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Seedling-culturing method for performing freeze preservation of gloiopeltis algae through attaching organs

A technology of cryopreservation and fixer, which is applied in the fields of seaweed cultivation, botany equipment and methods, climate change adaptation, etc. It can solve the problems of high cost, long cultivation time, and delayed seedling cultivation time, so as to save costs and ensure normal growth Effect

Inactive Publication Date: 2018-07-13
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Seedlings are raised during the breeding season of seaweed, and the spores germinate into discs (fixation devices) and then placed in the sea for cultivation. In summer, they will die because they cannot germinate upright and the fixation devices are easily covered by other attachments; Land cooling cultivation, the cultivation time will be very long (especially in southern China), resulting in high cost
[0007] In order to solve the problem of crossing summer of seaweed seedlings of the genus Pisces, as Chinese patent 200810030121.5 discloses a "preservation and seedling method of seedlings of seaweeds of the genus Pisces", this method solves the problem of crossing over by air-drying and freezing the seaweeds. Summer problem; but there are defects in this method: due to air-drying and freezing the seed algae, the seedling time will be delayed, and the cultivated seedlings have not had time to grow into adults under the condition of bad weather (the rainy day lasts for a long time) It will usher in the annual high temperature season again, and the seedlings will not be able to grow further

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] During the algae breeding season of the genus Antler, select the antler seaweed to grow the algae, remove the miscellaneous algae and wash it with sea water; put it in the dark and precipitated sea water to release the spores.

[0022] Prepare a cultivation pool with seawater and a flat attachment base. The attachment base is made of hard materials such as plastic plates or plastic nets, and the spores released by the antler seaweed are evenly poured into the pool for seedling collection;

[0023] After the seedlings are picked, germinate and cultivate the spores on the attachment base, and take out the attachment base from the cultivation pool when the fixator grows to ≥ 250 μm;

[0024] Place the attachment base in an air-conditioned room to air dry for about two hours, set the temperature ≤ 18°C, until there is no visible moisture on the surface of the attachment base;

[0025] Place the attachment base in a plastic bag, seal it and store it in a freezer below -18°C....

Embodiment 2

[0029] During the breeding season of seaweed, choose seaweed to grow algae, remove miscellaneous algae and wash with sea water;

[0030] Prepare a cultivation pond with seawater and an attachment base made of cement boards, and evenly pour the spores released by the antler seaweed species into the pool for seedling collection;

[0031] After the seedlings are picked, germinate and cultivate the spores on the attachment base, and take out the attachment base from the cultivation pool when the fixator grows to ≥ 250 μm;

[0032] Place the attachment base in an air-conditioned room to air dry for about two hours, set the temperature ≤ 18°C, until there is no visible moisture on the surface of the attachment base;

[0033] Place the attachment base in a plastic bag, seal it and freeze it to below -18°C for storage.

[0034] In autumn and winter, when the ocean water temperature drops below 23°C, thaw the attachment base, put it into a pool filled with fresh seawater or place it i...

Embodiment 3

[0036] After harvesting the algae in the breeding stage of Example 1; place the attachment base with the retainer in an air-conditioned room for about two hours to air-dry, set the temperature ≤ 18°C ​​until there is no visible moisture on the surface of the attachment base; place it in a plastic bag Seal and freeze to below -18°C for further storage.

[0037] In the autumn and winter of the next year, when the ocean water temperature drops below 23°C, thaw the attachment base, put it into a pool filled with fresh seawater or place it in the sea; then enter the breeding stage in a conventional manner.

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PUM

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Abstract

The invention discloses a seedling-culturing method for performing freeze preservation through gloiopeltis algae through attaching organs. The method includes the following steps that, in pretreatment, gloiopeltis algae seeds are selected, impurities are removed, cleaning is performed, and spores are released; 2, in germination, attaching media are used for performing seedling collection on the spores, and the spores germinate and are cultured on the attaching media until the attaching organs are larger than or equal to 250 micrometers; in air drying, the attaching media and the gloiopeltis alga attaching organs are subjected to air drying until there is no water on surfaces, and then subjected to freeze preservation at the temperature of 18 DEG C or below; 4, in culturing, when the temperature of seawater is decreased to the temperature of 23 DEG C or below, and the attaching media and the attaching organs are taken out for culturing until straight bodies larger than or equal to 1 mmgrow out, and then aqaculture is performed. On the premise that in aquaculture of the gloiopeltis algae, survival from summer is realized, normal growth of the seedlings is guaranteed, the attaching organs can be used repeatedly, and the cost for seedling culturing is lowered.

Description

technical field [0001] The invention relates to a method for raising seedlings of algae, in particular to a method for raising seedlings of algae of the genus Pironella by freezing with a fixator. Background technique [0002] Seaweed (mainly seaweed and antler seaweed) are red algae with high economic value, edible, medicinal and industrial raw materials. But at present, the amount of seaweed algae resources is decreasing day by day, and the market demand is increasing day by day, so it is very important to develop the cultivation technology of seaweed. [0003] The shape of the algae of the genus Caroline is divided into an upright algae part and a base fixator part; the fixator is disc-shaped, also known as a disc-shaped body, which plays a role in fixing the algae body on the attachment base. [0004] To develop the cultivation of the seaweed, at first the source of the seedlings must be solved, and there is a key problem that must be solved in the cultivation of the se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G33/00
CPCA01G33/00Y02A40/80
Inventor 陈素文张文文朱长波李俊伟李婷张博苏家齐颉晓勇
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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