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Primer Combination and Identification Method for Identifying Parentage Relationship of Wagyu Three-way Hybrid Cattle

A technology of ternary hybridization and parental relationship, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/testing, etc., can solve the problems of high price and low applicability, and achieve the effect of improving test efficiency

Active Publication Date: 2021-08-03
INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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Problems solved by technology

[0006] The present invention aims at improving the existing commercial kits, which are expensive and have low applicability to Wagyu three-way hybrid cattle populations, and specifically selects high-throughput, accurate and efficient nine bovine microsatellite markers for amplification and typing, these markers are highly polymorphic, unlinked between markers and have appropriate fragment size intervals, which are easy to detect at the same time

Method used

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  • Primer Combination and Identification Method for Identifying Parentage Relationship of Wagyu Three-way Hybrid Cattle
  • Primer Combination and Identification Method for Identifying Parentage Relationship of Wagyu Three-way Hybrid Cattle

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Embodiment 1

[0043] The screening of embodiment 1 microsatellite marker primer

[0044] The inventor refers to the microsatellite marker combination ( http: / / dad.fao.org / ), obtained the primer set of the present invention through screening.

Embodiment 2

[0045] Example 2 Identifying the parental relationship of Wagyu three-way hybrid cattle

[0046] 1. Bovine Blood Collection

[0047]The samples of the present invention come from Wagyu and Wagyu three-way hybrid cattle, a total of 17 samples, including 2 parts of frozen semen of Wagyu male parent, N1, N2; A4 is the descendant of N2 bull, B1-B3 is the descendant of N1 bull, and C1-C4 is the descendant of other families. All individuals lacked maternal information. Take oxtail vein blood collection method: bind the ox, sterilize the blood collection site in the depression at the midpoint of the junction of the fourth to fifth tail vertebrae, about 10 cm away from the root of the oxtail, and then stab the ventral center of the oxtail vertically with a disposable blood sampling device Blood was collected at a depth of about 0.5 cm from the line position, and after the blood collection was completed, an alcohol cotton ball was used to press the pinhole to stop bleeding. The coll...

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Abstract

The invention discloses a combination of primers and an identification method for identifying the parentage relationship of Wagyu three-way hybrid cattle. The present invention screens out 9 fluorescently labeled microsatellite primer combinations with rich polymorphisms, performs PCR amplification with beef cattle genomic DNA to be tested as a template, performs capillary electrophoresis on the amplified fragments to detect fluorescent signals, and judges each individual's identity according to the detection results. Genotype: According to the genotype, calculate the PI value of single-parent parentage index and the relative probability value of parent-child W value to judge the parentage relationship. The identification method of the invention is simple, fast, low in cost, efficient, economical, easy to implement and the like, can improve the accuracy of beef cattle selection and mating, speed up the breeding process, and has good application prospects and economic benefits.

Description

technical field [0001] The invention belongs to the field of animal breeding, and in particular relates to a method and a special primer combination for carrying out paternity identification on the offspring of Wagyu three-way hybrid cattle by using fluorescent microsatellite labeling technology. Background technique [0002] my country is a big beef consumer, but the output and quality of domestic beef cannot meet the needs of Chinese consumers, and it is unrealistic to completely import high-quality beef cattle from abroad. It is an economical and practical way to breed crossbred beef cattle with foreign excellent beef cattle as male parents and Chinese local excellent yellow cattle as female parents. [0003] Cheng Haijian et al. In the article "Preliminary report on the fattening experiment of three-way hybrid steers of Japanese black Wagyu" (Chinese Cattle Industry Science 2016, 42(3): 1-3; 21), it is recorded: "Japanese black Wagyu is a unique beef cattle in Japan. Va...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 张清峰宋恩亮成海建姜富贵秦巧梅刘晓牧万发春
Owner INST OF ANIMAL SCI & VETERINARY MEDICINE SHANDONG ACADEMY OF AGRI SCI
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