Seedling-culturing method for performing freeze preservation of gloiopeltis algae through attaching organs
A technology of cryopreservation and fixer, which is applied in the fields of seaweed cultivation, botany equipment and methods, climate change adaptation, etc. It can solve the problems of high cost, long cultivation time, and delayed seedling cultivation time, so as to save costs and ensure normal growth Effect
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Embodiment 1
[0021] During the algae breeding season of the genus Antler, select the antler seaweed to grow the algae, remove the miscellaneous algae and wash it with sea water; put it in the dark and precipitated sea water to release the spores.
[0022] Prepare a cultivation pool with seawater and a flat attachment base. The attachment base is made of hard materials such as plastic plates or plastic nets, and the spores released by the antler seaweed are evenly poured into the pool for seedling collection;
[0023] After the seedlings are picked, germinate and cultivate the spores on the attachment base, and take out the attachment base from the cultivation pool when the fixator grows to ≥ 250 μm;
[0024] Place the attachment base in an air-conditioned room to air dry for about two hours, set the temperature ≤ 18°C, until there is no visible moisture on the surface of the attachment base;
[0025] Place the attachment base in a plastic bag, seal it and store it in a freezer below -18°C....
Embodiment 2
[0029] During the breeding season of seaweed, choose seaweed to grow algae, remove miscellaneous algae and wash with sea water;
[0030] Prepare a cultivation pond with seawater and an attachment base made of cement boards, and evenly pour the spores released by the antler seaweed species into the pool for seedling collection;
[0031] After the seedlings are picked, germinate and cultivate the spores on the attachment base, and take out the attachment base from the cultivation pool when the fixator grows to ≥ 250 μm;
[0032] Place the attachment base in an air-conditioned room to air dry for about two hours, set the temperature ≤ 18°C, until there is no visible moisture on the surface of the attachment base;
[0033] Place the attachment base in a plastic bag, seal it and freeze it to below -18°C for storage.
[0034] In autumn and winter, when the ocean water temperature drops below 23°C, thaw the attachment base, put it into a pool filled with fresh seawater or place it i...
Embodiment 3
[0036] After harvesting the algae in the breeding stage of Example 1; place the attachment base with the retainer in an air-conditioned room for about two hours to air-dry, set the temperature ≤ 18°C until there is no visible moisture on the surface of the attachment base; place it in a plastic bag Seal and freeze to below -18°C for further storage.
[0037] In the autumn and winter of the next year, when the ocean water temperature drops below 23°C, thaw the attachment base, put it into a pool filled with fresh seawater or place it in the sea; then enter the breeding stage in a conventional manner.
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