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A kind of method of rapidly propagating bletilla striata

A bletilla striata, rapid technology, applied in the field of rapid propagation of bletilla striata, can solve the problems of low reproduction coefficient and reduction of wild natural resources, and achieve the effects of reducing pollution rate, fewer steps, and simplifying production procedures

Inactive Publication Date: 2019-11-05
黔东南民族职业技术学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Wild bletilla striata has been over-harvested due to demand, resulting in a sharp decrease in its wild natural resources and is on the verge of extinction. It is listed as one of the key protected wild medicinal plants by the state. The traditional artificial cultivation method is usually propagated by division, and the reproduction coefficient is low. (Huang Taikang and Kong Lingyi, 2002)
In order to protect the wild resources of Bletilla striata, artificial breeding and cultivation of Bletilla striata has become the research direction of research and development personnel, such as the aseptic germination and tissue culture of Bletilla striata, but it is divided into three stages of cultivation using three media, and the germination and rooting efficiency are still to be determined. to further improve

Method used

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  • A kind of method of rapidly propagating bletilla striata
  • A kind of method of rapidly propagating bletilla striata
  • A kind of method of rapidly propagating bletilla striata

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The method of rapid propagation of bletilla striata:

[0024] (1) Aseptic germination of seeds: select uncracked and mature Bletilla striata capsules, wash the surface of the capsules with detergent water, rinse with tap water, soak in 75% alcohol for 30S on the ultra-clean workbench, and rinse with sterile water for 3-4 Sterilize with 0.1% mercuric chloride for 8 minutes, then rinse with sterile water for 7-8 times, dry the surface water of the capsule with sterile paper, peel off the middle of the capsule, take out the seeds, and quickly inoculate them on the germination medium. Inoculate 10 bottles for each treatment, inoculate 4 clumps in each bottle, observe and record regularly, and analyze the statistical results after 20 days; culture conditions: culture temperature (25±1) ℃, light intensity 2000Lx, light time 10h·d-1; germination culture The base is: MS+6-BA 1mg / L+NAA0.8mg / L+sucrose 30g / L+agar 4g / L, pH5.8.

[0025] (2) Rooting culture of strong seedlings: inoc...

Embodiment 2

[0029] The method of rapid propagation of bletilla striata:

[0030] (1) Aseptic germination of seeds: select uncracked and mature Bletilla striata capsules, wash the surface of the capsules with detergent water, rinse with tap water, soak in 75% alcohol for 30S on the ultra-clean workbench, and rinse with sterile water for 3-4 Sterilize with 0.1% mercuric chloride for 8 minutes, then rinse with sterile water for 7-8 times, dry the surface water of the capsule with sterile paper, peel off the middle of the capsule, take out the seeds, and quickly inoculate them on the germination medium. Inoculate 10 bottles for each treatment, inoculate 4 clumps in each bottle, observe and record regularly, and analyze the statistical results after 20 days; culture conditions: culture temperature (25±1) ℃, light intensity 1800Lx, light time 10h·d-1; germination culture The base is: MS+6-BA1mg / L+NAA0.8mg / L+AC0.3g / L+proline 0g / L+sucrose 30g / L+agar 4g / L, pH5.8.

[0031] (2) Rooting culture of s...

Embodiment 3

[0039] The method of rapid propagation of bletilla striata:

[0040] (1) Aseptic germination of seeds: select uncracked and mature Bletilla striata capsules, wash the surface of the capsules with detergent water, rinse with tap water, soak in 75% alcohol for 30S on the ultra-clean workbench, and rinse with sterile water for 3-4 Sterilize with 0.1% mercuric chloride for 8-10 minutes, rinse with sterile water for 7-8 times, dry the surface moisture of the capsule with sterile paper, peel off the middle of the capsule, take out the seeds, and quickly inoculate them on the germination medium , inoculate 10 bottles for each treatment, inoculate 4 clumps in each bottle, observe and record regularly, and analyze the statistical results after 20 days; culture conditions: culture temperature 25±1℃, light intensity 1500Lx, light time 10h·d-1; germination culture The base is: MS+6-BA1.5mg / L+NAA0.8mg / L+AC0.5g / L+proline 0.3g / L+sucrose 30g / L+agar 4g / L, pH5.8.

[0041] (2) Rooting culture o...

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Abstract

The invention relates to a method for rapidly breeding bletilla striata. The method comprises the following steps: 1) selecting maturated and un-cracked capsule and performing aseptic processing, peeling the capsule and taking the seeds, rapidly inoculating the seeds to a germination medium; 2) strong seedling rooting culture: selecting plants with a length being about 1 cm, and inoculating the plants on the medium for culturing for 60-70 days; and 3) test-tube plantlet domestication and transplanting: performing hardening seedling on the rooted test-tube plantlet with the length being more than 10 cm for 3-4 days at normal temperature, washing agar, immersing the material by using carbendazim with a concentration being 50%, and transplanting the material to a matrix. The method has the advantages that the used steps are less, one-step seedling-formation is realized, and the method has great meaning for establishing an industrial bletilla striata breeding system, a production program is simplified, efficiency is increased, cost is reduced, a pollution rate generated during an inoculation process is reduced, and the production efficiency is relatively increased.

Description

technical field [0001] The invention belongs to the technical field of medicinal plant cultivation, and relates to a method for rapidly propagating bletilla striata. Background technique [0002] Bletilla striata (Bletilla striata) is a perennial herbaceous plant of the genus Bletilla striata in the family Orchidaceae. Bletilla striata not only has high ornamental value, but also is one of the commonly used Chinese medicinal materials in my country (Flora of China, 1999). Dried tubers are used as medicine, which has the effects of astringent, hemostasis, detumescence and granulation (Chinese Pharmacopoeia, 2015). In addition, bletilla striata stems also contain high content of bletilla striata gum, which has special viscosity characteristics and can be used as a thickener and suspending agent , humectants and co-emulsifiers are used in cosmetics and have good effects (Liu Guangbin et al., 2005; Ma Shihong et al., 2009), with a wide range of applications and a rising demand. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄刚周黎付燕万新屏郑晓峰何宪江李杰
Owner 黔东南民族职业技术学院
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