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Compound enzyme and application thereof, additive and application thereof, and method for removing zearalenone or alpha-zearalenol

A technology of zearalenol and zearalenone, which is applied in the field of compound enzymes, can solve the problems that the degradation rate of ZEN needs to be further improved, the pollution of grain, oil or feed products, and the impact on the palatability of grain, oil or feed, etc. Application prospect and effect of improving removal rate

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +4
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  • Claims
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AI Technical Summary

Problems solved by technology

However, when the above-mentioned bacteria or fungi are applied to ZEN degradation in grain, oil or feed, it is likely to have a certain impact on the palatability of grain, oil or feed, and the degradation rate of ZEN needs to be further improved.
[0006] However, so far, there have been few reports on the simultaneous degradation of ZEN and its main metabolite α-ZOL by biological methods. Since α-ZOL is likely to be metabolized in plants and contaminate grain, oil or feed products, it is very important to It is necessary to find a biological method that can simultaneously degrade ZEN and its main metabolite α-ZOL in grain, oil or feed

Method used

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  • Compound enzyme and application thereof, additive and application thereof, and method for removing zearalenone or alpha-zearalenol

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preparation example Construction

[0025] The present invention also provides a preparation method of the above compound enzyme, which comprises: mixing lactonase and laccase, and the weight ratio of the lactonase to the laccase is 1:1-10.

[0026] In order to further improve the removal effect of the complex enzyme on ZEN or α-ZOL, preferably, the weight ratio of the lactonase to the laccase is 1:2-8, more preferably 1:4-6 .

[0027] Preferably, the preparation method of the above-mentioned composite enzyme comprises: mixing lactonase, laccase and copper agent, and relative to the sum of 100 parts by weight of the lactonase and the laccase, the copper agent The amount used is 0.001-0.05 parts by weight, preferably 0.01-0.05 parts by weight.

[0028] In the present invention, the copper agent can be any copper agent (source of copper ions, that is, a substance that can provide copper ions) that can be added to grain, oil and / or feed conventionally used in the art; preferably, the The copper agent is sodium co...

preparation example 1

[0048] This preparation example is used to illustrate the preparation method of lactonase.

[0049] According to the sequence disclosed in CN105755023A (SEQ ID NO.1), Bao Biological Engineering (Dalian) Co., Ltd. was entrusted to artificially synthesize the corresponding gene fragment, and the gene fragment was cloned into the vector PET30a plasmid (with His tag, purchased at U.S. Invitrogen Company) obtains the recombinant vector, transforms the recombinant vector into BL21 (DE3) Escherichia coli strain (purchased from Beijing Huayueyang Biotechnology Co., Ltd., article number is NRR01210, the same below), and makes BL21 (DE3) Escherichia coli strain UDP-glucosyltransferase was expressed to obtain a recombinant strain. Pick a single colony and inoculate it into LB liquid medium (LB medium: 5 grams of beef extract, 10 grams of peptone, 5 grams of sodium chloride, make up water to 1000 ml, sterilize at 115°C for 20 minutes, pH 7.0), at 37 Cultivate to OD 600 If the value is b...

preparation example 2

[0052] This preparation example is used to illustrate the preparation method of lactonase.

[0053] Carry out according to the method of preparation example 1, difference is, according to GenBank No. is the sequence shown in AB076037.1 artificial chemical synthesis corresponding gene fragment, to replace the gene fragment used in embodiment 1, to make lactonase dry powder A2 .

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Abstract

The invention relates to the field of microorganisms, and discloses a compound enzyme and an application thereof, an additive and an application thereof, and a method for removing zearalenone or alpha-zearalenol, and particularly provides the compound enzyme. The compound enzyme contains lactonase and laccase in the weight ratio of the lactonase to the laccase being 1 to (1-10). The lactonase andthe laccase are combined for use, so that the obtained compound enzyme can remove ZEN and main metabolism products namely alpha-ZOL efficiently and safely, particularly when the compound enzymes is used to grain and oil or feeds, the ZEN and alpha-ZOL can be removed efficiently and safely, and the palatability of the grain and oil or feeds cannot be influenced; and the compound enzyme has favorable industrial application prospect. In addition, in one preferred embodiment, the compound enzyme also contains a copper agent, such as sodium copper chlorophyllin salts and / or potassium copper chlorophyll salts, and can further increase the removing rate of the ZEN and main metabolism product namely alpha-ZOL.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to a composite enzyme, an additive containing the composite enzyme, and the composite enzyme and / or the additive are used in removing zearalenone and / or α-zearalenol application, and a method for removing zearalenone and / or α-zearalenol. Background technique [0002] Zearalenone (Zearalenone, ZEN) is a mycotoxin produced by Fusarium fungus and released into the soil environment. It is the most widely polluted Fusarium toxin in the world. In 1999, D. Mello et al. found that ZEN can reduce the embryo survival rate and the birth rate of newborn fetuses in pregnant animals. The impact of ZEN on the human body is mainly to induce tumors, induce DNA shrinkage, and cause chromosomal abnormalities. In addition, ZEN can bind to 17β-estradiol receptors, leading to fat oxidation, cell apoptosis, inhibition of protein and DNA synthesis, and inhibition of biosynthesis of other macromolecu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L5/20
Inventor 苏会波林海龙李凡陈博周勇郭翠李久仁樊维荣佟易李义
Owner COFCO NUTRITION & HEALTH RES INST
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