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Culture medium for rooting of bletilla striata strong seedlings and application of culture medium

A technology for rooting strong seedlings and culture medium, which is applied in the field of culture medium for strong seedlings and rooting of Bletilla striata, can solve the problems of low reproduction coefficient, reduction of wild natural resources and the like, achieves improvement of survival rate, is conducive to the survival of hardened seedlings, and has a good rooting rate. Effect

Inactive Publication Date: 2018-07-10
黔东南民族职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wild bletilla striata has been over-harvested due to demand, resulting in a sharp decrease in its wild natural resources and is on the verge of extinction. It is listed as one of the key protected wild medicinal plants by the state. The traditional artificial cultivation method is usually propagated by division, and the reproduction coefficient is low. (Huang Taikang and Kong Lingyi, 2002)
In order to protect the wild resources of Bletilla striata, artificial breeding and cultivation of Bletilla striata has become the research direction of research and development personnel, such as the aseptic germination and tissue culture of Bletilla striata, but it is divided into three stages of cultivation using three media, and the germination and rooting efficiency are still to be determined. to further improve

Method used

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  • Culture medium for rooting of bletilla striata strong seedlings and application of culture medium
  • Culture medium for rooting of bletilla striata strong seedlings and application of culture medium
  • Culture medium for rooting of bletilla striata strong seedlings and application of culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0021] The method of rapid propagation of bletilla striata:

[0022] (1) Aseptic germination of seeds: select uncracked and mature Bletilla striata capsules, wash the surface of the capsules with detergent water, rinse with tap water, soak in 75% alcohol for 30S on the ultra-clean workbench, and rinse with sterile water for 3-4 Sterilize with 0.1% mercuric chloride for 8-10 minutes, rinse with sterile water for 7-8 times, dry the surface moisture of the capsule with sterile paper, peel off the middle of the capsule, take out the seeds, and quickly inoculate them on the germination medium , inoculate 10 bottles for each treatment, inoculate 4 clumps in each bottle, observe and record regularly, and analyze the statistical results after 20 days; culture conditions: culture temperature 25±1℃, light intensity 1500Lx, light time 10h·d-1; germination culture The base is: MS+6-BA1.5mg / L+NAA0.8mg / L+AC0.5g / L+proline 0.3g / L+sucrose 30g / L+agar 4g / L, pH5.8. Such as figure 1 As shown, th...

Embodiment 2-11

[0026] The step is basically the same as that of Example 1, and in the embodiment 2-11 step (2), the rooting culture stage of strong seedlings is carried out with the medium combination listed in Table 1 for test statistics, and finally with rooting rate, plant height, pseudobulb diameter, single fibrous root The advantages and disadvantages of the culture medium were investigated by counting, the unmarked components were the same as in Example 1, and the rest of the steps were not repeated.

[0027] Table 1 Selection list of rooting medium for strong seedlings of Bletilla striata

[0028]

[0029]

[0030] The data of rooting rate, seedling height, diameter of pseudobulb, and number of fibrous roots in Table 1 are the average value of the data of 10 bottles, and the robustness ranges from poor to easy to use "+", "++", "+++", "+ +++" level representation.

[0031] It can be seen from Table 1 that the rooting rate of the culture medium in Example 7 reaches 97.9%, and th...

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Abstract

The invention relates to a culture medium for rooting of bletilla striata strong seedlings. The culture medium is prepared by adding the following components onto a base culture medium: naphthylaceticacid (NAA) of 1.5 mg / L, indolebutyric acid (IBA) of 0-0.5 mg / L, banana slurry of 60-100g / L, activated carbon (AC) of 0.3-0.5g / L, proline of 0.3-0.5g / L, sucrose of 30-40 g / L and agar of 4-7g / L, and the pH value of the medium is 5.6-5.8. With the culture medium provided by the invention, cultured test-tube plantlet plants have good rooting rate, high plant height, large diameter of pseudobulb, andabundant fibrous roots of a single root. The best rooting rate can reach 97.9%, the growth of induced roots is good, the strong degree of the plants is good, a solid foundation is laid for the transplantation in a next step and the improvement of the survival rate, and the diameter of the pseudobulb of the test-tube plantlet plants cultured by utilizing the culture medium provided by the inventioncan reach 0.7cm or above.

Description

technical field [0001] The invention belongs to the technical field of medicinal plant cultivation, and relates to a rooting medium for bletilla striata strong seedlings and an application thereof. Background technique [0002] Bletilla striata (Bletilla striata) is a perennial herbaceous plant belonging to the genus Bletilla in Orchidaceae. Bletilla striata not only has high ornamental value, but also is one of the commonly used Chinese medicinal materials in my country (Flora of China, 1999). Bletilla striata pseudobulb is a traditional hemostatic drug. Its active ingredients include Bletilla striata jelly and Bletilla striata polysaccharides, which have the effects of astringent, hemostasis, swelling and muscle growth (Chinese Pharmacopoeia, 2015). ; Because of its special viscosity characteristics, it can also be used as a thickener, suspending agent, humectant and co-emulsifier in cosmetics, with good effects (Liu Guangbin et al., 2005; Ma Shihong et al., 2009), and has...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄刚付燕周黎万新屏郑晓峰何宪江李杰
Owner 黔东南民族职业技术学院
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