邸涛
Owner YANGZHOU UNIV
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